Journal of Microbiology

Journal Article

Lytic KFS-SE2 phage as a novel bio-receptor for Salmonella Enteritidis detection: In Young Choi , Cheonghoon Lee , Won Keun Song , Sung Jae Jang , Mi-Kyung Park; J. Microbiol. 2019;57(2):170-179. Published online January 31, 2019; DOI: https://doi.org/10.1007/s12275-019-8610-0

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Since Salmonella Enteritidis is one of the major foodborne pathogens, on-site applicable rapid detection methods have been required for its control. The purpose of this study was to isolate and purify S. Enteritidis-specific phage (KFS-SE2 phage) from an eel farm and to investigate its feasibility as a novel, efficient, and reliable bio-receptor for its employment. KFS-SE2 phage was successfully isolated at a high concentration of (2.31 ± 0.43) × 1011 PFU/ml, and consisted of an icosahedral head of 65.44 ± 10.08 nm with a non-contractile tail of 135.21 ± 12.41 nm. The morphological and phylogenetic analysis confirmed that it belongs to the Pis4avirus genus in the family of Siphoviridae. KFS-SE2 genome consisted of 48,608 bp with 45.7% of GC content. Genome analysis represented KFS-SE2 to have distinctive characteristics as a novel phage. Comparative analysis of KFS-SE2 phage with closely related strains confirmed its novelty by the presence of unique proteins. KFS-SE2 phage exhibited excellent specificity to S. Enteritidis and was stable under the temperature range of 4 to 50°C and pH of 3 to 11 (P < 0.05). The latent time was determined to be 20 min. Overall, a new lytic KFS-SE2 phage was successfully isolated from the environment at a high concentration and the excellent feasibility of KFS-SE2 phage was demonstrated as a new bio-receptor for S. Enteritidis detection.

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Detection of Cholera Cells Using Surface Plasmon Resonance Sensor: Choi, Ki Bong , Youn, Hee Ju , Ha, Youn Chul , Kim, Kwang Joong , Choi, Jung Do; J. Microbiol. 1998;36(1):43-48.

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Abstract: In this report, we describe the application of colloidal gold particles for bacterial cell detection using a surface plasmon resonance(SPR) sensor. A monoclonal antibody against V. cholere was immobilized onto the gold film of a sensor chip activated with N-hydroxysuccinimide. Optimal conditions for the immunochemical reaction for cholera detection was investigated with streptavidin coated colloidal fold particles and ciotinylated monoclonal antibody against cholera cells. The sensitivity of the cholera cell assay with SPR sensor could be enhanced 1000 times by binding of anti-cholera monoclonal antibody-biotin conjugate and streptavidin coated colloidal gold suspension. From a viewpoint of sensitivity, SPR sensor is about 10 times more sensitive than ELISA. This result illustrates the feasibility of using the SPR sensor in bacterial cell detection.

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