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- Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense
- Syahriel Abdullah , Yoon Sin Oh , Min-Kyu Kwak , KhimPhin Chong
- J. Microbiol. 2021;59(2):164-174. Published online December 23, 2020
- DOI: https://doi.org/10.1007/s12275-021-0551-8
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- 8 Web of Science
- 7 Crossref
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Abstract
-
There have been relatively few studies which support a link
between Ganoderma boninense, a phytopathogenic fungus
that is particularly cytotoxic and pathogenic to plant tissues
and roots, and antimicrobial compounds. We previously observed
that liquid-liquid extraction (LLE) using chloroformmethanol-
water at a ratio (1:1:1) was superior at detecting
antibacterial activities and significant quantities of antibacterial
compounds. Herein, we demonstrate that antibacterial
secondary metabolites are produced from G. boninense mycelia.
Antibacterial compounds were monitored in concurrent
biochemical and biophysical experiments. The combined
methods
included high performance thin-layer chromatography (HPTLC), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR) spectroscopy. The antibacterial compounds derived from mycelia with chloroform-methanol extraction through LLE were isolated via a gradient solvent elution system using HPTLC. The antibacterial activity of the isolated compounds was observed to be the most potent against Staphylococcus aureus ATCC 25923 and multidrug-resistant S. aureus NCTC 11939. GC-MS, HPLC, and FTIR analysis confirmed two antibacterial compounds, which were identified as 4,4,14α-trimethylcholestane (m/z = 414.75; lanostane, C30H54) and ergosta-5,7,22-trien-3β-ol (m/z = 396.65; ergosterol, C28H44O). With the aid of spectroscopic evaluations, ganoboninketal (m/z = 498.66, C30H42O6), which belongs to the 3,4-seco-27-norlanostane triterpene family, was additionally characterized by 2D-NMR analysis. Despite the lack of antibacterial potential exhibited by lanostane; both ergosterol and ganoboninketal displayed significant antibacterial activities against bacterial pathogens. Results provide evidence for the existence of bioactive compounds in the mycelia of the relatively unexplored phytopathogenic G. boninense, together with a robust method for estimating the corresponding potent antibacterial secondary metabolites. -
Citations
Citations to this article as recorded by
- Anti-Staphylococcus aureus potential of compounds from Ganoderma sp.: A comprehensive molecular docking and simulation approaches
Trang Thi Thu Nguyen, Trinh Thi Tuyet Nguyen, Hoang Duc Nguyen, Tan Khanh Nguyen, Phu Tran Vinh Pham, Linh Thuy Thi Tran, Hong Khuyen Thi Pham, Phu Chi Hieu Truong, Linh Thuoc Tran, Manh Hung Tran
Heliyon.2024; 10(7): e28118. CrossRef - Medium composition optimization and characterization of polysaccharides extracted from Ganoderma boninense along with antioxidant activity
Qian-Zhu Li, Chuan Xiong, Wei Chee Wong, Li-Wei Zhou
International Journal of Biological Macromolecules.2024; 260: 129528. CrossRef - Plant Defense Inducers and Antioxidant Metabolites Produced During Oil Palm-Ganoderma boninense Interaction In Vitro
Neda Shokrollahi, Chai-Ling Ho, Nur Ain Izzati Mohd Zainudin, Mohd As’wad Bin Abdul Wahab, Mui-Yun Wong
Chemistry Africa.2023; 6(1): 499. CrossRef - Identification of Antibacterial Metabolites from Endophytic Fungus Aspergillus fumigatus, Isolated from Albizia lucidior Leaves (Fabaceae), Utilizing Metabolomic and Molecular Docking Techniques
Mai E. Hussein, Osama G. Mohamed, Ahlam M. El-Fishawy, Hesham I. El-Askary, Amira S. El-Senousy, Ahmed A. El-Beih, Eman S. Nossier, Ahmed M. Naglah, Abdulrahman A. Almehizia, Ashootosh Tripathi, Ahmed A. Hamed
Molecules.2022; 27(3): 1117. CrossRef - Bioactive Compounds of Ganoderma boninense Inhibited Methicillin-Resistant Staphylococcus aureus Growth by Affecting Their Cell Membrane Permeability and Integrity
Yow-San Chan, Khim-Phin Chong
Molecules.2022; 27(3): 838. CrossRef - Review Update on the Life Cycle, Plant–Microbe Interaction, Genomics, Detection and Control Strategies of the Oil Palm Pathogen Ganoderma boninense
Izwan Bharudin, Anis Farhan Fatimi Ab Wahab, Muhammad Asyraff Abd Samad, Ng Xin Yie, Madihah Ahmad Zairun, Farah Diba Abu Bakar, Abdul Munir Abdul Murad
Biology.2022; 11(2): 251. CrossRef - Screening for Antibacterial Activity of French Mushrooms against Pathogenic and Multidrug Resistant Bacteria
Clément Huguet, Mélanie Bourjot, Jean-Michel Bellanger, Gilles Prévost, Aurélie Urbain
Applied Sciences.2022; 12(10): 5229. CrossRef
- Anti-Staphylococcus aureus potential of compounds from Ganoderma sp.: A comprehensive molecular docking and simulation approaches
- β-1,3-Glucan/CR3/SYK pathway-dependent LC3B-II accumulation enhanced the fungicidal activity in human neutrophils
- Ding Li , Changsen Bai , Qing Zhang , Zheng Li , Di Shao , Xichuan Li
- J. Microbiol. 2019;57(4):263-270. Published online February 5, 2019
- DOI: https://doi.org/10.1007/s12275-019-8298-1
- 54 View
- 0 Download
- 7 Web of Science
- 7 Crossref
-
Abstract
- Since molecular genotyping has been established for the Candida species, studies have found that a single Candida strain (endemic strain) can persist over a long period of time and results in the spread of nosocomial invasive candidiasis without general characteristics of horizontal transmissions. Our previous study also found the existence of endemic strains in a cancer center in Tianjin, China. In the current study, we performed further investigation on endemic and non-endemic Candida albicans strains, with the aim of explaining the higher morbidity of endemic strains. In an in vivo experiment, mice infected with endemic strains showed significantly shorter survival time and higher kidney fungal burdens compared to mice infected with non-endemic strains. In an in vitro experiment, the killing percentage of neutrophils to endemic strains was significantly lower than that to non-endemic strains, which is positively linked to the ratio of LC3B-II/I in neutrophils. An immunofluorescence assay showed more β-1,3-glucan exposure on the cell walls of nonendemic strains compared to endemic strains. After blocking the β-glucan receptor (CR3) or inhibiting downstream kinase (SYK) in neutrophils, the killing percent to C. albicans (regardless of endemic and non-endemic strains) and the ratio of LC3B-II/I of neutrophils were significantly decreased. These data suggested that the killing capability of neutrophils to C. albicans was monitored by β-1,3-glucan via CR3/SYK pathway-dependent LC3B-II accumulation and provided an explanation for the variable killing capability of neutrophils to different strains of C. albicans, which would be beneficial in improving infection control and therapeutic strategies for invasive candidiasis.
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Citations
Citations to this article as recorded by- LC3B: A microtubule-associated protein influences disease progression and prognosis
Yan Chen, Hong Yi, Shan Liao, Junyu He, Yanhong Zhou, Yan Lei
Cytokine & Growth Factor Reviews.2024;[Epub] CrossRef - Autophagy and LC3-associated phagocytosis contribute negatively to the killing capability of THP-1-derived macrophages against Candida albicans at the mid-stage
Ding Li, Lin Wang, Zhihong Zhao, Changsen Bai, Xichuan Li
Immunology Letters.2023; 263: 25. CrossRef - Metabolism and Biodegradation of β-Glucan in vivo
Ziming Zheng, Wenqi Tang, Weipeng Lu, Xu Mu, Yuxuan Liu, Xianglin Pan, Kaiping Wang, Yu Zhang
Frontiers in Veterinary Science.2022;[Epub] CrossRef - Biological Effects of β-Glucans on Osteoclastogenesis
Wataru Ariyoshi, Shiika Hara, Ayaka Koga, Yoshie Nagai-Yoshioka, Ryota Yamasaki
Molecules.2021; 26(7): 1982. CrossRef - Interaction Between Dendritic Cells and Candida krusei β-Glucan Partially Depends on Dectin-1 and It Promotes High IL-10 Production by T Cells
Truc Thi Huong Dinh, Phawida Tummamunkong, Panuwat Padungros, Pranpariya Ponpakdee, Lawan Boonprakong, Wilasinee Saisorn, Asada Leelahavanichkul, Patipark Kueanjinda, Patcharee Ritprajak
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef - Insights on the Functional Role of Beta-Glucans in Fungal Immunity Using Receptor-Deficient Mouse Models
Mark Joseph Maranan Desamero, Soo-Hyun Chung, Shigeru Kakuta
International Journal of Molecular Sciences.2021; 22(9): 4778. CrossRef - Immunomodulatory Effect of Structurally Characterized Mushroom Sclerotial Polysaccharides Isolated from Polyporus rhinocerus on Bone Marrow Dendritic Cells
Chaoran Liu, Man Wing Choi, Xingkui Xue, Peter C. K. Cheung
Journal of Agricultural and Food Chemistry.2019; 67(43): 12137. CrossRef
- LC3B: A microtubule-associated protein influences disease progression and prognosis
Review
- Bovine Viral Diarrhea Virus Infection Induces Autophagy in MDBK Cells
- Qiang Fu , Huijun Shi , Yan Ren , Fei Guo , Wei Ni , Jun Qiao , Pengyan Wang , Hui Zhang , Chuangfu Chen
- J. Microbiol. 2014;52(7):619-625. Published online June 28, 2014
- DOI: https://doi.org/10.1007/s12275-014-3479-4
- 54 View
- 0 Download
- 23 Crossref
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Abstract
- Bovine viral diarrhea virus (BVDV) is an enveloped, positive-sense, single-stranded RNA virus that belongs to the genus Pestivirus (Flaviviridae). The signaling pathways and levels of signaling molecules are altered in Madin-Darby Bovine Kidney (MDBK) cells infected with BVDV. Autophagy is a conservative biological degradation pathway that mainly eliminates and degrades damaged or superfluous organelles and macromolecular complexes for intracellular recycling in eukaryotic cells. Autophagy can also be induced as an effective response to maintain cellular homeostasis in response to different stresses, such as nutrient or growth factor deprivation, hypoxia, reactive oxygen species exposure and pathogen infection. However, the effects of BVDV infection on autophagy inMDBK cells remain unclear. Therefore, we performed an analysis of autophagic activity after BVDV NADL infection using real-time PCR, electron microscopy, laser confocal microscopy, and Western blotting analysis. The results demonstrated that BVDV NADL infection increased autophagic activity and significantly elevated the expression levels of the autophagy-related genes Beclin1 and ATG14 inMDBK cells. However, the knockdown of Beclin1 and ATG14 by RNA interference (RNAi) did not affect BVDV NADL infection-related autophagic activity. These findings provided a novel perspective to elaborate the effects of viral infection on the host cells.
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Citations
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Archives of Virology.2023;[Epub] CrossRef - In Vivo and In Vitro Antiviral Activity of Phlorizin Against Bovine Viral Diarrhea Virus
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Journal of Virology.2021;[Epub] CrossRef - Fate of the germ cells in mammalian ovary: A review
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