Journal of Microbiology

Journal Articles

Identification and characterization of a novel light-induced promoter for recombinant protein production in Pleurotus ostreatus: Chaomin Yin , Xiuzhi Fan , Kun Ma , Zheya Chen , Defang Shi , Fen Yao , Hong Gao , Aimin Ma; J. Microbiol. 2020;58(1):39-45. Published online November 4, 2019; DOI: https://doi.org/10.1007/s12275-020-9230-4

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A lectin gene (plectin) with a high level of expression was previously identified by comparative transcriptome analysis of Pleurotus ostreatus. In this study, we cloned a 733-bp DNA fragment from the start codon of the plectin gene. Sequence analysis showed that the plectin promoter (Plp) region contained several eukaryotic transcription factor binding motifs, such as the TATA-box, four possible CAAT-box, light responsiveness motifs and MeJA-responsiveness motifs. To determine whether the Plp promoter was a light-regulated promoter, we constructed an expression vector with the fused egfp-hph fragment under the control of the Plp promoter and transformed P. ostreatus mycelia via Agrobacterium tumefaciens. PCR and Southern blot analyses confirmed the Plpegfp- hph fragment was integrated into the chromosomal DNA of transformants. qRT-PCR, egfp visualization, and intracellular egfp determination experiments showed the Plp promoter could be a light-induced promoter that may be suitable for P. ostreatus genetic engineering. This study lays the foundation for gene homologous expression in P. ostreatus.

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The GATA transcription factor BcWCL2 regulates citric acid secretion to maintain redox homeostasis and full virulence in Botrytis cinerea
Weiheng Ren, Chen Qian, Dandan Ren, Yunfei Cai, Zhaohui Deng, Ning Zhang, Congcong Wang, Yiwen Wang, Pinkuan Zhu, Ling Xu, Regine Kahmann
mBio.2024;[Epub] CrossRef

An efficient Agrobacterium-mediated transformation method for aflatoxin generation fungus Aspergillus flavus: Guomin Han , Qian Shao , Cuiping Li , Kai Zhao , Li Jiang , Jun Fan , Haiyang Jiang , Fang Tao; J. Microbiol. 2018;56(5):356-364. Published online May 2, 2018; DOI: https://doi.org/10.1007/s12275-018-7349-3

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Abstract

Aspergillus flavus often invade many important corps and produce harmful aflatoxins both in preharvest and during storage stages. The regulation mechanism of aflatoxin biosynthesis in this fungus has not been well explored mainly due to the lack of an efficient transformation method for constructing a genome-wide gene mutant library. This challenge was resolved in this study, where a reliable and efficient Agrobacterium tumefaciens-mediated transformation (ATMT) protocol for A. flavus NRRL 3357 was established. The results showed that removal of multinucleate conidia, to collect a homogenous sample of uninucleate conidia for use as the transformation material, is the key step in this procedure. A. tumefaciens strain AGL-1 harboring the ble gene for zeocin resistance under the control of the gpdA promoter from A. nidulans is suitable for genetic transformation of this fungus. We successfully generated A. flavus transformants with an efficiency of ~ 60 positive transformants per 106 conidia using our protocol. A small-scale insertional mutant library (~ 1,000 mutants) was constructed using this method and the resulting several mutants lacked both production of conidia and aflatoxin biosynthesis capacity. Southern blotting analysis demonstrated that the majority of the transformants contained a single T-DNA insert on the genome. To the best of our knowledge, this is the first report of genetic transformation of A. flavus via ATMT and our protocol provides an effective tool for construction of genome-wide gene mutant libraries for functional analysis of important genes in A. flavus.

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Agrobacterium tumefaciens-mediated transformation for the genetic modification of the biotechnologically relevant fungus Aspergillus vadensis through synthetic biology
Carolina Ropero-Pérez, Paloma Manzanares, Jose F. Marcos, Sandra Garrigues
Current Research in Biotechnology.2024; 7: 100178. CrossRef
Development of Green Fluorescent Protein-Tagged Strains of Fusarium acuminatum via PEG-Mediated Genetic Transformation
Fangyi Ju, Zhongqiang Qi, Jiajin Tan, Tingting Dai
Microorganisms.2024; 12(12): 2427. CrossRef
An efficient targeted gene deletion approach for Cochliobolus heterostrophus using Agrobacterium tumefaciens-mediated transformation
Jiaying Sun, Rui Yang, Yujia Liu, Zengran Zhou, Jiaqi Jia, Hongming Huang, Shuqin Xiao, Chunsheng Xue
Journal of Microbiological Methods.2024; 216: 106863. CrossRef
Establishment of an Agrobacterium tumefaciens-Mediated Transformation System for Hirsutella sinensis
Lijuan Wu, Xinkun Hu, Shen Yan, Zenglin Wu, Xuzhong Tang, Lei Xie, Yujie Qiu, Rui Li, Ji Chen, Mengliang Tian
Current Issues in Molecular Biology.2024; 46(9): 10618. CrossRef
Role of Flavohemoglobins in the Development and Aflatoxin Biosynthesis of Aspergillus flavus
Xiaoling Zhou, Dongyue Chen, Min Yu, Yuan Jiao, Fang Tao
Journal of Fungi.2024; 10(6): 437. CrossRef
HacA, a key transcription factor for the unfolded protein response, is required for fungal development, aflatoxin biosynthesis and pathogenicity of Aspergillus flavus
Min Yu, Xiaoling Zhou, Dongyue Chen, Yuan Jiao, Guomin Han, Fang Tao
International Journal of Food Microbiology.2024; 417: 110693. CrossRef
Synthetic Biology Tools for Engineering Aspergillus oryzae
Hui Yang, Chaonan Song, Chengwei Liu, Pengchao Wang
Journal of Fungi.2024; 10(1): 34. CrossRef
Construction of Cordycepin High-Production Strain and Optimization of Culture Conditions
Hui Zhang, Ping Chen, Lin Xu, De Xu, Wendi Hu, Yong Cheng, Shengli Yang
Current Microbiology.2023;[Epub] CrossRef
Agrobacterium tumefaciens-mediated transformation of Nigrospora sp. isolated from switchgrass leaves and antagonistic toward plant pathogens
Summi Dutta, Gabriella Houdinet, Gitanjali NandaKafle, Arjun Kafle, Christine V. Hawkes, Kevin Garcia
Journal of Microbiological Methods.2023; 215: 106849. CrossRef
Systematic Characterization of bZIP Transcription Factors Required for Development and Aflatoxin Generation by High-Throughput Gene Knockout in Aspergillus flavus
Qianqian Zhao, Hao Pei, Xiaoling Zhou, Kai Zhao, Min Yu, Guomin Han, Jun Fan, Fang Tao
Journal of Fungi.2022; 8(4): 356. CrossRef
Homologous Expression and Characterization of α-L-rhamnosidase from Aspergillus niger for the Transformation of Flavonoids
Hangyu Ye, Xiaojun Li, Luyuan Li, Yinjun Zhang, Jianyong Zheng
Applied Biochemistry and Biotechnology.2022; 194(8): 3453. CrossRef
Genetic Manipulation and Transformation Methods for Aspergillus spp.
Ye-Eun Son, Hee-Soo Park
Mycobiology.2021; 49(2): 95. CrossRef
Homologous overexpression of genes in Cordyceps militaris improves the production of polysaccharides
Yifeng Wang, Xi Yang, Ping Chen, Shengli Yang, Hui Zhang
Food Research International.2021; 147: 110452. CrossRef
A Novel Site-Specific Integration System for Genetic Modification of Aspergillus flavus
Fang Tao, Kai Zhao, Qianqian Zhao, Fangzhi Xiang, Guomin Han
G3 Genes|Genomes|Genetics.2020; 10(2): 605. CrossRef
Identification of antibiotics for use in selection of the chytrid fungi Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans
Kristyn A. Robinson, Mallory Dunn, Shane P. Hussey, Lillian K. Fritz-Laylin, Louise A. Rollins-Smith
PLOS ONE.2020; 15(10): e0240480. CrossRef
Aromatic Polyketides from a Symbiotic Strain Aspergillus fumigatus D and Characterization of Their Biosynthetic Gene D8.t287
Yi Hua, Rui Pan, Xuelian Bai, Bin Wei, Jianwei Chen, Hong Wang, Huawei Zhang
Marine Drugs.2020; 18(6): 324. CrossRef
An optimized Agrobacterium tumefaciens-mediated transformation system for random insertional mutagenesis in Fonsecaea monophora
Xing Xiao, Yu Li, JingLin Qin, Ya He, Wenying Cai, Zhiwen Chen, Liyan Xi, Junmin Zhang
Journal of Microbiological Methods.2020; 170: 105838. CrossRef
Genome-wide association study leads to novel genetic insights into resistance to Aspergillus flavus in maize kernels
Guomin Han, Cuiping Li, Fangzhi Xiang, Qianqian Zhao, Yang Zhao, Ronghao Cai, Beijiu Cheng, Xuewen Wang, Fang Tao
BMC Plant Biology.2020;[Epub] CrossRef
The Efficacy of Composite Essential Oils against Aflatoxigenic Fungus Aspergillus flavus in Maize
Fangzhi Xiang, Qianqian Zhao, Kai Zhao, Hao Pei, Fang Tao
Toxins.2020; 12(9): 562. CrossRef
Ethylene and Benzaldehyde Emitted from Postharvest Tomatoes Inhibit Botrytis cinerea via Binding to G-Protein Coupled Receptors and Transmitting with cAMP-Signal Pathway of the Fungus
Yongwen Lin, Hongchun Ruan, Komivi Senyo Akutse, Baochun Lai, Yizhang Lin, Youming Hou, Fenglin Zhong
Journal of Agricultural and Food Chemistry.2019; 67(49): 13706. CrossRef

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Characterization of Plant-Growth Promoting Diazotrophic Bacteria Isolated from Field Grown Chinese Cabbage under Different Fertilization Conditions: Woo-Jong Yim , Selvaraj Poonguzhali , Munusamy Madhaiyan , Pitchai Palaniappan , M. A. Siddikee , Tongmin Sa; J. Microbiol. 2009;47(2):147-155. Published online May 2, 2009; DOI: https://doi.org/10.1007/s12275-008-0201-4

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Abstract: Diazotrophic bacteria isolated from the rhizosphere of Chinese cabbage were assessed for other plant growth promoting characteristics viz., production of IAA, ethylene, ACC deaminase, phosphate solubilization, and gnotobiotic root elongation. Their effect on inoculation to Chinese cabbage was also observed under growth chamber conditions. A total of 19 strains that showed higher nitrogenase activity identified by 16S rRNA gene sequence analysis were found to be the members of the genera Pseudomonas and Agrobacterium belonging to α- and γ-Proteobacteria groups. These strains were also efficient in producing IAA and ACC deaminase though they produced low levels of ethylene and no phosphate solubilization. In addition, inoculation of selected diazotrophic bacterial strains significantly increased seedling length, dry weight, and total nitrogen when compared to uninoculated control. The colonization of crop plants by diazotrophic bacteria can be affected by many biotic and abiotic factors, and further studies are oriented towards investigating the factors that could influence the establishment of a selected bacterial community.

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