- Diversity of lactic acid bacteria from Miang, a traditional fermented tea leaf in northern Thailand and their tannin-tolerant ability in tea extract
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Siriporn Chaikaew , Sasitorn Baipong , Teruo Sone , Apinun Kanpiengjai , Naradorn Chui-chai , Kozo Asano , Chartchai Khanongnuch
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J. Microbiol. 2017;55(9):720-729. Published online September 2, 2017
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DOI: https://doi.org/10.1007/s12275-017-7195-8
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The microbiota of lactic acid bacteria (LAB) in thirty-five
samples of Miang, a traditional fermented tea leaf product,
collected from twenty-two different regions of eight provinces
in upper northern Thailand was revealed through the
culture-dependent technique. A total of 311 presumptive
LAB strains were isolated and subjected to clustering analysis
based on repetitive genomic element-PCR (rep-PCR) fingerprinting
profiles. The majority of the strains belonged to
the Lactobacillus genera with an overwhelming predominance
of the Lb. plantarum group. Further studies of species-specific
PCR showed that 201 of 252 isolates in the Lb. plantarum
group were Lb. plantarum which were thus considered
as the predominant LAB in Miang, while the other 51 isolates
belonged to Lb. pentosus. In contrast to Lb. plantarum,
there is a lack of information on the tannase gene and the
tea tannin-tolerant ability of Lb. pentosus. Of the 51 Lb. pentosus
isolates, 33 were found to harbor the genes encoding
tannase and shared 93-99% amino acid identity with tannase
obtained from Lb. pentosus ATCC 8041T. Among 33
tannase gene-positive isolates, 23 isolates exhibited high tannin-
tolerant capabilities when cultivated on de Man Rogosa
and Sharpe agar-containing bromocresol purple (0.02 g/L,
MRS-BCP) supplemented with 20% (v/v) crude tea extract,
which corresponded to 2.5% (w/v) tannins. These Lb. pentosus
isolates with high tannin-tolerant capacity are expected
to be the high potential strains for functional tannase production involved in Miang fermentation as they will bring
about certain benefits and could be used to improve the
fermentation of tea products.
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DOI: https://doi.org/10.1007/s12275-012-1108-7
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839
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Abstract
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Lactic acid bacteria from healthy breast-fed infants were
isolated and screened for β-galactosidase production in
MRS broth. Among 49 isolates that exhibited the yellow
clear zone on MRS agar supplemented with bromocresol
blue, the isolate CM33 was selected as being the highest
β-galactosidase producer and was identified as Lactobacillus
fermentum based on its morphological characteristics and
16S rDNA nucleotide sequence. L. fermentum CM33 exhibited
a good survival rate under the simulated stomach
passage model, comparable to known probiotic strains L.
gallinarum JCM2011 and L. agilis JCM1187. L. fermentum
CM33 was antagonistic to pathogenic bacteria Listeria monocytogenes,
Escherichia coli 0157:H7, Salmonella typhi, and
Salmonella enteriditis, using the well diffusion method. In
addition, the selected lactobacilli exhibited a high growth
rate when cultivated in modified MRS containing commercial
galactooligosaccharide (GOS) as a sole carbon source,
as well as in glucose. A preliminary study on the enzymatic
synthesis of oligosaccharide using crude β-galactosidase revealed
the capability for oligosaccharide synthesis by the
transgalactosylation activity.
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