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- RNA Interference Targeting Nucleocapsid Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication in Marc-145 Cells
- Minnan Yang , Qun Xiang , Xiaodong Zhang , Xiang Li , Seydou Sylla , Zhuang Ding
- J. Microbiol. 2014;52(4):333-339. Published online March 29, 2014
- DOI: https://doi.org/10.1007/s12275-014-3419-3
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- 6 Crossref
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Abstract
- Porcine reproductive and respiratory syndrome (PRRS) is an important disease, which leads to severe economic losses in swine-producing areas of the world. However, current antiviral strategies cannot provide highly effective protection. In this study, three theoretically effective interference target sites (71-91, 144-164, 218-238) targeting the nucleocapsid (N) gene of PRRSV were designed and selected, and then three siRNA-expressing plasmids were constructed, respectively named p2.1-N71, p2.1-N144, and p2.1-N218. The recombinant siRNA-expressing plasmids were transfected into Marc-145 cells; then the cells were infected with PRRSV (JL07SW strain); finally, after incubation for 48 h, the antiviral activity of those siRNA-expressing plasmids in Marc-145 cells was assessed by cytopathic effects, virus titers, indirect immunofluorescence, and quantitative real-time PCR. Experimental results demonstrated that these three siRNA-expressing plasmids could effectively and significantly inhibit the replication of PRRSV by 93.2%, 83.6%, and 89.2% in Marc-145 cells, respectively. Among these three siRNA-expressing plasmids, p2.1-N71 was found to be most effective, while p2.1-N144 and p2.1-N218 displayed relatively weak inhibition of virus replication. The results indicated that siRNA-expressing plasmids targeting the N gene of PRRSV could significantly inhibit PRRSV replication in Marc-145 cells. Based on our experimental results and previous reports, the 71-91, 179-197, and 234-252 sites of the N gene are good choices to effectively inhibit the replication of PRRSV, and this RNA interference technique can be a potential anti-PRRSV strategy.
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Citations
Citations to this article as recorded by
- Role of microRNAs in host defense against porcine reproductive and respiratory syndrome virus infection: a hidden front line
Xuewei Huang, Weiye Liu
Frontiers in Immunology.2024;[Epub] CrossRef - Porcine reproductive and respiratory syndrome virus infection induces microRNA novel-216 production to facilitate viral-replication by targeting MAVS 3´UTR
Xuegang Luo, Sha Xie, Xingsheng Xu, Yao Zhang, Yun Huang, Dongmei Tan, Yi Tan
Veterinary Microbiology.2024; 292: 110061. CrossRef - Antiviral Strategies against PRRSV Infection
Taofeng Du, Yuchen Nan, Shuqi Xiao, Qin Zhao, En-Min Zhou
Trends in Microbiology.2017; 25(12): 968. CrossRef - Anti-PRRSV effect and mechanism of tetrahydroaltersolanol Cin vitro
Song-Lin Zhang, Yi-Chun Wu, Fan Cheng, Zhi-Yong Guo, Jian-Feng Chen
Journal of Asian Natural Products Research.2016; 18(3): 303. CrossRef - Cellular microRNA miR-26a suppresses replication of porcine reproductive and respiratory syndrome virus by activating innate antiviral immunity
Xiaojuan Jia, Yuhai Bi, Jing Li, Qing Xie, Hanchun Yang, Wenjun Liu
Scientific Reports.2015;[Epub] CrossRef - Inhibition of porcine reproductive and respiratory syndrome virus replication in vitro using DNA-based short antisense oligonucleotides
Longlong Zheng, Xiang Li, Lingyun Zhu, Wengui Li, Junlong Bi, Guishu Yang, Gefen Yin, Jianping Liu
BMC Veterinary Research.2015;[Epub] CrossRef
- Role of microRNAs in host defense against porcine reproductive and respiratory syndrome virus infection: a hidden front line
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