Journal of Microbiology

Cha-Yeon Kim

1 Article

Isolation of Synthetic Lethal Mutations in the rsm1-null Mutant of Fission Yeast: DongGeRaMi Moon , Yun-Sun Park , Cha-Yeon Kim , Jin Ho Yoon; J. Microbiol. 2010;48(5):701-705. Published online November 3, 2010; DOI: https://doi.org/10.1007/s12275-010-0353-x

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To identify mutations in genes that are genetically linked to rsm1, we performed a synthetic lethal genetic screen in the fission yeast, Schizosaccharomyces pombe. Four mutations that showed synthetic lethality in combination with the rsm1null allele were isolated from approximately 320,000 colonies and defined in three complementation groups. One mutant (SLrsm1) exhibited a significant accumulation of poly(A)+ RNA in the nucleus under synthetic lethal conditions, while the rest had no mRNA export defects. In addition, some genes (spmex67, rae1, or mlo3) required for mRNA export complemented the growth defects of the identified mutants. These results suggest that the isolated mutants contain mutations in genes that are involved in mRNA export and/or pre-mRNA retention.

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Structure of the pre-mRNA leakage 39-kDa protein reveals a single domain of integrated zf-C3HC and Rsm1 modules
Hideharu Hashimoto, Daniel H. Ramirez, Ophélie Lautier, Natalie Pawlak, Günter Blobel, Benoît Palancade, Erik W. Debler
Scientific Reports.2022;[Epub] CrossRef
Effects of spThoc7 Deletion on Growth and mRNA Export in Fission Yeast
Eun-Jin Koh, Jin Ho Yoon
The Korean Journal of Microbiology.2014; 50(3): 249. CrossRef

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