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- Iron interferes with quorum sensing-mediated cooperation in Pseudomonas aeruginosa by affecting the expression of ppyR and mexT, in addition to rhlR
- Feng Sun , Na Li , Lijia Wang , Huajun Feng , Dongsheng Shen , Meizhen Wang
- J. Microbiol. 2020;58(11):938-944. Published online October 30, 2020
- DOI: https://doi.org/10.1007/s12275-020-0264-4
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- 4 Web of Science
- 4 Crossref
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Abstract
- The stabilization of quorum sensing (QS) is vital for bacterial survival in various environments. Although the mechanisms of QS stabilization in certain conditions have been well studied, the impact of environmental factors has received much less attention. In this study, we show that the supplementation of 25 μM iron in competition experiments and 50 μM in evolution experiments to casein growth cultures significantly increased the possibility of population collapse by affecting elastase production. However, the expression of lasI and lasR remained constant regardless of iron concentration and hence this effect was not through interference with the LasIR circuit, which mainly regulates the secretion of elastase in Pseudomonas aeruginosa. However, the expression of rhlR was significantly inhibited by iron treatment, which could affect the production of elastase. Further, based on both reverse transcription quantitative polymerase chain reaction and gene knock-out assays, we show that iron inhibits the transcription of ppyR and enhances the expression of mexT, both of which decrease elastase production and correspondingly interfere with QS stabilization. Our findings show that environmental factors can affect the genes of QS circuits, interfering with QS stabilization. These findings are not only beneficial in understanding the mechanistic effect of iron on QS stabilization, but also demonstrate the complexity of QS stabilization by linking non-QS-related genes with QS traits.
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Citations
Citations to this article as recorded by
- Effect of LuxS/AI-2-mediated quorum sensing system on bacteriocin production of Lactobacillus plantarum NMD-17
Li-Li Man, Dian-Jun Xiang
Folia Microbiologica.2023; 68(6): 855. CrossRef - PtsN in Pseudomonas aeruginosa Is Phosphorylated by Redundant Upstream Proteins and Impacts Virulence-Related Genes
Simon A. M. Underhill, Somalisa Pan, Mary Erdmann, Matthew T. Cabeen, Joseph Bondy-Denomy
Journal of Bacteriology.2023;[Epub] CrossRef - The immune responses to different Uropathogens call individual interventions for bladder infection
Linlong Li, Yangyang Li, Jiali Yang, Xiang Xie, Huan Chen
Frontiers in Immunology.2022;[Epub] CrossRef - Design of Polymeric Thin Films to Direct Microbial Biofilm Growth, Virulence, and Metabolism
Trevor Franklin, Yinan Wu, Jiayan Lang, Sijin Li, Rong Yang
Biomacromolecules.2021; 22(12): 4933. CrossRef
- Effect of LuxS/AI-2-mediated quorum sensing system on bacteriocin production of Lactobacillus plantarum NMD-17
- Middle East respiratory syndrome coronavirus-encoded ORF8b strongly antagonizes IFN-β promoter activation: its implication for vaccine design
- Jeong Yoon Lee , Sojung Bae , Jinjong Myoung
- J. Microbiol. 2019;57(9):803-811. Published online August 27, 2019
- DOI: https://doi.org/10.1007/s12275-019-9272-7
- 55 View
- 0 Download
- 37 Web of Science
- 32 Crossref
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Abstract
- Middle East respiratory syndrome coronavirus (MERS-CoV) is a causative agent of severe-to-fatal pneumonia especially in patients with pre-existing conditions, such as smoking and chronic obstructive pulmonary disease (COPD). MERS-CoV transmission continues to be reported in the Saudi Arabian Peninsula since its discovery in 2012. However, it has rarely been epidemic outside the area except one large outbreak in South Korea in May 2015. The genome of the epidemic MERS-CoV isolated from a Korean patient revealed its homology to previously reported strains. MERS-CoV encodes 5 accessory proteins and generally, they do not participate in the genome transcription and replication but rather are involved in viral evasion of the host innate immune responses. Here we report that ORF8b, an accessory protein of MERSCoV, strongly inhibits both MDA5- and RIG-I-mediated activation of interferon beta promoter activity while downstream signaling molecules were left largely unaffected. Of note, MDA5 protein levels were significantly down-regulated by ORF8b and co-expression of ORF4a and ORF4b. These novel findings will facilitate elucidation of mechanisms of virus-encoded evasion strategies, thus helping design rationale antiviral countermeasures against deadly MERS-CoV infection.
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Citations
Citations to this article as recorded by- MDA5 Is a Major Determinant of Developing Symptoms in Critically Ill COVID-19 Patients
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Journal of Microbiology.2022; 60(3): 335. CrossRef - The Interplay Between Coronavirus and Type I IFN Response
Wenxiang Xue, Chan Ding, Kun Qian, Ying Liao
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Luis Enjuanes, Isabel Sola, Sonia Zúñiga, José M. Honrubia, Melissa Bello-Pérez, Alejandro Sanz-Bravo, Ezequiel González-Miranda, Jesús Hurtado-Tamayo, Ricardo Requena-Platek, Li Wang, Diego Muñoz-Santos, Carlos M. Sánchez, Ana Esteban, Jorge Ripoll-Gómez
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Yuming Li, Yingkang Jin, Lijun Kuang, Zhenhua Luo, Fang Li, Jing Sun, Airu Zhu, Zhen Zhuang, Yanqun Wang, Liyan Wen, Donglan Liu, Chunke Chen, Mian Gan, Jingxian Zhao, Jincun Zhao, Tom Gallagher
Journal of Virology.2022;[Epub] CrossRef - Middle East respiratory syndrome coronavirus ORF4b protein inhibits TLR7‐ and TLR9‐dependent alpha interferon induction
Yoshinori Kitagawa, Takumi Tsukamoto, Masae Itoh, Bin Gotoh
FEBS Letters.2022; 596(19): 2538. CrossRef - Escape and Over-Activation of Innate Immune Responses by SARS-CoV-2: Two Faces of a Coin
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J. Gutiérrez-Álvarez, J. M. Honrubia, A. Sanz-Bravo, E. González-Miranda, R. Fernández-Delgado, M. T. Rejas, S. Zúñiga, I. Sola, L. Enjuanes
Proceedings of the National Academy of Sciences.2021;[Epub] CrossRef - The roles of signaling pathways in SARS-CoV-2 infection; lessons learned from SARS-CoV and MERS-CoV
Nima Hemmat, Zahra Asadzadeh, Noora Karim Ahangar, Hajar Alemohammad, Basira Najafzadeh, Afshin Derakhshani, Amir Baghbanzadeh, Hossein Bannazadeh Baghi, Darya Javadrashid, Souzan Najafi, Meriadeg Ar Gouilh, Behzad Baradaran
Archives of Virology.2021; 166(3): 675. CrossRef - Interspecies Jumping of Bat Coronaviruses
Antonio C. P. Wong, Susanna K. P. Lau, Patrick C. Y. Woo
Viruses.2021; 13(11): 2188. CrossRef - Chikungunya and Zika Viruses: Co-Circulation and the Interplay between Viral Proteins and Host Factors
Sineewanlaya Wichit, Nuttamonpat Gumpangseth, Rodolphe Hamel, Sakda Yainoy, Siwaret Arikit, Chuchard Punsawad, Dorothée Missé
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Javier Gutiérrez-Álvarez, José M. Honrubia, Raúl Fernández-Delgado, Li Wang, Carlos Castaño-Rodríguez, Sonia Zúñiga, Isabel Sola, Luis Enjuanes, Mark R. Dennison
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Rohini S. Kharwade, Sachin M. More
Coronaviruses.2021; 2(4): 468. CrossRef - A Promising Vaccination Strategy against COVID-19 on the Horizon: Heterologous Immunization
Sameer-ul-Salam Mattoo, Jinjong Myoung
Journal of Microbiology and Biotechnology.2021; 31(12): 1601. CrossRef - The ORF8 protein of SARS-CoV-2 induced endoplasmic reticulum stress and mediated immune evasion by antagonizing production of interferon beta
Farooq Rashid, Emmanuel Enoch Dzakah, Haiying Wang, Shixing Tang
Virus Research.2021; 296: 198350. CrossRef - Development of A MERS-CoV Replicon Cell Line for Antiviral Screening
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Pathogens.2020; 9(5): 376. CrossRef - Zika Virus-Encoded NS2A and NS4A Strongly Downregulate NF-κB Promoter Activity
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Sojung Bae, Jeong Yoon Lee, Jinjong Myoung
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- MDA5 Is a Major Determinant of Developing Symptoms in Critically Ill COVID-19 Patients
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