- An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense
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Lee Pei Lee Angel , Mohd Termizi Yusof , Intan Safinar Ismail , Bonnie Tay Yen Ping , Intan Nur Ainni Mohamed Azni , Norman Hj Kamarudin , Shamala Sundram
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J. Microbiol. 2016;54(11):732-744. Published online October 29, 2016
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DOI: https://doi.org/10.1007/s12275-016-6304-4
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Abstract
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Ganoderma boninense is the causal agent of a devastating disease
affecting oil palm in Southeast Asian countries. Basal
stem rot (BSR) disease slowly rots the base of palms, which
radically reduces productive lifespan of this lucrative crop.
Previous reports have indicated the successful use of Trichoderma
as biological control agent (BCA) against G. boninense
and isolate T. virens 7b was selected based on its initial screening.
This study attempts to decipher the mechanisms responsible
for the inhibition of G. boninense by identifying and
characterizing the chemical compounds as well as the physical
mechanisms by T. virens 7b. Hexane extract of the isolate
gave 62.60% ± 6.41 inhibition against G. boninense and
observation under scanning electron microscope (SEM) detected
severe mycelial deformation of the pathogen at the
region of inhibition. Similar mycelia deformation of G. boninense
was observed with a fungicide treatment, Benlate®
indicating comparable fungicidal effect by T. virens 7b. Fraction
4 and 5 of hexane active fractions through preparative
thin layer chromatography (P-TLC) was identified giving the
best inhibition of the pathogen. These fractions comprised of
ketones, alcohols, aldehydes, lactones, sesquiterpenes, monoterpenes,
sulphides, and free fatty acids profiled through gas
chromatography mass spectrometry detector (GC/MSD). A
novel antifungal compound discovery of phenylethyl alcohol
(PEA) by T. virens 7b is reported through this study. T.
virens 7b also proved to be an active siderophore producer
through chrome azurol S (CAS) agar assay. The study demonstrated
the possible mechanisms involved and responsible
in the successful inhibition of G. boninense.
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