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- Hydroxychloroquine an Antimalarial Drug, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Multitargeting
- Sargun Tushar Basrani, Tanjila Chandsaheb Gavandi, Shivani Balasaheb Patil, Nandkumar Subhash Kadam, Dhairyasheel Vasantrao Yadav, Sayali Ashok Chougule, Sankunny Mohan Karuppayil, Ashwini Khanderao Jadhav
- J. Microbiol. 2024;62(5):381-391. Published online April 8, 2024
- DOI: https://doi.org/10.1007/s12275-024-00111-6
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Abstract
- Candida albicans is the primary etiological agent associated with candidiasis in humans. Unrestricted growth of C. albicans can progress to systemic infections in the worst situation. This study investigates the antifungal activity of Hydroxychloroquine (HCQ) and mode of action against C. albicans. HCQ inhibited the planktonic growth and yeast to hyphal form morphogenesis of C. albicans significantly at 0.5 mg/ml concentration. The minimum inhibitory concentrations (MIC(50)) of HCQ for C. albicans adhesion and biofilm formation on the polystyrene surface was at 2 mg/ml and 4 mg/ml respectively. Various methods, such as scanning electron microscopy, exploration of the ergosterol biosynthesis pathway, cell cycle analysis, and assessment of S oxygen species (ROS) generation, were employed to investigate HCQ exerting its antifungal effects. HCQ was observed to reduce ergosterol levels in the cell membranes of C. albicans in a dose-dependent manner. Furthermore, HCQ treatment caused a substantial arrest of the C. albicans cell cycle at the G0/G1 phase, which impeded normal cell growth. Gene expression analysis revealed upregulation of SOD2, SOD1, and CAT1 genes after HCQ treatment, while genes like HWP1, RAS1, TEC1, and CDC 35 were downregulated. The study also assessed the in vivo efficacy of HCQ in a mice model, revealing a reduction in the pathogenicity of C. albicans after HCQ treatment. These results indicate that HCQ holds for the development of novel antifungal therapies.
- Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces
- Yuyuan Huang , Lingzhi Dong , Jian Gong , Jing Yang , Shan Lu , Xin-He Lai , Dong Jin , Qianni Huang , Ji Pu , Liyun Liu , Jianguo Xu
- J. Microbiol. 2022;60(10):977-985. Published online August 19, 2022
- DOI: https://doi.org/10.1007/s12275-022-2134-8
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- 3 Web of Science
- 4 Crossref
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Abstract
- Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°2054N, 106°4920E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°0910N, 102°0439E, October 2013) of South China, respectively. Optimal growth is obtained at 25–28°C (range, 4–32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0–10.0) in the presence of 0.5– 1.0% NaCl (w/v) (range, 0–15% NaCl [w/v]). The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7–46.8%; ANI, 91.9–92.1%). Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively. The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T. Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001. Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene. Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp. nov. is proposed (type strain HY170T = CGMCC 1.18600T = JCM 34230T).
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Citations
Citations to this article as recorded by
- Antagonistic Behavior of Streptomyces chartreuse against Pathogenic Bacteria in Ricinus communis L.
Bhoomi N. Patel, Priti Patel, Gayatri Patel
Biosciences Biotechnology Research Asia.2024; 21(1): 185. CrossRef - Functional genomics and taxonomic insights into heavy metal tolerant novel bacterium Brevibacterium metallidurans sp. nov. NCCP-602T isolated from tannery effluent in Pakistan
Sadia Manzoor, Saira Abbas, Sobia Zulfiqar, Hong-Chuan Wang, Min Xiao, Wen-Jun Li, Muhammad Arshad, Iftikhar Ahmed
Antonie van Leeuwenhoek.2024;[Epub] CrossRef - Description of Ornithinimicrobium cryptoxanthini sp. nov., a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments
Yuyuan Huang, Yifan Jiao, Sihui Zhang, Yuanmeihui Tao, Suping Zhang, Dong Jin, Ji Pu, Liyun Liu, Jing Yang, Shan Lu
Journal of Microbiology.2023; 61(4): 379. CrossRef - Morphological and genomic characteristics of two novel actinomycetes, Ornithinimicrobium sufpigmenti sp. nov. and Ornithinimicrobium faecis sp. nov. isolated from bat faeces (Rousettus leschenaultia and Taphozous perforates)
Yuyuan Huang, Suping Zhang, Yuanmeihui Tao, Jing Yang, Shan Lu, Dong Jin, Ji Pu, Wenbo Luo, Han Zheng, Liyun Liu, Jia-fu Jiang, Jianguo Xu
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
- Antagonistic Behavior of Streptomyces chartreuse against Pathogenic Bacteria in Ricinus communis L.
- Comparative analysis of type 2 diabetes-associated gut microbiota between Han and Mongolian people
- Shu-chun Li , Yao Xiao , Ri-tu Wu , Dan Xie , Huan-hu Zhao , Gang-yi Shen , En-qi Wu
- J. Microbiol. 2021;59(7):693-701. Published online May 15, 2021
- DOI: https://doi.org/10.1007/s12275-021-0454-8
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- 19 Web of Science
- 14 Crossref
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Abstract
- Due to the different rates of diabetes in different ethnic groups and the structural differences in intestinal microbiota, this study evaluated the changes in diabetes-related intestinal microbiota in two ethnic groups. Fifty-six stool samples were collected from subjects from the Han and Mongolian ethnic groups in China, including participants without diabetes (non-diabetic, ND) and with type 2 diabetes (T2D). The 16S rDNA gene V3 + V4 area was extracted from microbiota, amplified by PCR, and used to perform high-throughput sequencing and screen differential microbiota associated with ethnicity. The results showed that there were 44 T2D-related bacterial markers in the Han subjects, of which Flavonifractor, Alistipes, Prevotella, Oscillibacter, Clostridium XlVa, and Lachnospiracea_incertae_sedis were most closely related to diabetes. There were 20 T2D-related bacterial markers in the Mongolian subjects, of which Fastidiosipila and Barnesiella were most closely related to diabetes. The common markers of T2D bacteria in the two ethnic groups were Papillibacter and Bifidobacterium. There were 17 metabolic pathways with significant differences between the ND and T2D groups in the Han group, and 29 metabolic pathways in the Mongolian group. The glutamatergic metabolic pathway was the only common metabolic pathway in two ethnic groups. The composition and function of diabetes-related bacteria were significantly different among the different ethnic groups, which suggested that the influence of ethnic differences should be fully considered when studying the association between diabetes and bacteria. In addition, the common bacterial markers found in diabetic patients of different ethnic groups in this study can be used as potential targets to study the pathogenesis and treatment of diabetes.
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Citations
Citations to this article as recorded by- Pentachlorophenol increases diabetes risk by damaging β-cell secretion and disrupting gut microbial-related amino acids and fatty acids biosynthesis
Muke Han, Jie Yin, Xinyi Wang, Runhui Yang, Zhong Dong, Junyu Ning, Yajun Xu, Bing Shao
Journal of Hazardous Materials.2024; 480: 136103. CrossRef - Improvement of glucose metabolism disorder by wheat dietary fibre depended on the intake mode and regulated through TLRs/NF-κB/TNF pathway in db/db mice
Xinguo Liu, Shaojie Pang, Aoxiang Li, Yong Wang, Wei Fang, Wentao Qi
Food and Agricultural Immunology.2024;[Epub] CrossRef - Chemical modifications of xylan from sugarcane bagasse and their regulatory effects on gut microbiota in mice
Guozhu Zhang, Yuan Guan, Xin Zhang, Jing Li, Haishan Chen, Li Zhou, Jun Liang, Xia Li
Journal of Carbohydrate Chemistry.2024; 43(3): 90. CrossRef - Tangshen Formula alleviates inflammatory injury against aged diabetic kidney disease through modulating gut microbiota composition and related amino acid metabolism
Dan-Qian Chen, Hao-Jun Zhang, Wen Zhang, Kai Feng, Hui Liu, Hai-Ling Zhao, Ping Li
Experimental Gerontology.2024; 188: 112393. CrossRef - Differences in gut microbiota and its metabolic function among different fasting plasma glucose groups in Mongolian population of China
Yanchao Liu, Mingxiao Wang, Wuyuntana Li, Yumin Gao, Hailing Li, Ning Cao, Wenli Hao, Lingyan Zhao
BMC Microbiology.2023;[Epub] CrossRef - Bi-Directional Interactions between Glucose-Lowering Medications and Gut Microbiome in Patients with Type 2 Diabetes Mellitus: A Systematic Review
Ruolin Li, Fereshteh Shokri, Alejandro Rincon, Fernando Rivadeneira, Carolina Medina-Gomez, Fariba Ahmadizar
Genes.2023; 14(8): 1572. CrossRef - Microbe-Disease Association Prediction Using RGCN Through Microbe-Drug-Disease Network
Yueyue Wang, Xiujuan Lei, Yi Pan
IEEE/ACM Transactions on Computational Biology and Bioinformatics.2023; 20(6): 3353. CrossRef - Longitudinal Characterization of the Gut Microbiota in the Diabetic ZDSD Rat Model and Therapeutic Potential of Oligofructose
Savanna N. Weninger, Angela Ding, Elizabeth N. Browne, Morgan L. Frost, Gabriele Schiro, Daniel Laubitz, Frank A. Duca
Metabolites.2023; 13(5): 660. CrossRef - Effect of Benaglutide on Gut Microbiota and Fecal Metabolites in Patients with Type 2 Diabetes Mellitus
Chen-Yu Han, Xiao-Mei Ye, Jia-Ping Lu, Hai-Ying Jin, Ping Wang, Wei-Wei Xu, Min Zhang
Diabetes, Metabolic Syndrome and Obesity.2023; Volume 16: 2329. CrossRef - Alteration of intestinal microbiota is associated with diabetic retinopathy and its severity: Samples collected from southeast coast Chinese
Xue-Mei Gu, Chao-Yin Lu, Jian Pan, Jian-Zhong Ye, Qi-Han Zhu
World Journal of Diabetes.2023; 14(6): 862. CrossRef - Progress in research on gut microbiota in ethnic minorities in China and consideration of intervention strategies based on ethnic medicine: A review
Rong Chen, Zhong-Yu Duan, Xiao-Hua Duan, Qing-Hua Chen, Jin Zheng
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - Effects of Oral Glucose-Lowering Agents on Gut Microbiota and Microbial Metabolites
Dongmei Wang, Jieying Liu, Liyuan Zhou, Qian Zhang, Ming Li, Xinhua Xiao
Frontiers in Endocrinology.2022;[Epub] CrossRef - Depletion of gut secretory immunoglobulin A coatedLactobacillus reuteriis associated with gestational diabetes mellitus-related intestinal mucosal barrier damage
Haowen Zhang, Ce Qi, Yuning Zhao, Mengyao Lu, Xinyue Li, Jingbo Zhou, Hongyang Dang, Mengjun Cui, Tingting Miao, Jin Sun, Duo Li
Food & Function.2021; 12(21): 10783. CrossRef - The Microbiota Profile Analysis of Combined Periodontal-Endodontic Lesions Using 16S rRNA Next-Generation Sequencing
Ping Sun, Zhiyong Guo, Daiping Guo, Jian Wang, Tingting Wu, Tingjun Li, Jiannan Liu, Xinhua Liu, Jôice D. Corrêa
Journal of Immunology Research.2021; 2021: 1. CrossRef
- Pentachlorophenol increases diabetes risk by damaging β-cell secretion and disrupting gut microbial-related amino acids and fatty acids biosynthesis
- N-acetylcysteine prevents the development of gastritis induced by Helicobacter pylori infection
- Sungil Jang , Eun-Jung Bak , Jeong-Heon Cha
- J. Microbiol. 2017;55(5):396-402. Published online April 29, 2017
- DOI: https://doi.org/10.1007/s12275-017-7089-9
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- 13 Crossref
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Abstract
- Helicobacter pylori (H. pylori) is a human gastric pathogen, causing various gastric diseases ranging from gastritis to gas-tric adenocarcinoma. It has been reported that combining N-acetylcysteine (NAC) with conventional antibiotic therapy increases the success rate of H. pylori eradication. We evalu-ated the effect of NAC itself on the growth and coloniza-tion of H. pylori, and development of gastritis, using in vitro liquid culture system and in vivo animal models. H. pylori growth was evaluated in broth culture containing NAC. The H. pylori load and histopathological scores of stomachs were measured in Mongolian gerbils infected with H. pylori strain 7.13, and fed with NAC-containing diet. In liquid culture, NAC inhibited H. pylori growth in a concentration-depen-dent manner. In the animal model, 3-day administration of NAC after 1 week from infection reduced the H. pylori load; 6-week administration of NAC after 1 week from infection prevented the development of gastritis and reduced H. pylori colonization. However, no reduction in the bacterial load or degree of gastritis was observed with a 6-week administ-ration of NAC following 6-week infection period. Our results indicate that NAC may exert a beneficial effect on reduction of bacterial colonization, and prevents the development of severe inflammation, in people with initial asymptomatic or mild H. pylori infection.
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Citations
Citations to this article as recorded by- Role of N-acetylcysteine and vitamin B complex in improving outcomes of corrosive ingestion
Heidi El-Sobky, Safaa Mostafa El-Shanawany, Maha Ghanem, Maram Atef
Toxicology Research.2024;[Epub] CrossRef - Helicobacter pylori: Routes of Infection, Antimicrobial Resistance, and Alternative Therapies as a Means to Develop Infection Control
Ayman Elbehiry, Adil Abalkhail, Nuha Anajirih, Fahad Alkhamisi, Mohammed Aldamegh, Abdullah Alramzi, Riyad AlShaqi, Naif Alotaibi, Abdullah Aljuaid, Hilal Alzahrani, Feras Alzaben, Mohammed Rawway, Mai Ibrahem, Moustafa H. Abdelsalam, Nermin I. Rizk, Moha
Diseases.2024; 12(12): 311. CrossRef - Isotope tracing reveals bacterial catabolism of host-derived glutathione during Helicobacter pylori infection
Maia J. Baskerville, Yekaterina Kovalyova, Raquel Mejías-Luque, Markus Gerhard, Stavroula K. Hatzios, William Navarre
PLOS Pathogens.2023; 19(7): e1011526. CrossRef - Helicobacter pylori-mediated gastric pathogenesis is attenuated by treatment of 2-deoxyglucose and metformin
Hanfu Su, Eun-Jung Bak, Aeryun Kim, Kavinda Tissera, Jeong-Heon Cha, Sungil Jang
Journal of Microbiology.2022; 60(8): 849. CrossRef - Population Pharmacokinetics of Doxycycline, Administered Alone or with N-Acetylcysteine, in Chickens with Experimental Mycoplasma gallisepticum Infection
Tsvetelina Petkova, Antoaneta Yordanova, Aneliya Milanova
Pharmaceutics.2022; 14(11): 2440. CrossRef - N-Acetylcysteine: A Review of Clinical Usefulness (an Old Drug with New Tricks)
Gerry K. Schwalfenberg, Tatiana Emanuelli
Journal of Nutrition and Metabolism.2021; 2021: 1. CrossRef - Enhanced Eradication of Mucin‐Embedded Bacterial Biofilm by Locally Delivered Antibiotics in Functionalized Microcontainers
Stine Egebro Birk, Laura Serioli, Valentina Cavallo, Janus Anders Juul Haagensen, Søren Molin, Line Hagner Nielsen, Kinga Zór, Anja Boisen
Macromolecular Bioscience.2021;[Epub] CrossRef - N-Acetylcysteine (NAC): Impacts on Human Health
Micaely Cristina dos Santos Tenório, Nayara Gomes Graciliano, Fabiana Andréa Moura, Alane Cabral Menezes de Oliveira, Marília Oliveira Fonseca Goulart
Antioxidants.2021; 10(6): 967. CrossRef - N-acetylcysteine blocks SOS induction and mutagenesis produced by fluoroquinolones in Escherichia coli
Ana I Rodríguez-Rosado, Estela Ynés Valencia, Alexandro Rodríguez-Rojas, Coloma Costas, Rodrigo S Galhardo, Jerónimo Rodríguez-Beltrán, Jesús Blázquez
Journal of Antimicrobial Chemotherapy.2019; 74(8): 2188. CrossRef - N-Acetylcysteine Ameliorates Gentamicin-Induced Nephrotoxicity by Enhancing Autophagy and Reducing Oxidative Damage in Miniature Pigs
Jing Cui, Li Tang, Quan Hong, Shupeng Lin, Xuefeng Sun, Guangyan Cai, Xue-Yuan Bai, Xiangmei Chen
Shock.2019; 52(6): 622. CrossRef - Allantoin improves histopathological evaluations in a rat model of gastritis
M Eslami-farsani, A Moslehi, A Hatami-shahmir
Physiology International.2018; 105(4): 325. CrossRef - N‐Acetylcysteine Reduces ROS‐Mediated Oxidative DNA Damage and PI3K/Akt Pathway Activation Induced by Helicobacter pylori Infection
Chuan Xie, Jian Yi, Jing Lu, Muwen Nie, Meifang Huang, Jianfang Rong, Zhenhua Zhu, Jiang Chen, Xiaoliang Zhou, Bimin Li, Haiming Chen, Nonghua Lu, Xu Shu, Rômulo D. Novaes
Oxidative Medicine and Cellular Longevity.2018;[Epub] CrossRef - Oral Immunization with a Multivalent Epitope-Based Vaccine, Based on NAP, Urease, HSP60, and HpaA, Provides Therapeutic Effect on H. pylori Infection in Mongolian gerbils
Le Guo, Hua Yang, Feng Tang, Runting Yin, Hongpeng Liu, Xiaojuan Gong, Jun Wei, Ying Zhang, Guangxian Xu, Kunmei Liu
Frontiers in Cellular and Infection Microbiology.2017;[Epub] CrossRef
- Role of N-acetylcysteine and vitamin B complex in improving outcomes of corrosive ingestion
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