- Enhancement of Anti-candidal Activity of Endophytic Fungus Phomopsis sp. ED2, Isolated from Orthosiphon stamineus Benth, by Incorporation of Host Plant Extract in Culture Medium
-
Tong Woei Yenn , Chong Chai Lee , Darah Ibrahim , Latiffah Zakaria
-
J. Microbiol. 2012;50(4):581-585. Published online July 21, 2012
-
DOI: https://doi.org/10.1007/s12275-012-2083-8
-
-
762
View
-
0
Download
-
17
Crossref
-
 Abstract
 PDF
-
This study examined the effect of host extract in the culture medium on anti-candidal activity of Phomopsis sp. ED2, previously isolated from the medicinal herb Orthosiphon stamineus Benth. Interestingly, upon addition of aqueous host extract to the culture medium, the ethyl acetate extract prepared from fermentative broth exhibited moderate anticandidal activity in a disc diffusion assay. The minimal inhibitory concentration of this extract was 62.5 μg/ml and it only exhibited fungistatic activity against C. albicans. In the time-kill study, a 50% growth reduction of C. albicans was observed at 31.4 h for extract from the culture incorporating host extract. In the bioautography assay, only one single spot (Rf 0.59) developed from the extract exhibited anti-candidal activity. A spot with the a similar Rf was not detected for the crude extract from YES broth without host extract. This indicated that the terpenoid anti-candidal compound was only produced when the host extract was introduced into the medium. The study concluded that the incorporation of aqueous extract of the host plant into the culture medium significantly enhanced the anti-candidal activity of Phomopsis sp. ED2.
-
Citations
Citations to this article as recorded by  - Fungal endophytes and their secondary metabolites for crop protection
Jorge Rojas López-Menchero, Fotios A. Lyssaios, Nicolás Reyes-Castillo, Maria Fe Andres, Azucena González-Coloma, Carmen E. Díaz
Journal of Pest Science.2026;[Epub] CrossRef - Antibacterial potential of epiphytic fungi obtained from brown algae of Kenyan coastal waters
Aragaw Zemene Sendekie, Kimang’a Andrew Nyerere, Purity Kinya Kaaria, Guadalupe Virginia Nevárez-Moorillón
PLOS One.2026; 21(4): e0346865. CrossRef - Assessment of antibacterial and anti-biofilm activities of marine fungal epiphytes associated with red and green algae of the Kenyan coast
Aragaw Zemene Sendekie, Kimang'a Andrew Nyerere, Purity Kinya Kaaria
Biotechnology Notes.2026; 7: 120. CrossRef - Biocontrol Screening of Endophytes: Applications and Limitations
Nikhil Kashyap, Sandeep Kumar Singh, Nisha Yadav, Vipin Kumar Singh, Madhuree Kumari, Dharmendra Kumar, Livleen Shukla, Kaushalendra, Nikunj Bhardwaj, Ajay Kumar
Plants.2023; 12(13): 2480. CrossRef -
Bioactive steroids from seed germination supporting fungus (
Ceratobasidium
GS2) of the terrestrial orchid
Gymnadenia conopsea
Lixin Shi, Zeyu Zhao, Luna Yang, Gang Ding, Xiaoke Xing
Mycology.2023; 14(4): 371. CrossRef - Bioactivity study and metabolic profiling of Colletotrichum alatae LCS1, an endophyte of club moss Lycopodium clavatum L.
Hiran Kanti Santra, Debdulal Banerjee, Guadalupe Virginia Nevárez-Moorillón
PLOS ONE.2022; 17(4): e0267302. CrossRef - Effect of Culture Medium Incorporated with Ocimum sanctum Extract in Enhancing Anti-MRSA activity of Endophytic Fungus, Lasiodiplodia pseudotheobromae
Mohd Taufiq Mat Jalil, Nurul Aili Zakaria, Darah Ibrahim
Journal of Pure and Applied Microbiology.2021; 15(3): 1398. CrossRef - Chemometric window to antimicrobial activity of biomolecules isolated from endophytic fungi
Biljana Otašević, Jovana Krmar, Nevena Đajić, Janko Ignjatović, Ana Protić
Arhiv za farmaciju.2020; 70(3): 142. CrossRef -
Muscodor sp. IBRL OS-94, A Promising Endophytic Fungus of Ocimum sanctum with Antimicrobial Activity
Mohd Taufiq Mat Jalil, Nabila Husna Hairudin, Darah Ibrahim
Pharmaceutical Sciences.2020; 27(2): 268. CrossRef - Sustained Release Geraniol Nanoparticles Inhibit Human Axillary Odor-Causing Bacteria
Tan Wen Nee, Leong Chean Ring, Venoth Arumugam, Judy Loo Ching Yee, Lee Wing Hin, Fahmi Asyadi Md Yusof, Mohd Azizan Mohd Noor, Tong Woei Yenn
Arabian Journal for Science and Engineering.2019; 44(1): 103. CrossRef - Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System
M.M.J. Taufiq, I. Darah
Journal of Pure and Applied Microbiology.2019; 13(4): 2517. CrossRef - Fungal endophytes isolated from the leaves of a medicinal plant, Ocimum sanctum Linn and evaluation of their antimicrobial activities
M. M. J. Taufiq, I. Darah
African Journal of Microbiology Research.2018; 12(26): 616. CrossRef - Antimicrobial activity of endophytic fungi from olive tree leaves
Cynthia Malhadas, Ricardo Malheiro, José Alberto Pereira, Paula Guedes de Pinho, Paula Baptista
World Journal of Microbiology and Biotechnology.2017;[Epub] CrossRef - Anticandidal effect of endophytic bacteria isolated from Equisetum arvense L. against Candida albicans and Candida glabrata
Gitishree Das, Jayanta Kumar Patra, Jaehyuk Choi, Kwang-Hyun Baek
Brazilian Archives of Biology and Technology.2017;[Epub] CrossRef - Bioactive Compound and Therapeutic Value of the Some Malaysia Medicinal Plants: A Review
Nik Nurnaeimah Nik Muhamm, Mohammad Moneruzzama Khandaker, Nashriyah Mat
Journal of Agronomy.2015; 14(4): 319. CrossRef - Molecular characterization of an endophytic Phomopsisliquidambaris CBR-15 from Cryptolepis buchanani Roem. and impact of culture media on biosynthesis of antimicrobial metabolites
H. C. Yashavantha Rao, Parthasarathy Santosh, Devaraju Rakshith, Sreedharamurthy Satish
3 Biotech.2015; 5(2): 165. CrossRef - Antimicrobial Activity of Endophytic Fungi Isolated from Swietenia macrophylla Leaves
Darah Ibrahim, Chong Chai Lee, Lim Sheh-Hong
Natural Product Communications.2014;[Epub] CrossRef
|
MSK
