Journal of Microbiology

Reviews

Understanding the Diversity and Roles of the Ruminal Microbiome: Gi Beom Keum, Sriniwas Pandey, Eun Sol Kim, Hyunok Doo, Jinok Kwak, Sumin Ryu, Yejin Choi, Juyoun Kang, Sheena Kim, Hyeun Bum Kim; J. Microbiol. 2024;62(3):217-230. Published online April 25, 2024; DOI: https://doi.org/10.1007/s12275-024-00121-4

178 View
20 Download
11 Web of Science
14 Crossref

The importance of ruminal microbiota in ruminants is emphasized, not only as a special symbiotic relationship with ruminants but also as an interactive and dynamic ecosystem established by the metabolites of various rumen microorganisms. Rumen microbial community is essential for life maintenance and production as they help decompose and utilize fber that is difcult to digest, supplying about 70% of the energy needed by the host and 60–85% of the amino acids that reach the small intestine. Bacteria are the most abundant in the rumen, but protozoa, which are relatively large, account for 40–50% of the total microorganisms. However, the composition of these ruminal microbiota is not conserved or constant throughout life and is greatly infuenced by the host. It is known that the initial colonization of calves immediately after birth is mainly infuenced by the mother, and later changes depending on various factors such as diet, age, gender and breed. The initial rumen microbial community contains aerobic and facultative anaerobic bacteria due to the presence of oxygen, but as age increases, a hypoxic environment is created inside the rumen, and anaerobic bacteria become dominant in the rumen microbial community. As calves grow, taxonomic diversity increases, especially as they begin to consume solid food. Understanding the factors afecting the rumen microbial community and their efects and changes can lead to the early development and stabilization of the microbial community through the control of rumen microorganisms, and is expected to ultimately help improve host productivity and efciency.

Citations

Citations to this article as recorded by

The microbiome’s influence on obesity: mechanisms and therapeutic potential
Dawit Adisu Tadese, James Mwangi, Lei Luo, Hao Zhang, Xiaoshan Huang, Brenda B. Michira, Shengwen Zhou, Peter Muiruri Kamau, Qiumin Lu, Ren Lai
Science China Life Sciences.2025; 68(3): 657. CrossRef
Mitigating enteric methane emissions: An overview of methanogenesis, inhibitors and future prospects
Xin Xie, Yurong Cao, Qiushuang Li, Qi Li, Xingze Yang, Rong Wang, Xiumin Zhang, Zhiliang Tan, Bo Lin, Min Wang
Animal Nutrition.2025;[Epub] CrossRef
Stochastic and deterministic factors that shape the rumen microbiome
Samodha C. Fernando, Seidu Adams, Andrew Lakamp, Matthew L. Spangler
Journal of Dairy Science.2025;[Epub] CrossRef
Genome analysis of Lactococcus taiwanensis strain K_LL001 with potential cellulose degrading functions
Eun Sol Kim, Jin Ho Cho, Minho Song, Sheena Kim, Gi Beom Keum, Hyunok Doo, Jinok Kwak, Sriniwas Pandey, Sumin Ryu, Yejin Choi, Juyoun Kang, Hyeun Bum Kim, Ju-Hoon Lee
Journal of Animal Science and Technology.2025; 67(1): 273. CrossRef
Dynamic Changes in Rumen Microbial Diversity and Community Composition Within Rumen Fluid in Response to Various Storage Temperatures and Preservation Times
Chang Liu, Jin Cheng, Yunong Xie, Kehui Ouyang, Mingren Qu, Ke Pan, Qinghua Qiu
Veterinary Sciences.2025; 12(3): 234. CrossRef
Integrated multi-omics to elucidate the interplay between rumen microorganisms and host metabolism in Hu sheep supplemented with herbal preparations
Chunhui Wang, Qiao Li, Xingcai Qi, Huihui Wang, Yi Wu, Keyan Ma, Juanjuan Song, Zilong Liu, Youji Ma, Garret Suen
mSphere.2025;[Epub] CrossRef
Investigation on the action mechanisms of taurine on rumen microbial protein synthesis and nitrogen metabolism in beef steers using sodium sulfate as a contrast
Manman Fan, Jinming Hu, Cheng Liu, Shuo Zhang, Yufeng Liu, Guangyong Zhao
Animal Nutrition.2025;[Epub] CrossRef
Effects of Oregano Essential Oil and/or Yeast Cultures on the Rumen Microbiota of Crossbred Simmental Calves
Ting Liu, Zhihao Luo, Tao Zhang, Huan Chen, Xuejiao Yi, Jiang Hu, Bingang Shi, Yuxi An, Changze Cui, Xiangyan Wang
Animals.2024; 14(24): 3710. CrossRef
Host-Associated Microbiome
Woo Jun Sul
Journal of Microbiology.2024; 62(3): 135. CrossRef
GnRH Immunocastration in Male Xizang Sheep: Impacts on Rumen Microbiome and Metabolite Profiles for Enhanced Health and Productivity
Xiaoming Zhang, Tianzeng Song, Guiqiong Liu, Jing Wu, Yangzong Zhaxi, Shehr Bano Mustafa, Khuram Shahzad, Xiaoying Chen, Wangsheng Zhao, Xunping Jiang
Animals.2024; 14(20): 2942. CrossRef
Gut Akkermansia muciniphila, Prevotellaceae, and Enterobacteriaceae spp. as Possible Markers in Women-Related Nutritional and Clinical Trials: Familial Mediterranean Fever Disease
Astghik Pepoyan
Women's Health Reports.2024; 5(1): 785. CrossRef
Exploring the Spatial Variation in the Microbiota and Bile Acid Metabolism of the Compound Stomach in Intensively Farmed Yaks
Shichun He, Zaimei Yuan, Sifan Dai, Zibei Wang, Shusheng Zhao, Bin Zhang, Huaming Mao, Dongwang Wu
Microorganisms.2024; 12(10): 1968. CrossRef
Investigation of the impact of multi-strain probiotics containing Saccharomyces cerevisiae on porcine production
Sheena Kim, Jinho Cho, Gi Beom Keum, Jinok Kwak, Hyunok Doo, Yejin Choi, Juyoun Kang, Haram Kim, Yeongjae Chae, Eun Sol Kim, Minho Song, Hyeun Bum Kim
Journal of Animal Science and Technology.2024; 66(5): 876. CrossRef
The Effects of Mixed Inoculum Storage Time on In Vitro Rumen Fermentation Characteristics, Microbial Diversity, and Community Composition
Chang Liu, Jing Ge, Jiaqi Dai, Mingren Qu, Kehui Ouyang, Qinghua Qiu
Animals.2024; 15(1): 5. CrossRef

Genomic Evolution and Recombination Dynamics of Human Adenovirus D Species: Insights from Comprehensive Bioinformatic Analysis: Anyeseu Park, Chanhee Lee, Jeong Yoon Lee; J. Microbiol. 2024;62(5):393-407. Published online March 7, 2024; DOI: https://doi.org/10.1007/s12275-024-00112-5

72 View
0 Download
1 Web of Science
1 Crossref

Abstract

Human adenoviruses (HAdVs) can infect various epithelial mucosal cells, ultimately causing different symptoms in infected organ systems. With more than 110 types classified into seven species (A-G), HAdV-D species possess the highest number of viruses and are the fastest proliferating. The emergence of new adenovirus types and increased diversity are driven by homologous recombination (HR) between viral genes, primarily in structural elements such as the penton base, hexon and fiber proteins, and the E1 and E3 regions. A comprehensive analysis of the HAdV genome provides valuable insights into the evolution of human adenoviruses and identifies genes that display high variation across the entire genome to determine recombination patterns. Hypervariable regions within genetic sequences correlate with functional characteristics, thus allowing for adaptation to new environments and hosts. Proteotyping of newly emerging and already established adenoviruses allows for prediction of the characteristics of novel viruses. HAdV-D species evolved in a direction that increased diversity through gene recombination. Bioinformatics analysis across the genome, particularly in highly variable regions, allows for the verification or re-evaluation of recombination patterns in both newly introduced and pre-existing viruses, ultimately aiding in tracing various biological traits such as virus tropism and pathogenesis. Our research does not only assist in predicting the emergence of new adenoviruses but also offers critical guidance in regard to identifying potential regulatory factors of homologous recombination hotspots.

Citations

Citations to this article as recorded by

In Silico Intensive Analysis for the E4 Gene Evolution of Human Adenovirus Species D
Chanhee Lee, Anyeseu Park, Jeong Yoon Lee
Journal of Microbiology.2024; 62(5): 409. CrossRef

Journal Articles

Effects of Feather Hydrolysates Generated by Probiotic Bacillus licheniformis WHU on Gut Microbiota of Broiler and Common carp: Kamin Ke, Yingjie Sun, Tingting He, Wenbo Liu, Yijiao Wen, Siyuan Liu, Qin Wang, Xiaowei Gao; J. Microbiol. 2024;62(6):473-487. Published online February 29, 2024; DOI: https://doi.org/10.1007/s12275-024-00118-z

60 View
0 Download
2 Web of Science
1 Crossref

Abstract

Due to the ever-increasing demand for meat, it has become necessary to identify cheap and sustainable sources of protein for animal feed. Feathers are the major byproduct of poultry industry, which are rich in hard-to-degrade keratin protein. Previously we found that intact feathers can be digested into free amino acids, short peptides, and nano-/micro-keratin particles by the strain Bacillus licheniformis WHU in water, and the resulting feather hydrolysates exhibit prebiotic effects on mice. To explore the potential utilization of feather hydrolysate in the feed industry, we investigated its effects on the gut microbiota of broilers and fish. Our results suggest that feather hydrolysates significantly decrease and increase the diversity of gut microbial communities in broilers and fish, respectively. The composition of the gut microbiota was markedly altered in both of the animals. The abundance of bacteria with potentially pathogenic phenotypes in the gut microbial community of the fish significantly decreased. Staphylococcus spp., Pseudomonas spp., Neisseria spp., Achromobacter spp. were significantly inhibited by the feather hydrolysates. In addition, feather hydrolysates significantly improved proteolytic activity in the guts of broilers and fish. In fish, the expression levels of ZO-1 and TGF-α significantly improved after administration of feather hydrolysates. The results presented here suggest that feather hydrolysates generated by B. licheniformis WHU could be an alternative protein source in aquaculture and could exert beneficial effects on fish.

Citations

Citations to this article as recorded by

Keratinous bioresources: their generation, microbial degradation, and value enhancement for biotechnological applications
Vijan Lal Vikash, Numbi Ramudu Kamini, Ganesan Ponesakki, Suresh Kumar Anandasadagopan
World Journal of Microbiology and Biotechnology.2025;[Epub] CrossRef

Assessment of Cre-lox and CRISPR-Cas9 as tools for recycling of multiple-integrated selection markers in Saccharomyces cerevisiae: Hye Yun Moon† , Gyu Hun Sim† , Hyeon Jin Kim , Keunpil Kim , Hyun Ah Kang; J. Microbiol. 2022;60(1):18-30. Published online December 29, 2021; DOI: https://doi.org/10.1007/s12275-022-1580-7

69 View
0 Download
7 Web of Science
7 Crossref

Abstract

We evaluated the Cre-lox and CRISPR-Cas9 systems as markerrecycling tools in Saccharomyces cerevisiae recombinants containing multiple-integrated expression cassettes. As an initial trial, we constructed rDNA-nontranscribed spacer- or Ty4- based multiple integration vectors containing the URA3 marker flanked by the loxP sequence. Integrants harboring multiple copies of tHMG1 and NNV-CP expression cassettes were obtained and subsequently transformed with the Cre plasmid. However, the simultaneous pop-out of the expression cassettes along with the URA3 marker hampered the use of Cre-lox as a marker-recycling tool in multiple integrants. As an alternative, we constructed a set of CRISPR-Cas9-gRNA vectors containing gRNA targeted to auxotrophic marker genes. Transformation of multiple integrants of tHMG1 and NNV-CP cassettes by the Cas9-gRNA vector in the presence of the URA3 (stop) donor DNA fragments generated the Ura- transformants retaining multiple copies of the expression cassettes. CRISPR-Cas9-based inactivation led to the recycling of the other markers, HIS3, LEU2, and TRP1, without loss of expression cassettes in the recombinants containing multiple copies of tHMG1, NNV-CP, and SfBGL1 cassettes, respectively. Reuse of the same selection marker in marker-inactivated S. cerevisiae was validated by multiple integrations of the TrEGL2 cassette into the S. cerevisiae strain expressing SfBGL1. These results demonstrate that introducing stop codons into selection marker genes using the CRISPR-Cas9 system with donor DNA fragments is an efficient strategy for markerrecycling in multiple integrants. In particular, the continual reuse of auxotrophic markers would facilitate the construction of a yeast cell factory containing multiple copies of expression cassettes without antibiotic resistance genes.

Citations

Citations to this article as recorded by

Multiple metabolic engineering of Saccharomyces cerevisiae for the production of lycopene
Jiaheng Liu, Minxia Song, Xianhao Xu, Yaokang Wu, Yanfeng Liu, Guocheng Du, Jianghua Li, Long Liu, Xueqin Lv
Food Bioengineering.2024;[Epub] CrossRef
Biochemical and Biorefinery Platform for Second-Generation Bioethanol: Fermentative Strategies and Microorganisms
Karla D. González-Gloria, Elia Tomás-Pejó, Lorena Amaya-Delgado, Rosa M. Rodríguez-Jasso, Araceli Loredo-Treviño, Anusuiya Singh, Meenu Hans, Carlos Martín, Sachin Kumar, Héctor A. Ruiz
Fermentation.2024; 10(7): 361. CrossRef
CRISPR/Cas9-based toolkit for rapid marker recycling and combinatorial libraries in Komagataella phaffii
Wei Zhou, Yuanyi Li, Guosong Liu, Weichuang Qin, Dongzhi Wei, Fengqing Wang, Bei Gao
Applied Microbiology and Biotechnology.2024;[Epub] CrossRef
Establishment, optimization, and application of genetic technology in Aspergillus spp.
Jing Gao, Huiqing Liu, Zhenzhen Zhang, Zhihong Liang
Frontiers in Microbiology.2023;[Epub] CrossRef
CRISPR-Cas Technology for Bioengineering Conventional and Non-Conventional Yeasts: Progress and New Challenges
Yuanyuan Xia, Yujie Li, Wei Shen, Haiquan Yang, Xianzhong Chen
International Journal of Molecular Sciences.2023; 24(20): 15310. CrossRef
Genomic and functional features of yeast species in Korean traditional fermented alcoholic beverage and soybean products
Da Min Jeong, Hyeon Jin Kim, Min-Seung Jeon, Su Jin Yoo, Hye Yun Moon, Eun-joo Jeon, Che Ok Jeon, Seong-il Eyun, Hyun Ah Kang
FEMS Yeast Research.2023;[Epub] CrossRef
Multiplex genome editing to construct cellulase engineered Saccharomyces cerevisiae for ethanol production from cellulosic biomass
Yatika Dixit, Preeti Yadav, Arun Kumar Sharma, Poornima Pandey, Arindam Kuila
Renewable and Sustainable Energy Reviews.2023; 187: 113772. CrossRef

Diversity and composition of microbiota during fermentation of traditional Nuodeng ham: Xiao-mei Zhang , Xi-jun Dang , Yuan-bing Wang , Tao Sun , Yao Wang , Hong Yu , Wu-song Yang; J. Microbiol. 2021;59(1):20-28. Published online December 23, 2020; DOI: https://doi.org/10.1007/s12275-021-0219-4

59 View
0 Download
14 Web of Science
12 Crossref

Abstract

The microbial community is one of the most important factors in shaping the characteristics of fermented food. Nuodeng ham, traditionally produced and subjected to 1–4 years of fermentation, is a dry fermented food product with cultural and economic significance to locals in southwestern China. In this study, we aimed to characterize the microbiota and physicochemical profiles of Nuodeng ham across different stages of fermentation. Ham samples from each of the four years were analyzed by sequencing bacterial 16S rRNA gene and fungal internal transcribed spacer sequence, in order to characterize the diversity and composition of their microflora. A total of 2,679,483 bacterial and 2,983,234 fungal sequences of high quality were obtained and assigned to 514 and 57 genera, respectively. Among these microbes, Staphylococcus and Candida were the most abundant genera observed in the ham samples, though samples from different years showed differences in their microbial abundance. Results of physicochemical properties (pH, water, amino acid, NaCl, nitrate and nitrite contents, and the composition of volatile compounds) revealed differences among the ham samples in the composition of volatile compounds, especially in the third year samples, in which no nitrite was detected. These results suggest that the structure and diversity of microbial communities significantly differed across different stages of fermentation. Moreover, the third year hams exhibits a unique and balanced microbial community, which might contribute to the special flavor in the green and safe food products. Thus, our study lends insights into the production of high quality Nuodeng ham.

Citations

Citations to this article as recorded by

Metabolite and microbial community composition of normal and sensory defect Nuodeng hams characterized based on metabolomics and high-throughput sequencing
Nannan Zhou, Yaying Zhao, Guiying Wang, Guanghui Chen, Zhijie Zheng, Ruwei Ren, Guozhou Liao
Food Chemistry.2025; 463: 141358. CrossRef
Insight into the Relationship between the Causes of Off-Odour and Microorganism Communities in Xuanwei Ham
Haoyi Wang, Xiaoyu Yin, Lu Zhang, Xuejiao Wang, Jiliang Zhang, Rongxin Wen, Jianxin Cao
Foods.2024; 13(5): 776. CrossRef
Study on the Changes and Correlation of Microorganisms and Flavor in Different Processing Stages of Mianning Ham
Yue Huang, Zhengli Wang, Ling Gan, Jiamin Zhang, Wei Wang, Lili Ji, Lin Chen
Foods.2024; 13(16): 2587. CrossRef
Revealing the correlation between small molecule metabolites, volatile compounds and microbial communities during the ripening of Xuanwei ham
Cong Li, Zhijie Zheng, Guiying Wang, Guanghui Chen, Nannan Zhou, Ruwei Ren, Qiongfang Yang, Wenxi Fu, Bo Li, Guozhou Liao
LWT.2024; 211: 116955. CrossRef
Changes in Physicochemical Characteristics and Microbial Diversity of Traditional Fermented Vinasse Hairtail
Yue Zhang, Chuanhai Tu, Huimin Lin, Yuwei Hu, Junqi Jia, Shanshan Shui, Jiaxing Wang, Yi Hu, Bin Zhang
Fermentation.2023; 9(2): 173. CrossRef
Recent developments in off-odor formation mechanism and the potential regulation by starter cultures in dry-cured ham
Changyu Zhou, Qiang Xia, Lihui Du, Jun He, Yangying Sun, Yali Dang, Fang Geng, Daodong Pan, Jinxuan Cao, Guanghong Zhou
Critical Reviews in Food Science and Nutrition.2023; 63(27): 8781. CrossRef
Role of microbiota and its ecological succession on flavor formation in traditional dry-cured ham: a review
Ping Li, Zhijie Bao, Yang Wang, Xinlian Su, Hui Zhou, Baocai Xu
Critical Reviews in Food Science and Nutrition.2023; : 1. CrossRef
Illumina-Based Analysis Yields New Insights Into the Fungal Contamination Associated With the Processed Products of Crataegi Fructus
Jingsheng Yu, Mengyue Guo, Wenjun Jiang, Yujie Dao, Xiaohui Pang
Frontiers in Nutrition.2022;[Epub] CrossRef
Evaluation of protein degradation and flavor compounds during the processing of Xuan'en ham
Rui Li, Cuizhu Geng, Zhemin Xiong, Yingying Cui, E Liao, Lijuan Peng, Weiping Jin, Haibin Wang
Journal of Food Science.2022; 87(8): 3366. CrossRef
Characterization and correlation of dominant bacteria and volatile compounds in post-fermentation process of Ba-bao Douchi
Yan-Zeng Zhang, Xiang-Na Lin, Yan-Qing Ji, Hong-Jun He, Hong-Zhuan Yang, Xiao-Juan Tang, Yun-Guo Liu
Food Research International.2022; 160: 111688. CrossRef
Microbial community composition and soil metabolism in the coexisting Cordyceps militaris and Ophiocordyceps highlandensis
Xiaorong Xu, Xiaomei Zhang, Zhipu Huang, Yuxiao Xu, Dexiang Tang, Bing Zhang, Ketao Zhang, Chaojin Liu, Hong Yu
Journal of Basic Microbiology.2022; 62(10): 1254. CrossRef
Metagenomic Analysis of Suansun, a Traditional Chinese Unsalted Fermented Food
Yaping Hu, Xiaodong Chen, Jie Zhou, Wenxuan Jing, Qirong Guo
Processes.2021; 9(9): 1669. CrossRef

Stenotrophomonas maltophilia outer membrane protein A induces epithelial cell apoptosis via mitochondrial pathways: Xin Wang , Yan Li , Xueping Tang , Xueyi Shang , Zunquan Zhao , Yongqiang Jiang , Yan Li; J. Microbiol. 2020;58(10):868-877. Published online September 2, 2020; DOI: https://doi.org/10.1007/s12275-020-0235-9

48 View
0 Download
9 Web of Science
4 Crossref

Abstract

Stenotrophomonas maltophilia (S. maltophilia) is a common opportunistic pathogen in intensive care units and causes infections most often after surgeries in immune-compromised patients such as those undergoing chemotherapy. Outer membrane protein A (OmpA) is the most abundant of the outer membrane proteins in S. maltophilia. Previous studies on OmpA usually focus on its interaction with the host cells and its role in vaccine development. However, the impact of OmpA on the virulence of S. maltophilia to host cells and the effects on apoptosis remain unclear. In this study, we exposed purified recombinant S. maltophilia OmpA (rOmpA) to HEp-2 cells and investigated the effects of OmpA on epithelial cell apoptosis. Morphologic and flow cytometric analyses revealed that HEp-2 cells stimulated with rOmpA multiple apoptosis features, including nuclear roundness and pyknosis, chromatin aggregation, and phosphatidylserine eversion. We found that rOmpA regulated the protein levels of Bax and Bcl-xL in HEp-2 cells, leading to changes in mitochondria permeability and the release of cytochrome c and apoptosis-inducing factors into the cytoplasm. These subsequently activate the caspase-9/caspase-3 pathway that promote apoptosis. We also observed that rOmpA enhanced the generation of reactive oxygen species and increased intracellular Ca2+ levels in HEp-2 cells. Collectively, our data suggested that rOmpA induced epithelial cells apoptosis via mitochondrial pathways.

Citations

Citations to this article as recorded by

Functional analysis of OmpA and its contribution to pathogenesis of Edwardsiella tarda
Quan Zhou, Yushuai Hu, Yicheng You, Yingli Gao, Xingqiang Wang, Lei Qin
Microbial Pathogenesis.2024; 193: 106760. CrossRef
Application of mPEG-CS-cRGD/ Bmi-1RNAi -PTX nanoparticles in suppression of laryngeal cancer by targeting cancer stem cells
Xiaoyan Xu, Tianhao Zhou, Xudong Wei, Xuelian Jiang, Jiyan Cao
Drug Delivery.2023;[Epub] CrossRef
Control of host mitochondria by bacterial pathogens
Saverio Marchi, Gianluca Morroni, Paolo Pinton, Lorenzo Galluzzi
Trends in Microbiology.2022; 30(5): 452. CrossRef
Lysophosphatidic acid protects cervical cancer HeLa cells from apoptosis induced by doxorubicin hydrochloride
Xibo Wang, Haihua Wang, Xiaoxiao Mou, Yilin Xu, Wenbo Han, Aimin Huang, Yanwei Li, Hui Jiang, Xiaoyun Yang, Zhenbo Hu
Oncology Letters.2022;[Epub] CrossRef

Short-term effects of returning granulated straw on soil microbial community and organic carbon fractions in dryland farming: Wei Fan , Jinggui Wu; J. Microbiol. 2020;58(8):657-667. Published online June 25, 2020; DOI: https://doi.org/10.1007/s12275-020-9266-5

66 View
0 Download
29 Web of Science
27 Crossref

Abstract

We conducted a 2-year field experiment which was comprised of five treatments, namely no straw returning (CK), straw mulching (SM), straw plowed into the soil (SP), and straw returned in granulated form (SG). The aim of this study was to investigate the effects of different straw returning modes on soil bacterial and fungal community structure and their relationships to soil organic carbon (SOC) fractions at three different soil depths (0–20, 20–40, and 40–60 cm) in a dryland under maize cultivation in Northeast (NE) China. SM, SP, and SG treatments significantly increased SOC content. Compared with SM and SP treatments, SG treatment significantly increased the content of SOC and easily oxidizable carbon (EOC) in the topsoil (0–20 cm depth), and increased dissolved organic carbon (DOC) and SOC content of the light fraction (LFOC) in the 20–40 cm layer. Meanwhile, SG treatment exhibited the highest microbial biomass C (MBC) content in all of the three soil depths. SG treatment also enhanced bacterial richness as well as fungal richness and diversity in the upper 40 cm of soil. In addition, SG treatment increased the relative abundance of Proteobacteria in all depths, and had the highest relative abundance of Basidiomycota in the first 20 cm of soil. SP treatment showed the lowest soil organic carbon content in all fractions and soil microbial community composition. SM treatment exhibited similar results to SG treatment in SOC, DOC, and LFOC contents, and bacterial diversity in the topsoil and subsoil. As a whole, treatment SG improved soil quality and maize yield, hence we recommend returning granulated straw as the most effective practice for enhancing labile SOC fractions as well as maintaining soil diversity and microbial richness of arid farmlands in NE China.

Citations

Citations to this article as recorded by

Evolutionary Behaviors of Straw-Reinforced Slurry for Sustainable Management of Dredging Sediment: Rheological and Fertility Properties
Chengchun Qiu, Liwei Xu, Weijuan Geng, Guizhong Xu, Dan Zhang
Waste and Biomass Valorization.2025; 16(3): 1057. CrossRef
Synergizing production and ecology: innovations in sustainable dryland agriculture
Xining Zhao, Honghua He, Kadambot H. M. Siddique, Xiaodong Gao
Plant and Soil.2024; 499(1-2): 1. CrossRef
Analysis of Tobacco Straw Return to the Field to Improve the Chemical, Physical, and Biological Soil Properties and Rice Yield
Jie Huang, Xinyue Wang, Lili Yang, Yuanhuan Li, Bing Xia, Hailin Li, Xiaohua Deng
Agronomy.2024; 14(5): 1025. CrossRef
Characteristics of Bacterial Communities under Different Tree Species and Their Response to Soil Physicochemical Properties
Zhe Chen, Suyan Li, Xiangyang Sun, Libing He, Wenzhi Zhou, Guanyu Zhao, Jiantao Yu, Xueting Bai, Jinshuo Zhang
Forests.2024; 15(5): 740. CrossRef
Effects of Different Straw Cover Patterns on Water Use Efficiency and Crop Growth
Han Lin, Mingyong Li, Jiangtao Ji, Chao Zhang, Guangyuan Zhong, Hongjian Wu, Zhaohui Du, Qianwen Li, Lu Tan, Haopeng Han
Agronomy.2024; 14(12): 2885. CrossRef
Successive Years of Rice Straw Return Increased the Rice Yield and Soil Nutrients While Decreasing the Greenhouse Gas Intensity
Meikang Wu, Min Nuo, Zixian Jiang, Ruiyao Xu, Hongcheng Zhang, Xiao Lu, Liqun Yao, Man Dou, Xu Xing, Xin Meng, Dongchao Wang, Xiaoshuang Wei, Ping Tian, Guan Wang, Zhihai Wu, Meiying Yang
Plants.2024; 13(17): 2446. CrossRef
Straw addition increases enzyme activities and microbial carbon metabolism activities in bauxite residue
Hao Wu, Wei Sun, Feng Zhu, Yifan Jiang, Shiwei Huang, Johnvie Goloran, Shengguo Xue
Journal of Environmental Sciences.2024; 135: 332. CrossRef
Impact of Straw Incorporation on the Physicochemical Profile and Fungal Ecology of Saline–Alkaline Soil
Weiming Ma, Li Ma, Jintang Jiao, Abbas Muhammad Fahim, Junyan Wu, Xiaolei Tao, Yintao Lian, Rong Li, Yapeng Li, Gang Yang, Lijun Liu, Yuanyuan Pu, Wancang Sun, Wangtian Wang
Microorganisms.2024; 12(2): 277. CrossRef
Different Impacts of Long-Term Tillage and Manure on Yield and N Use Efficiency, Soil Fertility, and Fungal Community in Rainfed Wheat in Loess Plateau
Mengni Chen, Hailiang Yang, Qingshan Yang, Yongshan Li, Hui Wang, Juanling Wang, Qiaolan Fan, Na Yang, Ke Wang, Jiancheng Zhang, Jiawei Yuan, Peng Dong, Lu Wang
Plants.2024; 13(24): 3477. CrossRef
Combined Application of Chemical and Organic Fertilizers Promoted Soil Carbon Sequestration and Bacterial Community Diversity in Dryland Wheat Fields
Hongmei Song, Zixuan Chang, Xuan Hu, Yan Li, Chengjiao Duan, Lifan Yang, Haoying Wang, Tingliang Li
Land.2024; 13(8): 1296. CrossRef
Continuous Straw Returning Combined with Nitrogen Application Improve Soil Properties and Yield of Double Cropping Maize in Subtropical Regions
Zhenwei Li, Kashif Khan, Li Yang, Yanqiong Pan, Xunbo Zhou
Sustainability.2024; 16(12): 5265. CrossRef
Changes in soil organic carbon and microbial community in saline soil following different forms of straw incorporation
Ting Fan, Yulin Zhang, Kexin Hu, Shiqi Xu, Afeng Zhang, Shaoqi Xue, Jiale Han, Xudong Wang
European Journal of Soil Science.2024;[Epub] CrossRef
Optimizing straw in rheology and compression–permeability of cemented dredged slurry
Guizhong Xu, Fahong Wu, Weijuan Geng
Marine Georesources & Geotechnology.2024; : 1. CrossRef
Enhancing phosphorus transformation in typical reddish paddy soil from China: Insights on long-term straw return and pig manure application via microbial mechanisms
Jian Xiao, Jianglin Zhang, Peng Li, Youyun Tang, Yanhong Lu, Yulin Liao, Jun Nie
Science of The Total Environment.2024; 940: 173513. CrossRef
Effects of Maize Straw Return Modes on Soil Organic Carbon Content and Aggregate Stability in a Mollisol in Northeast China
Y. Yuan, H. Liu, Y. Liang, J. Yuan, C. Zhang, J. Zhang, H. Cai, L. Wang
Moscow University Soil Science Bulletin.2024; 79(5): 693. CrossRef
Deep Straw Burial Accelerates Straw Decomposition and Improves Soil Water Repellency
Bo-Yan Zhang, Sen Dou, Song Guan, Chuang Yang, Zhao Wang
Agronomy.2023; 13(7): 1927. CrossRef
Application of various high- density organic materials in soil promotes germination and increases nutrient content of wheat
Ting Fan, Yulin Zhang, Xudong Wang, Yonghua Zhao, Andong Shi, Xia Zhang
Environmental Technology & Innovation.2023; 32: 103298. CrossRef
Returned straw reduces nitrogen runoff loss by influencing nitrification process through modulating soil C:N of different paddy systems
Shaopeng Wang, Limei Zhai, Shufang Guo, Fulin Zhang, Lingling Hua, Hongbin Liu
Agriculture, Ecosystems & Environment.2023; 354: 108438. CrossRef
Effects of rumen microorganisms on straw returning to soil at different depths
Kailun Song, Chunhuo Zhou, Hengpei Li, Zicheng Zhou, Guorong Ni, Xin Yin
European Journal of Soil Biology.2023; 114: 103454. CrossRef
Stover return and nitrogen application affect soil organic carbon and nitrogen in a double‐season maize field
Y. X. Liu, Y. Q. Pan, L. Yang, S. Ahmad, X. B. Zhou, M. Zhou
Plant Biology.2022; 24(2): 387. CrossRef
Native soil organic-carbon contents shape distinct bacterial communities associated with priming effect
Xu Chen, Mengyang You, Xiaozeng Han, Xinchun Lu, Wenxiu Zou, Jun Yan
Pedobiologia.2022; 95: 150842. CrossRef
Straw Return and Nitrogen Fertilization to Maize Regulate Soil Properties, Microbial Community, and Enzyme Activities Under a Dual Cropping System
Li Yang, Ihsan Muhammad, Yu Xin Chi, Dan Wang, Xun Bo Zhou
Frontiers in Microbiology.2022;[Epub] CrossRef
Effect of straw application time on soil properties and microbial community in the Northeast China Plain
Liwei Wang, Cheng Wang, Fangyuan Feng, Zhengniu Wu, Hongkui Yan
Journal of Soils and Sediments.2021; 21(9): 3137. CrossRef
Labile organic carbon fractions drive soil microbial communities after long-term fertilization
Zhiming Zhang, Jun Yan, Xiaozeng Han, Wenxiu Zou, Xu Chen, Xinchun Lu, Yutian Feng
Global Ecology and Conservation.2021; 32: e01867. CrossRef
Soil bacterial community as impacted by addition of rice straw and biochar
Zhiqiang Tang, Liying Zhang, Na He, Diankai Gong, Hong Gao, Zuobin Ma, Liang Fu, Mingzhu Zhao, Hui Wang, Changhua Wang, Wenjing Zheng, Wenzhong Zhang
Scientific Reports.2021;[Epub] CrossRef
Straw incorporation plus biochar addition improved the soil quality index focused on enhancing crop yield and alleviating global warming potential
Na Li, Shuyue Wen, Shikun Wei, Hongyang Li, Yongzhong Feng, Guangxin Ren, Gaihe Yang, Xinhui Han, Xiaojiao Wang, Chengjie Ren
Environmental Technology & Innovation.2021; 21: 101316. CrossRef
Dynamics of soil bacteria and fungi communities of dry land for 8 years with soil conservation management
Yue Yang, Yan'an Tong, Lian-you Liang, Hong-chang Li, Wen-she Han
Journal of Environmental Management.2021; 299: 113544. CrossRef

Review

MINIREVIEW] Shiga Toxins Expressed by Human Pathogenic Bacteria Induce Immune Responses in Host Cells: Moo-Seung Lee , Myung Hee Kim , Vernon L. Tesh; J. Microbiol. 2013;51(6):724-730. Published online December 19, 2013; DOI: https://doi.org/10.1007/s12275-013-3429-6

54 View
0 Download
21 Crossref

Abstract

Shiga toxins are a family of genetically and structurally related toxins that are the primary virulence factors produced by the bacterial pathogens Shigella dysenteriae serotype 1 and certain Escherichia coli strains. The toxins are multifunctional proteins inducing protein biosynthesis inhibition, ribotoxic and ER stress responses, apoptosis, autophagy, and inflammatory cytokine and chemokine production. The regulated induction of inflammatory responses is key to minimizing damage upon injury or pathogen-mediated infections, requiring the concerted activation of multiple signaling pathways to control cytokine/chemokine expression. Activation of host cell signaling cascades is essential for Shiga toxinmediated proinflammatory responses and the contribution of the toxins to virulence. Many studies have been reported defining the inflammatory response to Shiga toxins in vivo and in vitro, including production and secretion of tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), macrophage inflammatory protein-1α/β (MIP-1α/β), macrophage chemoattractant monocyte chemoattractant protein 1 (MCP-1), interleukin 8 (IL-8), interleukin 6 (IL-6), and Groβ. These cytokines and chemokines may contribute to damage in the colon and development of life threatening conditions such as acute renal failure (hemolytic uremic syndrome) and neurological abnormalities. In this review, we summarize recent findings in Shiga toxin-mediated inflammatory responses by different types of cells in vitro and in animal models. Signaling pathways involved in the inflammatory responses are briefly reviewed.

Citations

Citations to this article as recorded by

Involvement of aquaporins in Shiga toxin-induced swelling and water transport dysfunction in human renal microvascular endothelial cells
Fernando D. Gomez, Julieta Reppetti, Romina S. Alvarez, Daniel C. Girón Reyes, Flavia Sacerdoti, Alejandro Balestracci, Alicia E. Damiano, Nora A. Martínez, Gisela Di Giusto, María M. Amaral
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research.2025; 1872(1): 119866. CrossRef
Distribution characteristics of antibiotic resistance in direct-eating food and analysis of Citrobacter freundii genome and pathogenicity
Qing Wang, Xuening Wang, Shaojing Sun, Litao Wang, Yan Sun, Xinyan Guo, Na Wang, Bin Chen
World Journal of Engineering.2024; 21(6): 1095. CrossRef
Significance of Pulmonary Endothelial Injury and the Role of Cyclooxygenase-2 and Prostanoid Signaling
Rosa Nickl, Sandra Hauser, Jens Pietzsch, Torsten Richter
Bioengineering.2023; 10(1): 117. CrossRef
The predominance of Shiga toxin-producing E. coli in the Southeast Coast of India
Kannan Kamala, Shanmugam Rajeshkumar, Pitchiah Sivaperumal
Marine Pollution Bulletin.2022; 174: 113188. CrossRef
Gene expression profile and injury sites in mice treated with Shiga toxin 2 and lipopolysaccharide as a Shiga toxin-associated hemolytic uremic syndrome model
Yohei Kume, Hayato Go, Ryo Maeda, Kazuhide Suyama, Tsutomu Mori, Yukihiko Kawasaki, Koichi Hashimoto, Mitsuaki Hosoya
Physiological Genomics.2022; 54(5): 153. CrossRef
SARS-CoV-2 and Emerging Foodborne Pathogens: Intriguing Commonalities and Obvious Differences
Ahmed G. Abdelhamid, Julia N. Faraone, John P. Evans, Shan-Lu Liu, Ahmed E. Yousef
Pathogens.2022; 11(8): 837. CrossRef
Combined Action of Shiga Toxin Type 2 and Subtilase Cytotoxin in the Pathogenesis of Hemolytic Uremic Syndrome
Romina S. Álvarez, Fernando D. Gómez, Elsa Zotta, Adrienne W. Paton, James C. Paton, Cristina Ibarra, Flavia Sacerdoti, María M. Amaral
Toxins.2021; 13(8): 536. CrossRef
Shiga toxin remodels the intestinal epithelial transcriptional response to Enterohemorrhagic Escherichia coli
Alyson R. Warr, Carole J. Kuehl, Matthew K. Waldor, Igor Eric Brodsky
PLOS Pathogens.2021; 17(2): e1009290. CrossRef
Comparative Transcriptomics of Shiga Toxin-Producing and Commensal Escherichia coli and Cytokine Responses in Colonic Epithelial Cell Culture Infections
Lisa M. Harrison, David W. Lacher, Mark K. Mammel, Susan R. Leonard
Frontiers in Cellular and Infection Microbiology.2020;[Epub] CrossRef
Lactobacillus acidophilus attenuates toxin production by Vibrio cholerae and shigella dysenteriae following intestinal epithelial cells infection
Shabnam Zeighamy Alamdary, Bita Bakhshi
Microbial Pathogenesis.2020; 149: 104543. CrossRef
Roles of Shiga Toxins in Immunopathology
Moo-Seung Lee, Vernon L. Tesh
Toxins.2019; 11(4): 212. CrossRef
Nucleolar fibrillarin is an evolutionarily conserved regulator of bacterial pathogen resistance
Varnesh Tiku, Chun Kew, Parul Mehrotra, Raja Ganesan, Nirmal Robinson, Adam Antebi
Nature Communications.2018;[Epub] CrossRef
Simulation Study of cDNA Dataset to Investigate Possible Association of Differentially Expressed Genes of Human THP1-Monocytic Cells in Cancer Progression Affected by Bacterial Shiga Toxins
Syed A. Muhammad, Jinlei Guo, Thanh M. Nguyen, Xiaogang Wu, Baogang Bai, X. Frank Yang, Jake Y. Chen
Frontiers in Microbiology.2018;[Epub] CrossRef
Anti-inflammatory agents reduce microglial response, demyelinating process and neuronal toxin uptake in a model of encephalopathy produced by Shiga Toxin 2
Alipio Pinto, Clara Berdasco, David Arenas-Mosquera, Adriana Cangelosi, Patricia A. Geoghegan, Myriam C. Nuñez, Jorge Goldstein
International Journal of Medical Microbiology.2018; 308(8): 1036. CrossRef
Shiga Toxins as Multi-Functional Proteins: Induction of Host Cellular Stress Responses, Role in Pathogenesis and Therapeutic Applications
Moo-Seung Lee, Sunwoo Koo, Dae Jeong, Vernon Tesh
Toxins.2016; 8(3): 77. CrossRef
Shiga Toxins Activate the NLRP3 Inflammasome Pathway To Promote Both Production of the Proinflammatory Cytokine Interleukin-1β and Apoptotic Cell Death
Moo-Seung Lee, Haenaem Kwon, Eun-Young Lee, Dong-Jae Kim, Jong-Hwan Park, Vernon L. Tesh, Tae-Kwang Oh, Myung Hee Kim, B. A. McCormick
Infection and Immunity.2016; 84(1): 172. CrossRef
Angiotensin-(1–7) protects from brain damage induced by shiga toxin 2-producing enterohemorrhagicEscherichia coli
Jorge Goldstein, Tomás R. Carden, María J. Perez, Carlos A. Taira, Christian Höcht, Mariela M. Gironacci
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology.2016; 311(6): R1173. CrossRef
Bacteria, the endoplasmic reticulum and the unfolded protein response: friends or foes?
Jean Celli, Renée M. Tsolis
Nature Reviews Microbiology.2015; 13(2): 71. CrossRef
The roles of the virulence factor IpaB in Shigella spp. in the escape from immune cells and invasion of epithelial cells
Shih-Chun Yang, Chi-Feng Hung, Ibrahim A. Aljuffali, Jia-You Fang
Microbiological Research.2015; 181: 43. CrossRef
Update on molecular epidemiology of Shigella infection
Ila F.N. Lima, Alexandre Havt, Aldo A.M. Lima
Current Opinion in Gastroenterology.2015; 31(1): 30. CrossRef
Shiga Toxin (Stx) Classification, Structure, and Function
Angela R. Melton-Celsa, Vanessa Sperandio, Carolyn J. Hovde
Microbiology Spectrum.2014;[Epub] CrossRef

Research Support, Non-U.S. Gov'ts

DBA/2 Mouse as an Animal Model for Anti-influenza Drug Efficacy Evaluation: Jin Il Kim , Sehee Park , Sangmoo Lee , Ilseob Lee , Jun Heo , Min-Woong Hwang , Joon-Yong Bae , Donghwan Kim , Seok-Il Jang , Mee Sook Park , Man-Seong Park; J. Microbiol. 2013;51(6):866-871. Published online December 19, 2013; DOI: https://doi.org/10.1007/s12275-013-3428-7

41 View
0 Download
14 Crossref

Abstract

Influenza viruses are seasonally recurring human pathogens. Vaccines and antiviral drugs are available for influenza. However, the viruses, which often change themselves via antigenic drift and shift, demand constant efforts to update vaccine antigens every year and develop new agents with broad-spectrum antiviral efficacy. An animal model is critical for such efforts. While most human influenza viruses are unable to kill BALB/c mice, some strains have been shown to kill DBA/2 mice without prior adaptation. Therefore, in this study, we explored the feasibility of employing DBA/2 mice as a model in the development of anti-influenza drugs. Unlike the BALB/c strain, DBA/2 mice were highly susceptible and could be killed with a relatively low titer (50% DBA/2 lethal dose = 102.83 plaque-forming units) of the A/ Korea/01/2009 virus (2009 pandemic H1N1 virus). When treated with a neuraminidase inhibitor, oseltamivir phosphate, infected DBA/2 mice survived until 14 days postinfection. The reduced morbidity of the infected DBA/2 mice was also consistent with the oseltamivir treatment. Taking these data into consideration, we propose that the DBA/2 mouse is an excellent animal model to evaluate antiviral efficacy against influenza infection and can be further utilized for combination therapies or bioactivity models of existing and newly developed anti-influenza drugs.

Citations

Citations to this article as recorded by

Swine influenza A virus isolates containing the pandemic H1N1 origin matrix gene elicit greater disease in the murine model
Shelly J. Curran, Emily F. Griffin, Lucas M. Ferreri, Constantinos S. Kyriakis, Elizabeth W. Howerth, Daniel R. Perez, S. Mark Tompkins, Robert Paul de Vries
Microbiology Spectrum.2024;[Epub] CrossRef
Kinetic of the Antibody Response Following AddaVax-Adjuvanted Immunization with Recombinant Influenza Antigens
Ted. M. Ross, Naveen Gokanapudi, Pan Ge, Hua Shi, Robert A. Richardson, Spencer R. Pierce, Pedro Sanchez, Subhan Ullah, Eliana De Luca, Giuseppe A. Sautto
Vaccines.2022; 10(8): 1315. CrossRef
Peptidylarginine Deiminase 2 in Murine Antiviral and Autoimmune Antibody Responses
Aisha M. Mergaert, Michael F. Denny, Brock Kingstad-Bakke, Mandar Bawadekar, S. Janna Bashar, Thomas F. Warner, Marulasiddappa Suresh, Miriam A. Shelef, Baohui Xu
Journal of Immunology Research.2022; 2022: 1. CrossRef
The morphine/heroin vaccine decreased the heroin-induced antinociceptive and reinforcing effects in three inbred strains mouse
Susana Barbosa-Méndez, Maura Matus-Ortega, Ricardo Hernández-Miramontes, Alberto Salazar-Juárez
International Immunopharmacology.2021; 98: 107887. CrossRef
Animal models for the risk assessment of viral pandemic potential
Mee Sook Park, Jin Il Kim, Joon-Yong Bae, Man-Seong Park
Laboratory Animal Research.2020;[Epub] CrossRef
In Vivo Assessment of Antibody-Dependent Enhancement of Influenza B Infection
Gautham K Rao, Rodney A Prell, Steven T Laing, Stefanie C M Burleson, Allen Nguyen, Jacqueline M McBride, Crystal Zhang, Daniel Sheinson, Wendy G Halpern
Toxicological Sciences.2019; 169(2): 409. CrossRef
Targeting the proviral host kinase, FAK, limits influenza a virus pathogenesis and NFkB-regulated pro-inflammatory responses
Silke Bergmann, Husni Elbahesh
Virology.2019; 534: 54. CrossRef
Effects of heat-killed Lactobacillus plantarum against influenza viruses in mice
Sehee Park, Jin Il Kim, Joon-Yong Bae, Kirim Yoo, Hyunung Kim, In-Ho Kim, Man-Seong Park, Ilseob Lee
Journal of Microbiology.2018; 56(2): 145. CrossRef
Alternative Strategy for a Quadrivalent Live Attenuated Influenza Virus Vaccine
Zhimin Wan, Stivalis Cardenas Garcia, Jing Liu, Jefferson Santos, Silvia Carnaccini, Ginger Geiger, Lucas Ferreri, Daniela Rajao, Daniel R. Perez, Adolfo García-Sastre
Journal of Virology.2018;[Epub] CrossRef
PPARgamma Deficiency Counteracts Thymic Senescence
David Ernszt, Krisztina Banfai, Zoltan Kellermayer, Attila Pap, Janet M. Lord, Judit E. Pongracz, Krisztian Kvell
Frontiers in Immunology.2017;[Epub] CrossRef
Repeated Low-Dose Influenza Virus Infection Causes Severe Disease in Mice: a Model for Vaccine Evaluation
Yufeng Song, Xiang Wang, Hongbo Zhang, Xinying Tang, Min Li, Jufang Yao, Xia Jin, Hildegund C. J. Ertl, Dongming Zhou, D. S. Lyles
Journal of Virology.2015; 89(15): 7841. CrossRef
A broadly neutralizing human monoclonal antibody is effective against H7N9
Kannan Tharakaraman, Vidya Subramanian, Karthik Viswanathan, Susan Sloan, Hui-Ling Yen, Dale L. Barnard, Y. H. Connie Leung, Kristy J. Szretter, Tyree J. Koch, James C. Delaney, Gregory J. Babcock, Gerald N. Wogan, Ram Sasisekharan, Zachary Shriver
Proceedings of the National Academy of Sciences.2015; 112(35): 10890. CrossRef
Anti-influenza effect of Cordyceps militaris through immunomodulation in a DBA/2 mouse model
Hwan Hee Lee, Heejin Park, Gi-Ho Sung, Kanghyo Lee, Taeho Lee, Ilseob Lee, Man-seong Park, Yong Woo Jung, Yu Su Shin, Hyojeung Kang, Hyosun Cho
Journal of Microbiology.2014; 52(8): 696. CrossRef
Combination Effects of Peramivir and Favipiravir against Oseltamivir-Resistant 2009 Pandemic Influenza A(H1N1) Infection in Mice
Sehee Park, Jin Il Kim, Ilseob Lee, Sangmoo Lee, Min-Woong Hwang, Joon-Yong Bae, Jun Heo, Donghwan Kim, Seok-Il Jang, Hyejin Kim, Hee Jin Cheong, Jin-Won Song, Ki-Joon Song, Luck Ju Baek, Man-Seong Park, Balaji Manicassamy
PLoS ONE.2014; 9(7): e101325. CrossRef

Live/Dead State Is Not the Factor Influencing Adhesion Ability of Bifidobacterium animalis KLDS2.0603: Li-Qun Wang , Feng Zhao , Fei Liu , Xiang-Chen Meng; J. Microbiol. 2013;51(5):584-589. Published online September 14, 2013; DOI: https://doi.org/10.1007/s12275-013-2632-9

57 View
0 Download
13 Crossref

Abstract

Two essential requirements for probiotic bifidobacteria are that they be “live” and have “colonization” ability, following FAO/WHO guideline recommendations. The amount of research on the adhesion ability of bifidobacteria compares poorly with that of other probiotic bacteria, such as lactobacilli. The aim of the present study was to determine how gastrointestinal conditions affect the adhesion ability of bifidobacteria, and to investigate the relationship between the adhesion ability and the live/dead state of bifidobacteria. The adhesion ability of Bifidobacterium animalis KLDS2.0603 that had been subjected to the digestive enzymes, pepsin, trypsin, and proteinase K, was decreased significantly, but these treatments did not significantly change the strain’s survival rates, which were 98.78%, 97.60%, and 97.63% respectively. B. animalis KLDS2.0603 subjected to LiCl retained its adhesion ability but had a lower survival rate (59.28%) than the control group (P<0.01). B. animalis KLDS 2.0603 subjected to sodium metaperiodate exhibited higher adhesion ability than the control group (P<0.01), but the bacterial cells were killed totally. The results of transmission electron microscopy and laser scanning confocal microscopy showed that live/dead state of bifidobacteria was not one of the main factors that affected the adhesion ability of bifidobacteira, and that the substances affecting the adhesion ability of bifidobacteria were on the outer surface layer of the bifidobacterial cells. Our results also indicated that the substances related to the adhesion ability of bifidobacteria are proteinaceous. The above results will help us to understand the adhesion and colonization processes of bifidobacteria in the human gastrointestinal tract.

Citations

Citations to this article as recorded by

Highly Adhesive Lactiplantibacillus plantarum ZJ316: Structural Insights of Lipoteichoic Acid and Its Anti-Inflammatory Properties
Xin Zhao, Lina Ding, Qiuqiu Ye, Hangjia Zhang, Jiaqi Yin, Ping Li, Qing Gu, Jiarun Han
Journal of Agricultural and Food Chemistry.2025;[Epub] CrossRef
Alteration of the gut microbiome in patients with heart failure: A systematic review and meta-analysis
Jiayi Huang, Yongping Lin, Xiangwei Ding, Song Lin, Xin Li, Wei Yan, Minglong Chen
Microbial Pathogenesis.2024; 192: 106647. CrossRef
The Effects of Cellular Membrane Damage on the Long-Term Storage and Adhesion of Probiotic Bacteria in Caco-2 Cell Line
Jakub Kiepś, Wojciech Juzwa, Anna Olejnik, Anna Sip, Jolanta Tomaszewska-Gras, Radosław Dembczyński
Nutrients.2023; 15(15): 3484. CrossRef
Mechanism of Blood Cholesterol-Lowering Action of Parabiotics
Min Cheol Kim, Min-Goo Kim
Food Supplements and Biomaterials for Health.2023;[Epub] CrossRef
The Role of the PFNA Operon of Bifidobacteria in the Recognition of Host’s Immune Signals: Prospects for the Use of the FN3 Protein in the Treatment of COVID-19
Venera Z. Nezametdinova, Roman A. Yunes, Marina S. Dukhinova, Maria G. Alekseeva, Valery N. Danilenko
International Journal of Molecular Sciences.2021; 22(17): 9219. CrossRef
Comparison of microbial diversity in rumen and small intestine of Xinong Saanen dairy goats using 16S rRNA gene high-throughput sequencing
Cong Li, Yanan Geng, Ping Wang, Huaiping Shi, Jun Luo
Animal Production Science.2021; 62(14): 1379. CrossRef
Adhesion mechanisms of Bifidobacterium animalis subsp. lactis JCM 10602 to dietary fiber
Maria TANIGUCHI, Minori NAMBU, Yoshio KATAKURA, Shino YAMASAKI-YASHIKI
Bioscience of Microbiota, Food and Health.2021; 40(1): 59. CrossRef
Potential Immunomodulatory Activity of a Selected Strain Bifidobacterium bifidum H3-R2 as Evidenced in vitro and in Immunosuppressed Mice
Jiacui Shang, Feng Wan, Le Zhao, Xiangchen Meng, Bailiang Li
Frontiers in Microbiology.2020;[Epub] CrossRef
Antimicrobial and Immunomodulatory Effects of Bifidobacterium Strains: A Review
Hyun Jung Lim, Hea Soon Shin
Journal of Microbiology and Biotechnology.2020; 30(12): 1793. CrossRef
Using probiotics for type 2 diabetes mellitus intervention: Advances, questions, and potential
Zhongke Sun, Xuejiao Sun, Juan Li, Zhaoyang Li, Qingwei Hu, Lili Li, Xinqi Hao, Maoping Song, Chengwei Li
Critical Reviews in Food Science and Nutrition.2020; 60(4): 670. CrossRef
Effects of prebiotics on immunologic indicators and intestinal microbiota structure in perioperative colorectal cancer patients
Xiaoliang Xie, Yaqin He, Hai Li, Dong Yu, Li Na, Ting Sun, Dong Zhang, Xinrong Shi, Yuhan Xia, Tao Jiang, Shikuo Rong, Shaoqi Yang, Xiaoqiang Ma, Guangxian Xu
Nutrition.2019; 61: 132. CrossRef
Probiotic properties of Enterococcus strains isolated from traditional naturally fermented cream in China
Lidong Guo, Tingting Li, Yaru Tang, Lijie Yang, Guicheng Huo
Microbial Biotechnology.2016; 9(6): 737. CrossRef
Identification of surface-associated proteins of Bifidobacterium animalis ssp. lactis KLDS 2.0603 by enzymatic shaving
Dequan Zhu, Yu Sun, Fei Liu, Aili Li, Limei Yang, Xiang-Chen Meng
Journal of Dairy Science.2016; 99(7): 5155. CrossRef

Histological Alterations and Immune Response Induced by Pet Toxin During Colonization with Enteroaggregative Escherichia coli (EAEC) in a Mouse Model: Teresita Sainz , Julia Perez , Ma. Cristina Fresan , Veronica Flores , Luis Jimenez , Ulises Hernandez , Ismael Herrera , Carlos Eslava; J. Microbiol. 2002;40(2):91-97.

43 View
0 Download

Abstract: Enteroaggregative E. coli (EAEC) is an important aethiological causal agent of diarrhea in people of developed and undeveloped countries. Different in vitro and in vivo models have been proposed to study the pathogenic and immune mechanisms of EAEC infection. The aim of this study was to analyze whether BALB/c mice could be used as an animal model to study EAEC pathogenesis. Six-week-old BALB/c mice were inoculated with EAEC strain 042 (O44:H18) nalidixic acid resistant, and re-inoculated ten days after. Mice feces were monitored for the presence of the EAEC strain over a period of 20 days. Bacteria were enumerated on MacConkey agar containing 100 ug of nalidixic acid per ml. Results showed that 35% of the animals were colonized for 3 days, 15% for 5 and 10% for more than 7 days. After re-inoculation only 16% of the animals remained colonized for more than 3 days. During the necropsy, the intestinal fluid of some of the infected animals presented mucus and blood. Six of these fluids showed the presence of IgA antibodies against Pet toxin and IgG antibodies raised against the toxin were also detected in the animal serum. Histopathologic evidence confirms the stimulation of mucus hypersecretion, an increased amount of goblet cells and the presence of bacterial aggregates in the apical surfaces of intestinal epithelial cells. Edema was present in the submucosa. These results suggest that BALB/c mice could be used as an animal model for the in vivo study of EAEC infection.

First
Prev
Page of 1
Next
Last

Search