- Immunological charaterization of monoclonal antibodies used in rapid influenza diagnostic test for detection of the 2009 pandemic influenza A(H1N1)pdm09 infection
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Hwajung Yi , Mi-Seon Lee , Joo-Yeon Lee , Hae Kyung Lee , Chun Kang
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J. Microbiol. 2015;53(2):166-175. Published online January 28, 2015
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DOI: https://doi.org/10.1007/s12275-015-4642-2
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Since the 2009 pandemic, monoclonal antibodies (mAbs)
for rapid influenza diagnostic tests (RIDT) have been developed
for specific diagnostics of pandemic viral infection.
Most of the mAbs were poorly characterized because of urgency
during the pandemic. Further characterization of the
mAbs for RIDTs would be beneficial for understanding the
immunological properties of the pandemic virus and utilizing
the mAbs for other research purposes. In this study, it
was confirmed that two mAbs (I38 and D383) in an RIDT for
H1N1pdm09 diagnostics were able to detect H1N1pdm09
virus through enzyme-linked immunosorbent assay (ELISA)
and immunofluorescence assay (IFA). Also, the two mAbs
exhibited reactivity to hemagglutinins (HAs) of both the
H1N1pdm09 and 1918 H1N1 viruses; therefore, the RIDT
using the mAbs could detect HAs of H1N1pdm09 and also
HAs of 1918 H1N1-like strains. In an extension to our previous
study, the epitopes (Sa antigenic site and the interface
area of F?and vestigial esterase subdomains on the HA1 domain
of HA of H1N1pdm09) recognized by the mAbs were
corroborated in depth by IFA with escape-mutants from
the mAbs and mapping of the epitopes on the crystal structure
of human H1N1 viral HAs. Collectively, these results
imply that the mAbs for the RIDT may be suitable for use
in studying the immunological properties of H1N1pdm09
viruses and that the Sa antigenic site and the interface area
between F?and vestigial esterase subdomains on influenza
viral HA recognized by the mAbs are immunologically
conserved regions between H1N1pdm09 and 1918 H1N1.
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Citations
Citations to this article as recorded by  - Antigenic diversity of H5 highly pathogenic avian influenza viruses of clade 2.3.4.4 isolated in Asia
Ayako Ohkawara, Masatoshi Okamatsu, Makoto Ozawa, Duc‐Huy Chu, Lam Thanh Nguyen, Takahiro Hiono, Keita Matsuno, Hiroshi Kida, Yoshihiro Sakoda
Microbiology and Immunology.2017; 61(5): 149. CrossRef
- Development and Evaluation of Multiplex Real-time RT-PCR Assays for Seasonal, Pandemic A/H1pdm09 and Avian A/H5 Influenza Viruses Detection
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Jang-Hoon Choi , Mi-Seon Kim , Joo-Yeon Lee , Nam-Joo Lee , Donghyok Kwon , Min Gu Kang , Chun Kang
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J. Microbiol. 2014;52(6):534-534.
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DOI: https://doi.org/10.1007/s12275-014-0699-6
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Abstract
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In the article by Choi et al. published in Journal of Microbiology 2013; 51, 252-257. Table 1 on page 253 should be changed as below. (Please check attached file)
- Development and Evaluation of Multiplex Real-time RT-PCR Assays for Seasonal, Pandemic A/H1pdm09 and Avian A/H5 Influenza Viruses Detection
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Jang-Hoon Choi , Mi-Seon Kim , Joo-Yeon Lee , Nam-Joo Lee , Donghyok Kwon , Min Gu Kang , Chun Kang
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J. Microbiol. 2013;51(2):252-257. Published online April 27, 2013
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DOI: https://doi.org/10.1007/s12275-013-2452-y
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Since the pandemic influenza A (H1N1) 2009 ((H1N1)pdm09) virus spread all over the world, the (H1N1)pdm09 virus has been circulating with seasonal influenza viruses. We developed rapid and sensitive one-step multiplex real-time RTPCR assays (rRT-PCR) for simultaneous detection of influenza viruses currently circulating in humans, and the avian A/H5 virus. The detection limit of each assay was 4.8 to 1 copies per reaction and no cross-reactivity with other major respiratory pathogens was found. Analytical positive predictive value (PPV), negative predictive value (NPV) sensitivity
and specificity were 100%, 94.1%, 93.7% and 100%, respectively. Clinical evaluation revealed that 1,976 (16.5%) of 11,963 throat swabs from patients with respiratory symptoms were confirmed as 1,651 (83.6%) A/H1pdm09, 308 (15.6%) A/H3 and 17 (0.8%) B virus during the 2010-2011 influenza season. Collectively, the multiplex rRT-PCR assays described here provide a practical tool for reliable implementation
of influenza surveillance and diagnosis.
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Citations
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Heui Man Kim, Namjoo Lee, Mi-Seon Kim, Chun Kang, Yoon-Seok Chung
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- Replication and Pathogenesis of the Pandemic (H1N1) 2009 Influenza Virus in Mammalian Models
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Donghyok Kwon , Kyeongcheol Shin , Seungtae Kim , Yooncheol Ha , Jang-Hoon Choi , Jeong Seon Yang , Joo-Yeon Lee , Chanhee Chae , Hee-Bok Oh , Chun Kang
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J. Microbiol. 2010;48(5):657-662. Published online November 3, 2010
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DOI: https://doi.org/10.1007/s12275-010-0120-z
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660
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This study aimed to characterize the replication and pathogenic properties of a Korean pandemic (H1N1) 2009 influenza virus isolate in ferrets and mice. Ferrets infected with A/Korea/01/2009 (H1N1) virus showed mild clinical signs. The virus replicated well in lungs and slightly in brains with no replication in any other organs. Severe bronchopneumonia and thickening of alveolar walls were detected in the lungs. Viral antigens were detected in the bronchiolar epithelial cells, in peribronchial glands with severe peribronchitis and in cells present in the alveoli. A/Korea/01/2009 (H1N1) virus-infected mice showed weight loss and pathological lung lesions including perivascular cuffing, interstitial pneumonia and alveolitis. The virus replicated highly in the lungs and slightly in the nasal tissues. Viral antigens were detected in bronchiolar epithelial cells, pneumocytes and interstitial macrophages. However, seasonal H1N1 influenza virus did not replicate in the lungs of ferrets, and viral antigens were not detected. Thus, this Korean pandemic (H1N1) 2009 isolate infected the lungs of ferrets and mice successfully and caused more pathological lesions than did the seasonal influenza virus.
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Citations
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Yu-Na Lee, Dong-Hun Lee, Jae-Keun Park, Seong-Su Yuk, Jung-Hoon Kwon, Sang-Soep Nahm, Joong-Bok Lee, Seung-Yong Park, In-Soo Choi, Chang-Seon Song
Journal of General Virology
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Jin Il Kim, Sehee Park, Sangmoo Lee, Ilseob Lee, Jun Heo, Min-Woong Hwang, Joon-Yong Bae, Donghwan Kim, Seok-Il Jang, Mee Sook Park, Man-Seong Park
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Ariel Rodriguez, Ana Falcon, Maria Teresa Cuevas, Francisco Pozo, Susana Guerra, Blanca García-Barreno, Pamela Martinez-Orellana, Pilar Pérez-Breña, Maria Montoya, Jose Antonio Melero, Manuel Pizarro, Juan Ortin, Inmaculada Casas, Amelia Nieto, Andreas Zi
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Y.-N. Lee, D.-H. Lee, J.-K. Park, S.-S. Yuk, J.-H. Kwon, S.-S. Nahm, J.-B. Lee, S.-Y. Park, I.-S. Choi, C.-S. Song
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Dat Tran, Wendy Vaudry, Dorothy L. Moore, Julie A. Bettinger, Scott A. Halperin, David W. Scheifele, Samina Aziz
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Sehee Park, Jin Il Kim, Ilseob Lee, Sangmoo Lee, Min-Woong Hwang, Joon-Yong Bae, Jun Heo, Eun-Joo Lim, Won-Seok Seok, Hee Jin Cheong, Joon Young Song, Woo Joo Kim, Man-Seong Park
Journal of Microbiology.2012; 50(6): 1067. CrossRef - Influenza A(H1N1)pdm09 Virus Suppresses RIG-I Initiated Innate Antiviral Responses in the Human Lung
Wenxin Wu, Wei Zhang, J. Leland Booth, Jordan P. Metcalf, Michael CW. Chan
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- Molecular Characterization and Phylogenetic Analysis of H3N2 Human Influenza A Viruses in Cheongju, South Korea
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Yun Hee Baek , Jeung Hyun Park , Young Jun Song , Min-Suk Song , Philippe Noriel Q. Pascua , Yoon-Soo Hahn , Heon-Seok Han , Ok-Jun Lee , Ki-Soon Kim , Chun Kang , Young-Ki Choi
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J. Microbiol. 2009;47(1):91-100. Published online February 20, 2009
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DOI: https://doi.org/10.1007/s12275-008-0207-y
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To investigate the genetic characteristics of human influenza viruses circulating in Chungbuk province, we tested 510 clinical samples of nasopharyngeal suction from pediatric patients diagnosed with respiratory illness between June 2007 and June 2008. Genetic characterization of the HA genes of H3N2 isolates indicated the relative higher similarity to A/Virginia/04/07 (99.6%) rather than that of A/Wisconsin/67/2005 (98.4%), a Northern Hemisphere 2007~2008 vaccine strain, based on amino acid sequences. We found several altered amino acids at the H3 HA1 antigenic sites compared with the vaccine strain; K140I at site A, K158R at site B, and K173N (H471) or K173Q, and S262N at site E, but there was no antigenic shift among the H3N2 viruses. Interestingly, A/Cheongju/H383/08 and A/Cheongju/H407/08 isolates had single amino acid substitution at D151G on the catalytic site of the N2 NA while A/Cheongju/H412/08 and A/Cheongju/H398/07 isolates had one amino acid deletion at residue 146. Furthermore, we found that 25% (3 out of 12 isolates) of the H3N2 subtype viruses had the amino acid substitution at position 31 on the M2 protein (Aspartic acid to Asparagine) and confirmed their drug-resistance by biological assays. Taken together, the results of this study demonstrated continuous evolutions of human H3N2 viruses by antigenic drift and also highlighted the need to closely monitor antigenic drug resistance in influenza A viruses to aid in the early detection of potentially pandemic strains, as well as underscore the need for new therapeutics.
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