Journal of Microbiology

Detection of L-xylosone and its physiological effects in saccharomyces cerevisiae: Seok, Yeong Jae , Yang, Kap Seok , Kang, Ju Gyeong , Kim, Seong Tae , Huh, Won Ki , Kang, Sa Ouk; J. Microbiol. 1996;34(2):192-197.

Abstract: L-Xylosone was detected as its quinoxaline derivative in the degradation solution of dehydro-L-ascorbic acid. The production rate of L-xylosone was much faster in aerated phosphate-cirate buffer (pH 5. 6) than in pure water. L-Xylosone and dehydro-L-ascorbic acid were identified in the crude extracts of Saccharomyces cerevisiae. The concentration of L-xylosone in the crude cell extracts was calculated to be about 0.2 nmol (mg protein)^-1. When L-xylosone was added to asynchronous culture of S. cerevisiae, it inhibited primarily the synthesis of protein and RNA. Examination of the effect of L- xylosone on synchronous culture of the yeast indicated that L-xylosone inhibited the initiation of DNA replication and that the cells were arrested at G₁stage of cell division cycle. These results suggested a possibility that L-xylosone can act as an inhibitor of cell growth.

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