- Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting
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Daisy Pérez-Brito , Anuar Magaña-Alvarez , Patricia Lappe-Oliveras , Alberto Cortes-Velazquez , Claudia Torres-Calzada , Teófilo Herrera-Suarez , Alfonso Larqué-Saavedra , Raul Tapia-Tussell
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J. Microbiol. 2015;53(1):14-20. Published online January 4, 2015
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DOI: https://doi.org/10.1007/s12275-015-4373-4
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This study characterized Clavispora lusitaniae strains isolated
from different stages of the processing and early fermentation
of a henequen (Agave fourcroydes) spirit produced in
Yucatan, Mexico using a molecular technique. Sixteen strains
identified based on morphological features, obtained from
different substrates, were typed molecularly. Nine different
versions of the divergent D1/D2 domain of the large-subunit
ribosomal DNA sequence were identified among the
C. lusitaniae strains. The greatest degree of polymorphism
was found in the 90-bp structural motif of the D2 domain.
The MSP-PCR technique was able to differentiate 100% of
the isolates. This study provides significant insight into the
genetic diversity of the mycobiota present during the henequen
fermentation process, especially that of C. lusitaniae,
for which only a few studies in plants have been published.
The applied MSP-PCR markers were very efficient in revealing
polymorphisms between isolates of this species.
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Citations
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