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- Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription
- Hyung-Ju Lim , Kwangsoo Kim , Minsang Shin , Jae-Ho Jeong , Phil Youl Ryu , Hyon E. Choy
- J. Microbiol. 2015;53(4):250-255. Published online April 8, 2015
- DOI: https://doi.org/10.1007/s12275-015-4681-8
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- 3 Crossref
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Abstract
-
σ38 in Escherichia coli is required for expression of a subset
of stationary phase genes. However, the promoter elements
for σ38-dependent genes are virtually indistinguishable from
that for σ70-dependent house-keeping genes. hdeABp is a
σ38-dependent promoter and LEE5p is a σ70-dependent
promoter, but both are repressed by H-NS, a bacterial histone-
like protein, which acts at promoter upstream sequence.
We swapped the promoter upstream sequences of the two
promoters and found that the σ dependency was switched.
This was further verified using lacUV5 core promoter. The
results
suggested that the determinant for σ38-dependent promoter lies in the promoter upstream sequence. -
Citations
Citations to this article as recorded by
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- Sequence-dependent model of genes with dual σ factor preference
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