Journal of Microbiology

Dae Kyun Chung

4 Articles

Water-based extracts of Zizania latifolia inhibit Staphylococcus aureus infection through the induction of human beta-defensin 2 expression in HaCaT cells: Bo Yeon Kang , Seung-Su Lee , Myun-Ho Bang , Hyoik Jeon , Hangeun Kim , Dae Kyun Chung; J. Microbiol. 2018;56(12):910-916. Published online November 27, 2018; DOI: https://doi.org/10.1007/s12275-018-8307-9

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Zizania latifolia is a perennial herb belonging to the family Gramineae that has been used as a health food in Asian countries. In this study, we investigated the antimicrobial effect of Z. latifolia, which increased human beta-defensin 2 (hBD2) expression in HaCaT cells. hBD2 expression was further increased in cells treated with Z. latifolia extracts and subsequently infected with Staphylococcus aureus. Inversely, S. aureus infection decreased after treatment. The induction of hBD2 in HaCaT cells was mediated by the Toll-like receptor 2 (TLR2) signaling pathway, including the activation of extracellular signal-regulated kinase (ERK) and activator protein 1 (AP-1). Further study using siRNA revealed that hBD2 played an important role in the inhibition of S. aureus infection in HaCaT cells. Our data suggest that Z. latifolia extracts can be used as an antimicrobial ingredient for skin treatment formulas.

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Recent advances in Zizania latifolia : A comprehensive review on phytochemical, health benefits and applications that maximize its value
Weijie Wu, Yanchao Han, Ben Niu, Baiqi Yang, Ruiling Liu, Xiangjun Fang, Huizhi Chen, Shangyue Xiao, Mohamed A. Farag, Shiqi Zheng, Jianbo Xiao, Hangjun Chen, Haiyan Gao
Critical Reviews in Food Science and Nutrition.2024; 64(21): 7535. CrossRef
Application of melatonin delays lignification in postharvest water bamboo shoots in association with energy metabolism
Baiqi Yang, Yanchao Han, Haiyan Gao, Ruiling Liu, Feng Xu, Ruihai Liu, Shangyue Xiao, Bin Li, Hangjun Chen
Postharvest Biology and Technology.2023; 196: 112149. CrossRef
Toll-Like Receptors Signaling Pathway of Quercetin Regulating Avian Beta-Defensin in the Ileum of Broilers
Linlin Ying, Hao Wu, Shuaishuai Zhou, Han Lu, Manyi Ding, Bo Wang, Shanshan Wang, Yanjun Mao, Fenglin Xiao, Yao Li
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef
Potential Synergistic Action of Bioactive Compounds from Plant Extracts against Skin Infecting Microorganisms
Przemysław Sitarek, Anna Merecz-Sadowska, Tomasz Kowalczyk, Joanna Wieczfinska, Radosław Zajdel, Tomasz Śliwiński
International Journal of Molecular Sciences.2020; 21(14): 5105. CrossRef
Identification and expression analysis of chitinase genes in Zizania latifolia in response to abiotic stress
Niannian Zhou, Yulan An, Zhicheng Gui, Shuangshuang Xu, Xiaomei He, Jie Gao, Donglin Zeng, Defang Gan, Wenjuan Xu
Scientia Horticulturae.2020; 261: 108952. CrossRef

Oral administration of Lactobacillus plantarum lysates attenuates the development of atopic dermatitis lesions in mouse models: Hangeun Kim , Hye Rim Kim , Na-Ra Kim , Bong Jun Jeong , Jong Suk Lee , Soojin Jang , Dae Kyun Chung; J. Microbiol. 2015;53(1):47-52. Published online December 4, 2014; DOI: https://doi.org/10.1007/s12275-015-4483-z

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Abstract PDF

Lactobacillus plantarum is a well-documented probiotic that has been used in clinical trials for the regulation of the immune system and treatment of gastrointestinal diseases. In this study, we evaluated the effects of L. plantarum cell lysates on the immune regulation through the in vitro and in vivo studies. L. plantarum lysates were prepared by sonication
method
, and we observed that the repetition of disruption step increased indicator components within the bacterial lysates. Indicator components might affect TNF-α production. L. plantarum lysates did not induce TNF-α production, while LPS-induced TNF-α production was dramatically inhibited in a sonication-dependent manner in THP-1 cells. Oral administration of L. plantarum lysates effectively attenuated the horny layer formation and decreased epidermal thickening in NC/Nga mice skin. The damage to barrier function after the 8 weeks oral administration was reduced by L. plantarum lysates as compared to that in the atopic dermatitis (AD) mice. Further study revealed that L. plantarum lysates polarized Th1 response via induction of IL-12 and IFN-γ production and inhibition of IL-4 and IgE production in NC/Nga mice. Together, our results suggest that L. plantarum lysates are remarkable material for host homeostasis and it could be used for the treatment of inflammatory diseases.

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Lactoplantibacillus plantarum KG Lysates Inhibit the Internalization of Staphylococcus aureus by Human Keratinocytes through the Induction of Human Beta-Defensin 3
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Overexpression of Fish DRG2 Induces Cell Rounding: Jeong Jae Park , Seung Ju Cha , Myoung Seok Ko , Wha Ja Cho , Won Joon Yoon , Chang Hoon Moon , Jeong Wan Do , Sung Bum Kim , Hebok Song , Dae Kyun Chung , In Seob Han , KyuBum Kwack , Jeong Woo Park; J. Microbiol. 2002;40(4):295-300.

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Abstract PDF: Previously, we reported induced expression of developmentally regulated GTP-binding protein 2 (DRG2) in fish cells at the late stage of rhabdovirus infection. To investigate the biological role of fish DRG2 (fDRG2), we transfected CHSE-214 cells with an expression vector containing complete fDRG2 fused to the N-terminal end of an enhanced green fluorescent protein (EGFP). Low level expression of fDRG2-EGFP did not induce morphological change or cell death. However, a high level expression of fDRG2-EGFP induced cell rounding and caused depletion of the cell population in FACS analysis. Several truncated fragments were fused to EGFP. FACS analysis was conducted to determine the presence of cells expressing high levels of the resulting chimera. While cells expressing a high level of N-terminus were detected, those expressing high levels of the C-terminal fragment 243-290 containing the G4 motif were absent in FACS analysis. Based on these observations, we propose that overexpression of fDRG2 may induce cell rounding, a representative cytopathic effect of virus-infected cells in the late stage of infection and the C-terminus of the fDRG2 is essential for this function.

Expression of the Galactokinase Gene (galK) from Lactococcus lactis ssp. lactis ATCC7962 in Escherichia coli: Jae Yeon Choi , Jong-Hoon Lee , Jung Min Lee , Jeong Hwan Kim , Hae Choon Chang , Dae Kyun Chung , Somi Kim Cho , Hyong Joo Lee; J. Microbiol. 2002;40(2):156-160.

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Abstract PDF: The whole gal/lac operon genes of Lactococcus lactis ssp. lactis 7962 were reported as follows: galA-galM-galK-galT-lacA-lacZ-galE. The galK gene encoding a galactokinase involved in one of the Leloir pathways for galactose metabolism was found to be 1,197 bp in length and encodes a protein of 43,822 Da calculated molecular mass. The deduced amino acid sequence showed over 50% homology with GalK proteins from several other lactic acid bacteria. The galK gene was expressed in E. coli and the product was identified as a 43 kDa protein which corresponds to the estimated size from the DNA sequence. The galactokinase activity of recombinant E. coli was about 8 times greater against that of the host strain and more than 3 times higher than the induced L. lactis 7962.

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