MSK
Search
- Page Path
- HOME > Search
Research Support, Non-U.S. Gov't
- Expression and Characterization of an Iron-Containing Superoxide Dismutase from Burkholderia pseudomallei
- Min-Hee Cho , Yong-Woo Shin , Jeong-Hoon Chun , Kee-Jong Hong , Byoung-Kuk Na , Gi-eun Rhie , Baik-Lin Seong , Cheon-Kwon Yoo
- J. Microbiol. 2012;50(6):1029-1033. Published online December 30, 2012
- DOI: https://doi.org/10.1007/s12275-012-2267-2
- 33 View
- 0 Download
- 6 Crossref
-
Abstract
- A superoxide dismutase (SOD) gene from Burkholderia pseudomallei, the causative agent of melioidosis, was cloned and expressed in Escherichia coli, and its product was functionally and physically characterized. The gene has an open-reading frame of 579 bp. The deduced amino acid sequence has 192 residues with a calculated molecular mass of ~22 kDa. Sequence comparison with other bacterial SODs showed that the protein contains typical metal-binding motifs and other Fe-SOD-conserved residues. The sequence has substantial similarity with other bacterial Fe-SOD sequences. The enzymatic activity of the expressed protein was inhibited by hydrogen peroxide but not by sodium azide or potassium cyanide, attributes that indeed are characteristic of typical bacterial Fe-SODs. Western blotting with antiserum against the recombinant Fe-SOD revealed that it is expressed in B. pseudomallei. Transformed E. coli that expressed the Fe-SOD had significantly increased SOD activity and was highly tolerant to paraquat-mediated replication inhibition, compared to transformed cells carrying an empty vector. Our results provide a basis for further biochemical characterization of the enzyme and elucidation of its role in the pathogenesis of B. pseudomallei.
-
Citations
Citations to this article as recorded by
- Identification of postharvest fruit biocontrol strain Burkholderia contaminans against fungi decay
Junfeng Shi, Jingting Du
European Journal of Horticultural Science.2023; 88(1): 1. CrossRef - Characterization of in vitro phenotypes of Burkholderia pseudomallei and Burkholderia mallei strains potentially associated with persistent infection in mice
R. C. Bernhards, C. K. Cote, K. Amemiya, D. M. Waag, C. P. Klimko, P. L. Worsham, S. L. Welkos
Archives of Microbiology.2017; 199(2): 277. CrossRef - Cloning and characterization of iron‐superoxide dismutase in Antarctic yeast strain Rhodotorula mucilaginosa AN5
Guangfeng Kan, Hua Wen, Xiaofei Wang, Ting Zhou, Cuijuan Shi
Journal of Basic Microbiology.2017; 57(8): 680. CrossRef - Cloning, expression and biochemical characterization of recombinant superoxide dismutase from Antarctic psychrophilic bacterium Pseudoalteromonas sp. ANT506
Quan‐Fu Wang, Yi‐Fan Wang, Yan‐Hua Hou, Yong‐Lei Shi, Han Han, Miao Miao, Ying‐Ying Wu, Yuan‐Ping Liu, Xiao‐Na Yue, Yu‐Jin Li
Journal of Basic Microbiology.2016; 56(7): 753. CrossRef - A predicted cation transporter protein, BPSS1228, is involved in intracellular behaviour ofBurkholderia pseudomalleiin a human lung epithelial cell line (A549)
Teerasit Techawiwattanaboon, Sorujsiri Chareonsudjai, Craig Winstanley
FEMS Microbiology Letters.2016; 363(23): fnw259. CrossRef - Physicochemical Properties Influencing Presence of Burkholderia pseudomallei in Soil from Small Ruminant Farms in Peninsular Malaysia
Hassan Ismail Musa, Latiffah Hassan, Zulkifli Hj. Shamsuddin, Chandrawathani Panchadcharam, Zunita Zakaria, Saleha Abdul Aziz, William C. Nierman
PLOS ONE.2016; 11(9): e0162348. CrossRef
- Identification of postharvest fruit biocontrol strain Burkholderia contaminans against fungi decay
First
Prev
TOP
