- Inhibitory effects of bee venom and its components against viruses in vitro and in vivo
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Md Bashir Uddin , Byeong-Hoon Lee , Chamilani Nikapitiya , Jae-Hoon Kim , Tae-Hwan Kim , Hyun-Cheol Lee , Choul Goo Kim , Jong-Soo Lee , Chul-Joong Kim
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J. Microbiol. 2017;55(2):160-160.
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DOI: https://doi.org/10.1007/s12275-017-0668-y
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Abstract
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In the article by Uddin et al. published in Journal of Microbiology 2016; 54, 853–866, the Protocol number on page 856 and 865 should be corrected as below.
- Inhibitory effects of bee venom and its components against viruses in vitro and in vivo
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Md Bashir Uddin , Byeong-Hoon Lee , Chamilani Nikapitiya , Jae-Hoon Kim , Tae-Hwan Kim , Hyun-Cheol Lee , Choul Goo Kim , Jong-Soo Lee , Chul-Joong Kim
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J. Microbiol. 2016;54(12):853-866. Published online November 26, 2016
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DOI: https://doi.org/10.1007/s12275-016-6376-1
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Abstract
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Bee venom (BV) from honey bee (Apis Melifera L.) contains
at least 18 pharmacologically active components including
melittin (MLT), phospholipase A2 (PLA2), and apamin etc.
BV is safe for human treatments dose dependently and proven
to possess different healing properties including antibacterial
and antiparasitidal properties. Nevertheless, antiviral
properties of BV have not well investigated. Hence, we
identified the potential antiviral properties of BV and its
component against a broad panel of viruses. Co-incubation
of non-cytotoxic amounts of BV and MLT, the main component
of BV, significantly inhibited the replication of enveloped
viruses such as Influenza A virus (PR8), Vesicular
Stomatitis Virus (VSV), Respiratory Syncytial Virus (RSV),
and Herpes Simplex Virus (HSV). Additionally, BV and MLT
also inhibited the replication of non-enveloped viruses such
as Enterovirus-71 (EV-71) and Coxsackie Virus (H3). Such
antiviral properties were mainly explained by virucidal mechanism.
Moreover, MLT protected mice which were challenged
with lethal doses of pathogenic influenza A H1N1
viruses. Therefore, these results provides the evidence that BV
and MLT could be a potential source as a promising antiviral
agent, especially to develop as a broad spectrum antiviral
agent.
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Citations
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- Molecular characterization of mammalian-adapted Korean-type avian H9N2 virus and evaluation of its virulence in mice
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Kuk Jin Park , Min-Suk Song , Eun-Ha Kim , Hyeok-il Kwon , Yun Hee Baek , Eun-hye Choi , Su-Jin Park , Se Mi Kim , Young-il Kim , Won-Suk Choi , Dae-Won Yoo , Chul-Joong Kim , Young Ki Choi
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J. Microbiol. 2015;53(8):570-577. Published online July 31, 2015
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DOI: https://doi.org/10.1007/s12275-015-5329-4
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Abstract
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Avian influenza A virus (AIV) is commonly isolated from
domestic poultry and wild migratory birds, and the H9N2
subtype is the most prevalent and the major cause of severe
disease in poultry in Korea. In addition to the veterinary concerns
regarding the H9N2 subtype, it is also considered to
be the next potential human pandemic strain due to its rapid
evolution and interspecies transmission. In this study, we
utilize serial lung-to-lung passage of a low pathogenic avian
influenza virus (LPAI) H9N2 (A/Ck/Korea/163/04, WT163)
(Y439-lineage) in mice to increase pathogenicity and investigate
the potential virulence marker. Mouse-adapted H9N2
virus obtained high virulence (100% mortality) in mice after
98 serial passages. Sequence results show that the mouse
adaptation (ma163) possesses several mutations within seven
gene segments (PB2, PA, HA, NP, NA, M, and NS) relative
to the wild-type strain. The HA gene showed the most mutations
(at least 11) with one resulting in the loss of an N-glycosylation
site (at amino acid 166). Moreover, reverse genetic
studies established that an E627K substitution in PB2 and the
loss of the N-glycosylation site in the HA protein (aa166) are
critical virulence markers in the mouse-adapted H9N2 virus.
Thus, these results add to the increasing body of mutational
analysis data defining the function of the viral polymerase
and HA genes and their roles in mammalian host adaptation.
To our knowledge, this is first report of the generation
of a mammalian-adapted Korea H9N2 virus (Y493-lineages).
Therefore, this study offers valuable insights into the molecular
evolution of the LPAI Korean H9N2 in a new host and
adds to the current knowledge of the molecular markers associated
with increased virulence.
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Citations
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- Evaluation of the Efficacy of a Pre-pandemic H5N1 Vaccine (MG1109) in Mouse and Ferret Models
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Min-Suk Song , Ho-Jin Moon , Hyeok-il Kwon , Philippe Noriel Q. Pascua , Jun Han Lee , Yun Hee Baek , Kyu-Jin Woo , Juhee Choi , Sangho Lee , Hyunseung Yoo , In gyeong Oh , Yeup Yoon , Jong-Bok Rho , Moon-Hee Sung , Seung-Pyo Hong , Chul-Joong Kim , Young Ki Choi
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J. Microbiol. 2012;50(4):715-715.
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Abstract
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In the article by Song et al. that appears in the Journal of Microbiology 2012; 50, 478-488. Page 478, the name of 7th author, Kyu-Jin Woo, should read as Gyu-Jin Woo.
- Evaluation of the Efficacy of a Pre-pandemic H5N1 Vaccine (MG1109) in Mouse and Ferret Models
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Min-Suk Song , Ho-Jin Moon , Hyeok-il Kwon , Philippe Noriel Q. Pascua , Jun Han Lee , Yun Hee Baek , Kyu-Jin Woo , Juhee Choi , Sangho Lee , Hyunseung Yoo , In gyeong Oh , Yeup Yoon , Jong-Bok Rho , Moon-Hee Sung , Seung-Pyo Hong , Chul-Joong Kim , Young Ki Choi
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J. Microbiol. 2012;50(3):487-488.
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Abstract
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The threat of a highly pathogenic avian influenza (HPAI) H5N1 virus causing the next pandemic remains a major concern. In this study, we evaluated the immunogenicity and efficacy of an inactivated whole-virus H5N1 pre-pandemic vaccine (MG1109) formulated by Green Cross Co., Ltd containing the hemagglutinin (HA) and neuraminidase (NA) genes of the clade 1 A/Vietnam/1194/04 virus in the backbone of A/Puerto Rico/8/34 (RgVietNam/04xPR8/34). Administration of the MG1109 vaccine (2-doses) in mice and ferrets elicited high HI and SN titers in a dose-dependent manner against the homologous (RgVietNam/04xPR8/34) and various heterologous H5N1 strains, (RgKor/W149/06xPR8/34, RgCambodia/04xPR8/34, RgGuangxi/05xPR8/34), including a heterosubtypic H5N2 (A/Aquatic bird/orea/W81/05) virus. However, efficient cross-reactivity was not observed against heterosubtypic H9N2 (A/Ck/Korea/H0802/08) and H1N1 (PR/8/34) viruses. Mice immunized with 1.9 μg HA/dose of MG1109 were completely protected from lethal challenge with heterologous wild-type HPAI H5N1 A/EM/Korea/W149/06 (clade 2.2) and mouse-adapted H5N2 viruses. Furthermore, ferrets administered at least 3.8 μg HA/dose efficiently suppressed virus growth in the upper respiratory tract and lungs. Vaccinated mice and ferrets also demonstrated attenuation of clinical disease signs and limited virus spread to other organs. Thus, this vaccine provided immunogenic responses in mouse and ferret models even against challenge with heterologous HPAI H5N1 and H5N2 viruses. Since the specific strain of HPAI H5N1 virus that would potentially cause the next outbreak is unknown, pre-pandemic vaccine preparation that could provide crossprotection against various H5 strains could be a useful approach in the selection of promising candidate vaccines in the future.
- Production and Prophylactic Efficacy Study of Human Papillomavirus-like Particle Expressing HPV16 L1 Capsid Protein
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Jie-Yun Park , Hyun-Mi Pyo , Sun-Woo Yoon , Sun-Young Baek , Sue-Nie Park , Chul-Joong Kim , Haryoung Poo
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J. Microbiol. 2002;40(4):313-318.
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Abstract
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To perform the prophylactic study of a vaccine derived from human papillomavirus (HPV) using Balb/c mice, we produced virus like particles consisting of HPV capsid protein L1 which has been reported to induce significant humoral and cellular immunity using various animal model systems. In order to produce HPV16 VLPs, the cDNA of L1 capsid protein in HPV type 16, obtained by polymerase chain reaction, was inserted into yeast expression vector, YEG[alpha]-HIR525 under the control of GAL10 promoter. The transformation of YEG[alpha]-HPV16 L1 was performed into the yeast Saccharomyces cerevisiae Y2805 by the lithium acetate method and the yeast clone expressing the highest level of L1 capsid protein of human papillomavirus type 16 was selected by Western blot analysis using anti-HPV16 L1 antibody. The purification of HPV16 VLP has been performed by the ultracentrifugation and gel-filtration methods. To validate the vaccine efficacy of the purified HPV16 VLPs and investigate the properties of HPV16 VLPs to induce humoral immunity, ELISA assay was performed. A significantly increased production of anti-HPV16 VLP antibodies was observed in sera from immunized mice. The neutralization activity of antibodies in the sera from the vaccinated mice was demonstrated by a rapid and simple assay to detect hemagglutihation inhibition activity
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