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- Purification, crystallization, and preliminary X-ray crystallographic analysis of the Group III chaperonin from Carboxydothermus hydrogenoformans
- Young Jun An , Sara E. Rowland , Frank T. Robb , Sun-Shin Cha
- J. Microbiol. 2016;54(6):440-444. Published online May 27, 2016
- DOI: https://doi.org/10.1007/s12275-016-6089-5
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Abstract
-
Chaperonins (CPNs) are megadalton sized ATP-dependent
nanomachines that facilitate protein folding through complex
cycles of complex allosteric articulation. They consist of
two back-to-back stacked multisubunit rings. CPNs are usually
classified into Group I and Group II. Here, we report the
crystallization of both the AMPPNP (an ATP analogue) and
ADP bound forms of a novel CPN, classified as belonging to
a third Group, recently discovered in the extreme thermophile
Carboxydothermus hydrogenoformans. Crystals of the
two forms were grown by the vapor batch crystallization
method
at 295 K. Crystals of the Ch-CPN/AMPPNP complex diffracted to 3.0 Å resolution and belonged to the space group P422, with unit-cell parameters a = b = 186.166, c = 160.742 Å. Assuming the presence of four molecules in the asymmetric unit, the solvent content was estimated to be about 60.02%. Crystals of the Ch-CPN/ADP complex diffracted to 4.0 Å resolution and belonged to the space group P4212, with unit-cell parameters a = b = 209.780, c = 169.813Å. Assuming the presence of four molecules in the asymmetric unit, the solvent content was estimated to be about 70.19%. -
Citations
Citations to this article as recorded by
- Bridging human chaperonopathies and microbial chaperonins
Everly Conway de Macario, Masafumi Yohda, Alberto J. L. Macario, Frank T. Robb
Communications Biology.2019;[Epub] CrossRef - Prokaryotic Chaperonins as Experimental Models for Elucidating Structure-Function Abnormalities of Human Pathogenic Mutant Counterparts
Everly Conway de Macario, Frank T. Robb, Alberto J. L. Macario
Frontiers in Molecular Biosciences.2017;[Epub] CrossRef - Structural and mechanistic characterization of an archaeal-like chaperonin from a thermophilic bacterium
Young Jun An, Sara E. Rowland, Jung-Hyun Na, Dario Spigolon, Seung Kon Hong, Yeo Joon Yoon, Jung-Hyun Lee, Frank T. Robb, Sun-Shin Cha
Nature Communications.2017;[Epub] CrossRef
- Bridging human chaperonopathies and microbial chaperonins
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