- Potential Use of Mycobacterium paragordonae for Antimycobacterial Drug Screening Systems
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Ga-Yeong Cha , Hyejun Seo , Jaehun Oh , Byoung-Jun Kim , Bum-Joon Kim
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J. Microbiol. 2023;61(1):121-129. Published online January 31, 2023
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DOI: https://doi.org/10.1007/s12275-022-00009-1
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Our recent genome-based study indicated that Mycobacterium paragordonae (Mpg) has evolved to become more adapted to
an intracellular lifestyle within free-living environmental amoeba and its enhanced intracellular survival within Acanthamoeba
castellanii was also proved. Here, we sought to investigate potential use of Mpg for antimycobacterial drug screening
systems. Our data showed that Mpg is more susceptible to various antibiotics compared to the close species M. marinum
(Mmar) and M. gordonae, further supporting its intracellular lifestyle in environments, which would explain its protection
from environmental insults. In addition, we developed two bacterial whole-cell-based drug screening systems using a
recombinant Mpg stain harboring a luciferase reporter vector (rMpg-LuxG13): one for direct application to rMpg-LuxG13
and the other for drug screening via the interaction of rMpg-LuxG13 with A. castellanii. Direct application to rMpg-LuxG13
showed lower inhibitory concentration 50 (
IC50) values of rifampin, isoniazid, clarithromycin, and ciprofloxacin against
Mpg compared to Mmar. Application of drug screening system via the interaction of rMpg-LuxG13 with A. castellanii also
exhibited lower IC50
values for rifampin against Mpg compared to Mmar. In conclusion, our data indicate that Mpg is more
susceptible to various antibiotics than other strains. In addition, our data also demonstrate the feasibility of two whole cellbased
drug screening systems using rMpg-LuxG13 strain for the discovery of novel anti-mycobacterial drugs.
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Citations
Citations to this article as recorded by  -
6-methylflavone exerts selective antimycobacterial activity with membrane energetics disruption and demonstrates therapeutic potential against
Mycobacterium abscessus
infection
Jaehun Oh, Jeong-Won Shin, Hyejun Seo, Hyung-Jun Kim, Nam-Jung Kim, Bum-Joon Kim, Prabagaran Narayanasamy
Microbiology Spectrum.2026;[Epub] CrossRef - Mycobacterium paragordonae: Insights into its Research Progress and Potential Applications
Hyejun Seo, Ju-Young Lee, Bum-Joon Kim
Journal of Bacteriology and Virology.2024; 54(4): 273. CrossRef - Protection against tuberculosis achieved by dissolving microneedle patches loaded with live Mycobacterium paragordonae in a BCG prime-boost strategy
Mi-Hyun Lee, Hyejun Seo, Moon-Su Lee, Byoung Jun Kim, Hye Lin Kim, Du Hyung Lee, Jaehun Oh, Ju Yeop Shin, Ju Young Jin, Do Hyeon Jeong, Bum-Joon Kim
Frontiers in Immunology.2023;[Epub] CrossRef
- COVID-19 vaccine development based on recombinant viral and bacterial vector systems: combinatorial effect of adaptive and trained immunity
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Mi-Hyun Lee , Bum-Joon Kim
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J. Microbiol. 2022;60(3):321-334. Published online February 14, 2022
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DOI: https://doi.org/10.1007/s12275-022-1621-2
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1,058
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Severe acute respiratory syndrome coronavirus 2 virus (SARSCoV-
2) infection, which causes coronavirus disease 2019
(COVID-19), has led to many cases and deaths worldwide.
Therefore, a number of vaccine candidates have been developed
to control the COVID-19 pandemic. Of these, to date,
21 vaccines have received emergency approval for human
use in at least one country. However, the recent global emergence
of SARS-CoV-2 variants has compromised the efficacy
of the currently available vaccines. To protect against these
variants, the use of vaccines that modulate T cell-mediated
immune responses or innate immune cell memory function,
termed trained immunity, is needed. The major advantage of
a vaccine that uses bacteria or viral systems for the delivery of
COVID-19 antigens is the ability to induce both T cell-mediated
and humoral immune responses. In addition, such
vaccine systems can also exert off-target effects via the vector
itself, mediated partly through trained immunity; compared
to other vaccine platforms, suggesting that this approach can
provide better protection against even vaccine escape variants.
This review presents the current status of the development of
COVID-19 vaccines based on recombinant viral and bacterial
delivery systems. We also discuss the current status of the
use of licensed live vaccines for other infections, including
BCG, oral polio and MMR vaccines, to prevent COVID-19
infections.
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- Effect of amikacin on cell wall glycopeptidolipid synthesis in Mycobacterium abscessus
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So-Young Lee , Hee-Youn Kim , Byoung-Jun Kim , Hong Kim , Seung-hyeok Seok , Bum-Joon Kim , Yoon-Hoh Kook
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J. Microbiol. 2017;55(8):640-647. Published online July 28, 2017
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DOI: https://doi.org/10.1007/s12275-017-6503-7
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903
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7
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Cultivation of the smooth colony Mycobacterium abscessus
at the sub-minimum inhibitory concentration (MIC) of amikacin
changed its growth pattern including its colony morphology
(smooth to rough) and cell arrangement (dispersed
to cord formation). In addition, reduced sliding motility and
biofilm formation were observed. The amount of glycogpetidolipid
(GPL) and mRNA expression of key genes involved
in GPL synthesis were decreased in the amikacin-treated M.
abscessus strain. An in vitro infection assay revealed that the
amikacin-treated smooth M. abscessus strain induced more
pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) than that
of the smooth strain in murine macrophage cells. These results
suggest that long-term exposure to a low concentration of
amikacin causes a physical change in the cell wall which may
increase its virulence.
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Bong-Hee Lee , Hong Kim , Hyun-Ju Kim , Yoon-Kyu Lim , Kyung-Hee Byun , Brian Hutchinson , Chang-Jin Kim , Young-Hwan Ko , Keun-Hwa Lee , Chang-Yong Cha , Yoon-Hoh Kook , Bum-Joon Kim
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DOI: https://doi.org/2158 [pii]
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Abstract
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Epilepsy constitutes a significant public health problem, and even the newest drugs and neurosurgical techniques have proven unable to cure the disease. In order to select a group of isolates which could generate an active compound with neuroprotective or antiepileptic properties, we isolated 517 actinomycete strains from soil samples taken from Jeju Island, in South Korea. We then screened these strains for possible anti-apoptotic effects against serum deprivation-induced hippocampal cell death, using the 3-(4, 5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay as an in vitro test. The excitotoxic glutamate analog, kainic acid (KA), was used to induce seizures in experimental mice in our in vivo tests. As a result of this testing, we located one strain which exhibited profound neuroprotective activity. This strain was identified as a Streptomyces species, and exhibited the rifampin-resistant genotype, Asn(AAC)^442, according to the results of 16S rRNA and rpoB gene analyses
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