Journal of Microbiology

Journal Articles

Role of putative virulence traits of Campylobacter jejuni in regulating differential host immune responses: Ankita Singh , Amirul Islam Mallick; J. Microbiol. 2019;57(4):298-309. Published online February 22, 2019; DOI: https://doi.org/10.1007/s12275-019-8165-0

54 View
0 Download
13 Web of Science
10 Crossref

Among the major enteric pathogens, Campylobacter jejuni is considered an important source of diarrheal illness in humans. In contrast to the acute gastroenteritis in humans, C. jejuni exhibits prolonged cecal colonization at a high level with little or no pathology in chickens. Although several known virulence determinants of C. jejuni have been found to be associated with a higher degree of pathogenesis in humans, to date, little is known about their functions in the persistent colonization of chickens. The present study was undertaken to assess the role of C. jejuni in imparting differential host immune responses in human and chicken cells. Based on the abundance of major genes encoding virulence factors (GEVFs), we used a particular isolate that harbors the cadF, flaA, peb1, racR, ciaB, cdtB, and hcp genes. This study showed that hypervirulent C. jejuni isolate that encodes a functional type VI secretion system (T6SS) has a greater ability to invade and create characteristic “attaching and effacing” lesions in human INT407 compared to primary chicken embryo intestinal cells (CEICs). Furthermore, we demonstrated that the higher bacterial invasion in human INT407 triggered higher levels of expression of major proinflammatory cytokines, such as IL- 1β and IL-6, and significant downregulation of IL-17A gene expression (P ≤ 0.05). The findings of the present study suggest that the enhanced ability of C. jejuni to invade human cells is tightly regulated by proinflammatory cytokines in the gut and possibly holds the keys to the observed differences in pathogenesis between human and chicken cells.

Citations

Citations to this article as recorded by

Targeted Bioimaging of Microencapsulated Recombinant LAB Vector Expressing Fluorescent Reporter Protein: A Non-invasive Approach for Microbial Tracking
Prakash Biswas, Afruja Khan, Amirul Islam Mallick
ACS Biomaterials Science & Engineering.2024; 10(8): 5210. CrossRef
Identification and functional characterization of putative ligand binding domain(s) of JlpA protein of Campylobacter jejuni
Chandan Gorain, Subhadeep Gupta, S.S. Mahafujul Alam, Mehboob Hoque, Andrey V. Karlyshev, Amirul Islam Mallick
International Journal of Biological Macromolecules.2024; 264: 130388. CrossRef
Heterogeneity and Compositional Diversities of Campylobacter jejuni Outer Membrane Vesicles (OMVs) Drive Multiple Cellular Uptake Processes
Afruja Khan, Avijit Sardar, Pradip K. Tarafdar, Amirul I. Mallick
ACS Infectious Diseases.2023; 9(11): 2325. CrossRef
Multimodal Biofilm Inactivation Using a Photocatalytic Bismuth Perovskite–TiO2–Ru(II)polypyridyl-Based Multisite Heterojunction
Noufal Kandoth, Sonu Pratap Chaudhary, Subhadeep Gupta, Kumari Raksha, Atin Chatterjee, Shresth Gupta, Safakath Karuthedath, Catherine S. P. De Castro, Frédéric Laquai, Sumit Kumar Pramanik, Sayan Bhattacharyya, Amirul Islam Mallick, Amitava Das
ACS Nano.2023; 17(11): 10393. CrossRef
In Silico and In Vitro Analysis of Helicobacter pullorum Type Six Secretory Protein Hcp and Its Role in Bacterial Invasion and Pathogenesis
Kashaf Javed, Farzana Gul, Rashda Abbasi, Sidra Batool, Zobia Noreen, Habib Bokhari, Sundus Javed
Current Microbiology.2022;[Epub] CrossRef
Gut Microbe-Derived Outer Membrane Vesicles: A Potential Platform to Control Cecal Load of Campylobacter jejuni
Ankita Singh, Afruja Khan, Tamal Ghosh, Samiran Mondal, Amirul I. Mallick
ACS Infectious Diseases.2021; 7(5): 1186. CrossRef
Mucosal delivery of live Lactococcus lactis expressing functionally active JlpA antigen induces potent local immune response and prevent enteric colonization of Campylobacter jejuni in chickens
Chandan Gorain, Ankita Singh, Sudipta Bhattacharyya, Anirban Kundu, Aritraa Lahiri, Subhadeep Gupta, Amirul I. Mallick
Vaccine.2020; 38(7): 1630. CrossRef
Immunopathological properties of the Campylobacter jejuni flagellins and the adhesin CadF as assessed in a clinical murine infection model
Anna-Maria Schmidt, Ulrike Escher, Soraya Mousavi, Nicole Tegtmeyer, Manja Boehm, Steffen Backert, Stefan Bereswill, Markus M. Heimesaat
Gut Pathogens.2019;[Epub] CrossRef
A One Health approach to prevention, treatment, and control of campylobacteriosis
Francesca Schiaffino, James Platts-Mills, Margaret N. Kosek
Current Opinion in Infectious Diseases.2019; 32(5): 453. CrossRef
Immunogenicity and protective efficacy of mucosal delivery of recombinant hcp of Campylobacter jejuni Type VI secretion system (T6SS) in chickens
Ankita Singh, Khairun Nisaa, Sudipta Bhattacharyya, Amirul Islam Mallick
Molecular Immunology.2019; 111: 182. CrossRef

Epidemiological relationships of Campylobacter jejuni strains isolated from humans and chickens in South Korea: Jae-Young Oh , Yong-Kuk Kwon , Bai Wei , Hyung-Kwan Jang , Suk-Kyung Lim , Cheon-Hyeon Kim , Suk-Chan Jung , Min-Su Kang; J. Microbiol. 2017;55(1):13-20. Published online December 30, 2016; DOI: https://doi.org/10.1007/s12275-017-6308-8

59 View
0 Download
30 Crossref

Abstract

Thirty-nine human isolates of Campylobacter jejuni obtained from a national university hospital during 2007–2010 and 38 chicken isolates of C. jejuni were collected from poultry farms during 2009–2010 in South Korea were used in this study. Campylobacter genomic species and virulence-associated genes were identified by PCR. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were performed to compare their genetic relationships. All isolates were highly resistant to ciprofloxacin, nalidixic acid, and tetracycline. Of all isolates tested, over 94% contained seven virulence associated genes (flaA, cadF, racR, dnaJ, cdtA, cdtB, and cdtC). All isolates were classified into 39 types by PFGE clustering with 90% similarity. Some chicken isolates were incorporated into some PFGE types of human isolates. MLST analysis for the 39 human isolates and 38 chicken isolates
result
ed in 14 and 23 sequence types (STs), respectively, of which 10 STs were new. STs overlapped in both chicken and human isolates included ST-21, ST-48, ST-50, ST-51, and ST-354, of which ST-21 was the predominant ST in both human and chicken isolates. Through combined analysis of PFGE types and STs, three chicken isolates were clonally related to the three human isolates associated with food poisoning (VII-ST-48, XXII-ST-354, and XXVIII-ST-51). They were derived from geographically same or distinct districts. Remarkably, clonal spread of food poisoning pathogens between animals and humans was confirmed by population genetic analysis. Consequently, contamination of campylobacters with quinolone resistance and potential virulence genes in poultry production and consumption may increase the risk of infections in humans.

Citations

Citations to this article as recorded by

Trends, clinical characteristics, antimicrobial susceptibility patterns, and outcomes of Campylobacter bacteraemia: a multicentre retrospective study
Yae Jee Baek, Je Eun Song, Eun Jin Kim, Heun Choi, Yujin Sohn, Yong Duk Jeon, Eun Hwa Lee, Jin Young Ahn, Su Jin Jeong, Nam Su Ku, Jun Yong Choi, Joon-Sup Yeom, Young Goo Song, Jung Ho Kim
Infection.2024; 52(3): 857. CrossRef
Molecular Detection of Virulence‐Associated Markers in Campylobacter coli and Campylobacter jejuni Isolates From Water, Cattle, and Chicken Faecal Samples From Kajiado County, Kenya
Daniel W. Wanja, Christine M. Mbindyo, Paul G. Mbuthia, Lilly C. Bebora, Gabriel O. Aboge, Kalman Imre
BioMed Research International.2024;[Epub] CrossRef
Epidemiological and Genomic Characterization of a Campylobacter jejuni Outbreak in Lishui, China
Yumei Ge, Youqi Ji, Jianhua Mei, Maojun Zhang, Yumin Li, Bifeng Ye, Honghu Chen, Xiuying Chen
Foodborne Pathogens and Disease.2024;[Epub] CrossRef
Virulence Genes, Antimicrobial Resistance, and Genotypes of Campylobacter jejuni Isolated from Chicken Slaughterhouses in South Korea
Jiyeon Jeong, Ji-Youn Lee, Jin-San Moon, Min-Su Kang, Sung-Il Kang, O-Mi Lee, So-Hee Lee, Yong-Kuk Kwon, Myeongju Chae, Seongbeom Cho
Foodborne Pathogens and Disease.2024;[Epub] CrossRef
Antimicrobial Susceptibility Patterns and Genetic Diversity of Campylobacter spp. Isolates from Patients with Diarrhea in South Korea
So Yeon Kim, Dongheui An, Hyemi Jeong, Jonghyun Kim
Microorganisms.2024; 12(1): 94. CrossRef
Campylobacterspp. isolated from poultry in Iran: Antibiotic resistance profiles, virulence genes, and molecular mechanisms
Seyedeh Bita Mousavinafchi, Ebrahim Rahimi, Amir Shakerian
Food Science & Nutrition.2023; 11(2): 1142. CrossRef
Persistence of Campylobacter spp. in Poultry Flocks after Disinfection, Virulence, and Antimicrobial Resistance Traits of Recovered Isolates
Manel Gharbi, Awatef Béjaoui, Safa Hamrouni, Amel Arfaoui, Abderrazak Maaroufi
Antibiotics.2023; 12(5): 890. CrossRef
Relationships between Virulence Genes and Antibiotic Resistance Phenotypes/Genotypes in Campylobacter spp. Isolated from Layer Hens and Eggs in the North of Tunisia: Statistical and Computational Insights
Manel Gharbi, Selim Kamoun, Chaima Hkimi, Kais Ghedira, Awatef Béjaoui, Abderrazak Maaroufi
Foods.2022; 11(22): 3554. CrossRef
Prevalence and Antimicrobial Susceptibility of Campylobacter Species with Particular Focus on the Growth Promoting, Immunostimulant and Anti-Campylobacter jejuni Activities of Eugenol and Trans-Cinnamaldehyde Mixture in Broiler Chickens
Ahmed Aljazzar, Marwa I. Abd El-Hamid, Rania M. S. El-Malt, Waleed Rizk El-Gharreb, Sherief M. Abdel-Raheem, Abdelazim M. Ibrahim, Adel M. Abdelaziz, Doaa Ibrahim
Animals.2022; 12(7): 905. CrossRef
Genotypical Relationship Between Human and Poultry Strains of Campylobacter jejuni
Roberta Torres de Melo, Carolyne Ferreira Dumont, Raquelline Figueiredo Braz, Guilherme Paz Monteiro, Micaela Guidotti Takeuchi, Eduarda Cristina Alves Lourenzatto, Jandra Pacheco dos Santos, Daise Aparecida Rossi
Current Microbiology.2021; 78(8): 2980. CrossRef
Genomic Characterization of Fluoroquinolone-Resistant Thermophilic Campylobacter Strains Isolated from Layer Chicken Feces in Gangneung, South Korea by Whole-Genome Sequencing
Noel Gahamanyi, Dae-Geun Song, Kye-Yoon Yoon, Leonard E. G. Mboera, Mecky I. Matee, Dieudonné Mutangana, Erick V. G. Komba, Cheol-Ho Pan, Raghavendra G. Amachawadi
Genes.2021; 12(8): 1131. CrossRef
Genomic Relatedness, Antibiotic Resistance and Virulence Traits of Campylobacter jejuni HS19 Isolates From Cattle in China Indicate Pathogenic Potential
Xiaoqi Zang, Pingyu Huang, Jie Li, Xinan Jiao, Jinlin Huang
Frontiers in Microbiology.2021;[Epub] CrossRef
DNA sequencing, genomes and genetic markers of microbes on fruits and vegetables
Youming Shen, Jiyun Nie, Lixue Kuang, Jianyi Zhang, Haifei Li
Microbial Biotechnology.2021; 14(2): 323. CrossRef
Longitudinal Study of the Distribution of Antimicrobial-Resistant Campylobacter Isolates from an Integrated Broiler Chicken Operation
Bo-Ram Kwon, Bai Wei, Se-Yeoun Cha, Ke Shang, Jun-Feng Zhang, Min Kang, Hyung-Kwan Jang
Animals.2021; 11(2): 246. CrossRef
Review on Stress Tolerance in Campylobacter jejuni
Se-Hun Kim, Ramachandran Chelliah, Sudha Rani Ramakrishnan, Ayyappasamy Sudalaiyadum Perumal, Woo-Suk Bang, Momna Rubab, Eric Banan-Mwine Daliri, Kaliyan Barathikannan, Fazle Elahi, Eunji Park, Hyeon Yeong Jo, Su-Bin Hwang, Deog Hwan Oh
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Antimicrobial Resistance and PFGE Molecular Typing of Salmonella enterica serovar Gallinarum Isolates from Chickens in South Korea from 2013 to 2018
Jun-Feng Zhang, Ke Shang, Jong-Yeol Park, Yea-Jin Lee, Yu-Ri Choi, Sang-Won Kim, Se-Yeoun Cha, Hyung-Kwan Jang, Bai Wei, Min Kang
Animals.2021; 12(1): 83. CrossRef
Antimicrobial Resistance Profiles, Virulence Genes, and Genetic Diversity of Thermophilic Campylobacter Species Isolated From a Layer Poultry Farm in Korea
Noel Gahamanyi, Dae-Geun Song, Kye-Yoon Yoon, Leonard E. G. Mboera, Mecky I. Matee, Dieudonné Mutangana, Raghavendra G. Amachawadi, Erick V. G. Komba, Cheol-Ho Pan
Frontiers in Microbiology.2021;[Epub] CrossRef
Prevalence, Antimicrobial Susceptibility, Virulence and Genotyping of Campylobacter jejuni with a Special Reference to the Anti-Virulence Potential of Eugenol and Beta-Resorcylic Acid on Some Multi-Drug Resistant Isolates in Egypt
Ahmed M. Ammar, El-Sayed Y. El-Naenaeey, Rania M. S. El-Malt, Attia A. El-Gedawy, Eman Khalifa, Shimaa S. Elnahriry, Marwa I. Abd El-Hamid
Animals.2020; 11(1): 3. CrossRef
Epidemiological and Whole Genomic Sequencing Analysis of a Campylobacter jejuni Outbreak in Zhejiang Province, China, May 2019
Honghu Chen, Yaxin Dai, Jiancai Chen, Yunyi Zhang, Li Zhan, Lingling Mei, Hongling Wang
Foodborne Pathogens and Disease.2020; 17(12): 775. CrossRef
Molecular Characterization and Antibiotic Resistant Profiles of Campylobacter Species Isolated From Poultry and Diarrheal Patients in Southeastern China 2017–2019
Leyi Zhang, Yi Li, Yongqiang Shao, Yuqin Hu, Huihuang Lou, Xiaonan Chen, Yuejin Wu, Lingling Mei, Biao Zhou, Xibin Zhang, Wenwu Yao, Lei Fang, Yanjun Zhang
Frontiers in Microbiology.2020;[Epub] CrossRef
Comparative restriction enzyme mapping of Campylobacter jejuni isolates from turkeys and broilers based on flaA flagellar gene using HpyF3I endonuclease
Elham Atefi Tabar, Hamid Staji, Ali Mahdavi
Folia Microbiologica.2019; 64(2): 189. CrossRef
Genetic Diversity of Campylobacter jejuni Isolated From Avian and Human Sources in Egypt
Marwa I. Abd El-Hamid, Norhan K. Abd El-Aziz, Mohamed Samir, El-sayed Y. El-Naenaeey, Etab M. Abo Remela, Rasha A. Mosbah, Mahmoud M. Bendary
Frontiers in Microbiology.2019;[Epub] CrossRef
Prevalence, antimicrobial resistance profiles and virulence-associated genes of thermophilic Campylobacter spp. isolated from ducks in a Chinese slaughterhouse
Xinfeng Han, Xingai Guan, Hang Zeng, Jiakang Li, Xuelin Huang, Yiping Wen, Qin Zhao, Xiaobo Huang, Qigui Yan, Yong Huang, Sanjie Cao, Rui Wu, Xiaoping Ma, Likou Zou
Food Control.2019; 104: 157. CrossRef
Clinical and microbiological characteristics of patients with bacteremia caused by Campylobacter species with an emphasis on the subspecies of C. fetus
Yen-Hung Liu, Wataru Yamazaki, Yu-Tsung Huang, Chun-Hsing Liao, Wang-Hui Sheng, Po-Ren Hsueh
Journal of Microbiology, Immunology and Infection.2019; 52(1): 122. CrossRef
Source attribution of Campylobacter jejuni shows variable importance of chicken and ruminants reservoirs in non-invasive and invasive French clinical isolates
Elvire Berthenet, Amandine Thépault, Marianne Chemaly, Katell Rivoal, Astrid Ducournau, Alice Buissonnière, Lucie Bénéjat, Emilie Bessède, Francis Mégraud, Samuel K. Sheppard, Philippe Lehours
Scientific Reports.2019;[Epub] CrossRef
A Review of the Effect of Management Practices on Campylobacter Prevalence in Poultry Farms
Nompilo Sibanda, Aaron McKenna, Anne Richmond, Steven C. Ricke, Todd Callaway, Alexandros Ch. Stratakos, Ozan Gundogdu, Nicolae Corcionivoschi
Frontiers in Microbiology.2018;[Epub] CrossRef
The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2016

EFSA Journal.2018;[Epub] CrossRef
Comparative clustering and genotyping of Campylobacter jejuni strains isolated from broiler and turkey feces by using RAPD-PCR and ERIC-PCR analysis
Hamid Staji, Soghra Farhani Birgani, Behnaz Raeisian
Annals of Microbiology.2018; 68(11): 755. CrossRef
Antibiotic Susceptibility, Genetic Diversity, and the Presence of Toxin Producing Genes in Campylobacter Isolates from Poultry
Jeeyeon Lee, Jiyeon Jeong, Heeyoung Lee, Jimyeong Ha, Sejeong Kim, Yukyung Choi, Hyemin Oh, Kunho Seo, Yohan Yoon, Soomin Lee
International Journal of Environmental Research and Public Health.2017; 14(11): 1400. CrossRef
Molecular Detection, Typing, and Quantification of Campylobacter spp. in Foods of Animal Origin
Beatriz da Silva Frasao, Victor Augustus Marin, Carlos Adam Conte‐Junior
Comprehensive Reviews in Food Science and Food Safety.2017; 16(4): 721. CrossRef

First
Prev
Page of 1
Next
Last

Search