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- Descr!ption of Ornithinimicrobium cryptoxanthini sp. nov., a Novel Actinomycete Producing β‑cryptoxanthin Isolated from the Tongtian River Sediments
- Yuyuan Huang , Yifan Jiao , Sihui Zhang , Yuanmeihui Tao , Suping Zhang , Dong Jin , Ji Pu , Liyun Liu , Jing Yang , Shan Lu
- J. Microbiol. 2023;61(4):379-388. Published online March 16, 2023
- DOI: https://doi.org/10.1007/s12275-023-00029-5
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Abstract
- Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37′13″N, 96°05′37″E) in July 2019. Both strains were shown to grow at 15–35 °C and pH 7.0–10.0, and in the presence of 0–6.0% (w/v) NaCl. The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6–98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5–98.6%) and O. murale 01-Gi-040T (98.3–98.5%). The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0–23.9% and 70.8–80.4%, respectively, all below the respective recommended 70.0% and 95–96% cutoff point. Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9. Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids. β-cryptoxanthin ( C40H56O) can be extracted from strain JY.X270T, and its content is 6.3 μg/ml. Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp. nov. is proposed (type strain JY.X270T = CGMCC 1.19147T = JCM 34882T).
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Citations
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- Screening, identification, and characterization of high potential bacteria for ꞵ-cryptoxanthin production from natural sources
Sopida Korkerd, Savitri Vatanyoopaisarn, Wonnop Visessaguan, Benjawan Thumthanarak, Dudsadee Uttapap, Solange I. Mussatto, Vilai Rungsardthong
Biocatalysis and Agricultural Biotechnology.2024; 57: 103089. CrossRef
- Screening, identification, and characterization of high potential bacteria for ꞵ-cryptoxanthin production from natural sources
- Bacillus ferrooxidans sp. nov., an iron(II)-oxidizing bacterium isolated from paddy soil
- Guo-Wei Zhou , Xiao-Ru Yang , Jian-Qiang Su , Bang-Xiao Zheng , Yong-Guan Zhu
- J. Microbiol. 2018;56(7):472-477. Published online June 14, 2018
- DOI: https://doi.org/10.1007/s12275-018-7543-3
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- 4 Crossref
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Abstract
- An endospore-forming bacterium, designated YT-3T, was isolated from a paddy soil in Yingtan, Jiangxi, China. Cells of strain YT-3T were Gram-positive, rod-shaped, facultative anaerobic, catalase, and oxidase positive. The optimum growth temperature and pH were 30°C (ranged from 15 to 50°C) and 6.5–7.0 (ranged from 3 to 11), respectively. Analysis of the 16S rRNA gene sequence showed that strain YT-3T was affiliated to the genus Bacillus and displayed the highest similarity to that of Bacillus drentensis JCM 21707T (98.3%), followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli JCM 21708T (97.0%). The similarity of rpoB gene sequence between strain YT-3T and B. drentensis JCM 21707T, B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was 80.4%, 81.5%, and 82.1%, respectively. The genomic DNA G + C content was 44.9 mol%. The predominant respiratory quinone was Menaquinone-7, and meso-diaminopimelic acid was present in the peptidoglycan layer of cell wall. The major fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%), C15:0 iso (17.4%), and C16:0 iso (9.8%). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipids, and ammoniac phospholipids. The DNA-DNA hybridization values between isolate YT-3T and B. drentensis (JCM 21707T), B. ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T) were 36.3%, 30.3%, and 25.3%, respectively. On the basis of physiological, genetic and biochemical data, strain YT-3T represented a novel species of the genus Bacillus, for which the name Bacillus ferrooxidans sp. nov was proposed. The type strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T).
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Citations
Citations to this article as recorded by- Discarded masks as hotspots of antibiotic resistance genes during COVID-19 pandemic
Shu-Yi-Dan Zhou, Chenshuo Lin, Kai Yang, Le-Yang Yang, Xiao-Ru Yang, Fu-Yi Huang, Roy Neilson, Jian-Qiang Su, Yong-Guan Zhu
Journal of Hazardous Materials.2022; 425: 127774. CrossRef - Underestimation about the Contribution of Nitrate Reducers to Iron Cycling Indicated by Enterobacter Strain
Ming-Jun Li, Meng-Yun Wei, Xiao-Ting Fan, Guo-Wei Zhou
Molecules.2022; 27(17): 5581. CrossRef - Fe toxicity in plants: Impacts and remediation
Noreen Zahra, Muhammad Bilal Hafeez, Kanval Shaukat, Abdul Wahid, Mirza Hasanuzzaman
Physiologia Plantarum.2021;[Epub] CrossRef - Metabolic Inactivity and Re-awakening of a Nitrate Reduction Dependent Iron(II)-Oxidizing Bacterium Bacillus ferrooxidans
Guo-Wei Zhou, Xiao-Ru Yang, Regin Rønn, Jian-Qiang Su, Li Cui, Bang-Xiao Zheng, Yong-Guan Zhu
Frontiers in Microbiology.2019;[Epub] CrossRef
- Discarded masks as hotspots of antibiotic resistance genes during COVID-19 pandemic
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