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- Impact of small RNA RaoN on nitrosative-oxidative stress resistance and virulence of Salmonella enterica serovar Typhimurium
- Sinyeon Kim , Yong Heon Lee
- J. Microbiol. 2020;58(6):499-506. Published online April 11, 2020
- DOI: https://doi.org/10.1007/s12275-020-0027-2
- 52 View
- 0 Download
- 5 Web of Science
- 6 Crossref
-
Abstract
-
RaoN is a Salmonella-specific small RNA that is encoded in
the cspH-envE intergenic region on Salmonella pathogenicity
island-11. We previously reported that RaoN is induced under
conditions of acid and oxidative stress combined with nutrient
limitation, contributing to the intramacrophage growth
of Salmonella enterica serovar Typhimurium. However, the
role of RaoN in nitrosative stress response and virulence has
not yet been elucidated. Here we show that the raoN mutant
strain has increased susceptibility to nitrosative stress by
using a nitric oxide generating acidified nitrite. Extending
previous research on the role of RaoN in oxidative stress resistance,
we found that NADPH oxidase inhibition restores
the growth of the raoN mutant in LPS-treated J774A.1 macrophages.
Flow cytometry analysis further revealed that the
inactivation of raoN leads to an increase in the intracellular
level of reactive oxygen species (ROS) in Salmonella-infected
macrophages, suggesting that RaoN is involved in the inhibition
of NADPH oxidase-mediated ROS production by mechanisms
not yet resolved. Moreover, we evaluated the effect
of raoN mutation on the virulence in murine systemic
infection and determined that the raoN mutant is less virulent
than the wild-type strain following oral inoculation. In
conclusion
, small regulatory RNA RaoN controls nitrosativeoxidative stress resistance and is required for virulence of Salmonella in mice. -
Citations
Citations to this article as recorded by
- The Salmonella enterica EnvE is an Outer Membrane Lipoprotein and Its Gene Expression Leads to Transcriptional Repression of the Virulence Gene msgA
Sinyeon Kim, Yong Heon Lee
Journal of Microbiology.2024; 62(11): 1013. CrossRef - Functions of Small Non-Coding RNAs in Salmonella–Host Interactions
Xia Meng, Mengping He, Pengpeng Xia, Jinqiu Wang, Heng Wang, Guoqiang Zhu
Biology.2022; 11(9): 1283. CrossRef - Detoxification Response of Pseudomonas fluorescens MFAF76a to Gaseous Pollutants NO2 and NO
Thibault Chautrand, Ségolène Depayras, Djouhar Souak, Mathilde Bouteiller, Tatiana Kondakova, Magalie Barreau, Mohamed Amine Ben Mlouka, Julie Hardouin, Yoan Konto-Ghiorghi, Sylvie Chevalier, Annabelle Merieau, Nicole Orange, Cécile Duclairoir-Poc
Microorganisms.2022; 10(8): 1576. CrossRef - Regulator of RNase E activity modulates the pathogenicity of Salmonella Typhimurium
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Microbial Pathogenesis.2022; 165: 105460. CrossRef - Gaseous NO2 induces various envelope alterations in Pseudomonas fluorescens MFAF76a
Thibault Chautrand, Ségolène Depayras, Djouhar Souak, Tatiana Kondakova, Magalie Barreau, Takfarinas Kentache, Julie Hardouin, Ali Tahrioui, Olivier Thoumire, Yoan Konto-Ghiorghi, Corinne Barbey, Guy Ladam, Sylvie Chevalier, Hermann J. Heipieper, Nicole O
Scientific Reports.2022;[Epub] CrossRef - Current challenges facing one-step production of l-ascorbic acid
Panpan Wang, Weizhu Zeng, Sha Xu, Guocheng Du, Jingwen Zhou, Jian Chen
Biotechnology Advances.2018; 36(7): 1882. CrossRef
- The Salmonella enterica EnvE is an Outer Membrane Lipoprotein and Its Gene Expression Leads to Transcriptional Repression of the Virulence Gene msgA
Research Support, Non-U.S. Gov't
- NOTE] Identification of Chaperones in Freeze Tolerance in Saccharomyces cerevisiae
- Mahendran Chinnamara Naicker , I Seul Jo , Hana Im
- J. Microbiol. 2012;50(5):882-887. Published online November 4, 2012
- DOI: https://doi.org/10.1007/s12275-012-2411-z
- 30 View
- 0 Download
- 10 Scopus
-
Abstract
- Exposure to low temperatures reduces protein folding rates and induces the cold denaturation of proteins. Considering the roles played by chaperones in facilitating protein folding and preventing protein aggregation, chaperones must exist that confer tolerance to cold stress. Here, yeast strains lacking individual chaperones were screened for reduced freezing tolerance. In total, 19 of 82 chaperone-deleted strains tested were more sensitive to freeze-thaw treatment than wild-type cells. The reintroduction of the respective chaperone genes into the deletion mutants recovered the freeze tolerance. The freeze sensitivity of the chaperone-knockout strains was also retained in the presence of 20% glycerol.
Retracted Publication
- NOTE] Identification of the Vibrio vulnificus htpG Gene and Its Influence on Cold Shock Recovery
- Slae Choi , Kyungku Jang , Seulah Choi , Hee-jee Yun , Dong-Hyun Kang
- J. Microbiol. 2012;50(4):707-711. Published online August 25, 2012
- DOI: https://doi.org/10.1007/s12275-012-2294-z
- 35 View
- 0 Download
- 9 Scopus
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Abstract
- An htpG gene encoding the heat shock protein HtpG was identified and cloned from Vibrio vulnificus. The deduced amino acid sequence of HtpG from V. vulnificus exhibited 71 and 85% identity to those reported from Escherichia coli and V. cholera, respectively. Functions of HtpG were assessed by the construction of an isogenic mutant whose htpG gene was deleted and by evaluating its phenotype changes during and after cold shock. The results demonstrated that recovery of the wild type from cold shock was significantly faster (p<0.05) than that of the htpG mutant, and indicated that the chaperone protein HtpG contributes to cold shock recovery, rather than cold shock tolerance, of V. vulnificus.
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