Journal Article
- D-Galactose as an autoinducer 2 inhibitor to control the biofilm formation of periodontopathogens
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Eun-Ju Ryu , Jaehyun Sim , Jun Sim , Julian Lee , Bong-Kyu Choi
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J. Microbiol. 2016;54(9):632-637. Published online August 31, 2016
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DOI: https://doi.org/10.1007/s12275-016-6345-8
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Abstract
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Autoinducer 2 (AI-2) is a quorum sensing molecule to which
bacteria respond to regulate various phenotypes, including
virulence and biofilm formation. AI-2 plays an important role
in the formation of a subgingival biofilm composed mostly of
Gram-negative anaerobes, by which periodontitis is initiated.
The aim of this study was to evaluate D-galactose as an inhibitor
of AI-2 activity and thus of the biofilm formation of
periodontopathogens. In a search for an AI-2 receptor of
Fusobacterium nucleatum, D-galactose binding protein (Gbp,
Gene ID FN1165) showed high sequence similarity with
the ribose binding protein (RbsB), a known AI-2 receptor of
Aggregatibacter actinomycetemcomitans. D-Galactose was
evaluated for its inhibitory effect on the AI-2 activity of Vibrio
harveyi BB152 and F. nucleatum, the major coaggregation
bridge organism, which connects early colonizing commensals
and late pathogenic colonizers in dental biofilms. The
inhibitory effect of D-galactose on the biofilm formation of
periodontopathogens was assessed by crystal violet staining
and confocal laser scanning microscopy in the absence or
presence of AI-2 and secreted molecules of F. nucleatum.
D-Galactose significantly inhibited the AI-2 activity of V.
harveyi and F. nucleatum. In addition, D-galactose markedly
inhibited the biofilm formation of F. nucleatum, Porphyromonas
gingivalis, and Tannerella forsythia induced by the
AI-2 of F. nucleatum without affecting bacterial growth. Our
results
demonstrate that the Gbp may function as an AI-2
receptor and that galactose may be used for prevention of the
biofilm formation of periodontopathogens by targeting AI-2
activity.
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Research Support, Non-U.S. Gov'ts
- Inhibition of quorum sensing, biofilm, and spoilage potential in Shewanella baltica by green tea polyphenols
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Junli Zhu , Xuzheng Huang , Fang Zhang , Lifang Feng , Jianrong Li
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J. Microbiol. 2015;53(12):829-836. Published online December 2, 2015
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DOI: https://doi.org/10.1007/s12275-015-5123-3
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Abstract
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We investigated the quorum sensing (QS) system of Shewanella
baltica and the anti-QS related activities of green tea
polyphenols (TP) against spoilage bacteria in refrigerated large
yellow croaker. Autoinducer-2 (AI-2) and the diketopiperazines
(DKPs) cyclo-(L-Pro-L-Leu) and cyclo-(L-Pro-L-Phe) were
detected in the culture extract of S. baltica XH2, however,
no N-acylhomoserine lactones (AHLs) activity was observed.
Green TP at sub-inhibitory concentrations interfered with
AI-2 and DKPs activities of S. baltica without inhibiting
cell growth and promoted degradation of AI-2. The green TP
treatment inhibited biofilm development, exopolysaccharide
production and swimming motility of S. baltica in a concentration-
dependent manner. In addition, green TP decreased
extracellular protease activities and trimethylamine production
in S. baltica. A transcriptional analysis showed that green
TP repressed the luxS and torA genes in S. baltica, which
agreed with the observed reductions in QS activity and the
spoilage phenotype. Epigallocatechin gallate (EGCG)-enriched
in green TP significantly inhibited AI-2 activity of S.baltica.
These findings strongly suggest that green TP could be developed
as a new QS inhibitor for seafood preservation to
enhance shelf life.
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- Functional Definition of LuxS, an Autoinducer-2 (AI-2) Synthase and Its Role in Full Virulence of Streptococcus suis Serotype 2
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Min Cao , Youjun Feng , Changjun Wang , Feng Zheng , Ming Li , Hui Liao , Yinghua Mao , Xiuzhen Pan , Jing Wang , Dan Hu , Fuquan Hu , Jiaqi Tang
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J. Microbiol. 2011;49(6):1000-1011. Published online December 28, 2011
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DOI: https://doi.org/10.1007/s12275-011-1523-1
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Abstract
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Quorum sensing is a widespread chemical communication in response to fluctuation of bacterial population density, and has been implicated into bacterial biofilm formation and regulation of expression of virulence factors. The luxS gene product, S-ribosylhomocysteinase, catalizes the last committed step in biosynthetic pathway of autoinducer 2 (AI-2), a signaling molecule for inter-species quorum sensing. We found a luxS homologue in 05ZYH33, an epidemic strain of Streptococcus suis serotype 2 (SS2) in China. A luxS null mutant (ΔluxS) of 05ZYH33 strain was obtained using an approach of homologous recombination. LuxS was determined to be required for AI-2 production in 05ZYH33 strain of S. suis 2. Inactivation of luxS gene led to a wide range of phenotypic changes including thinner capsular walls, increased tolerance to H2O2, reduced adherence capacity to epithelial cells, etc. In particular, loss of LuxS impaired dramatically its full virulence of SS2 in experimental model of piglets, and functional complementation restored it nearly to the level of parent strain. Genome-wide transcriptome analyses suggested that some known virulence factors such as CPS are down-regulated in the ΔluxS mutant, which might in part explain virulence attenuation by luxS deletion. Similarly, 29 of 71 genes with different expression level were proposed to be targets candidate regulated by LuxS/AI-2-dependent quorum sensing.