- Taxonomic description and genome sequence of Halobacillus marinus sp. nov., a novel strain isolated from Chilika Lake, India
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Ananta N. Panda , Samir Ranjan Mishra , Lopamudra Ray , Surajit Das , Gurdeep Rastogi , Tapan Kumar Adhya , Mrutyunjay Suar , Vishakha Raina
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J. Microbiol. 2018;56(4):223-230. Published online April 2, 2018
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DOI: https://doi.org/10.1007/s12275-018-7387-x
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A moderately halophilic spore forming, motile, Gram-positive,
rod-shaped bacterial strain designated as KGW1T was
isolated from water sample of Chilika Lake and characterized
taxonomically using polyphasic approach. The strain grew
in the presence of 0–25% (w/v) NaCl in marine salt agar media,
hydrolyzes casein, and gelatin and shows presence of alkaline
proteases. The major cell wall menaquinone was MK7
and major cellular fatty acids were anteiso-C15:0 (44.89%),
anteiso-C17:0 (6.18%), isoC15:0 (19.38%), and iso-C16:0 (7.39%).
Several chemotaxonomic features conform the isolate be a
member of genus Halobacillus. The isolate KGW1T contained
A1γ meso-Dpm-direct type of peptidoglycan which is different
from its phylogenetically closest neighbours. The 16S
rRNA gene sequence based phylogenetic analysis also revealed
the strain KGW1T was affiliated to the genus Halobacillus
and sequence similarity between the isolated strain
and the type strains of Halobacillus species were found closest
to, H. dabanensis D-8 DSM 18199T (99.08%) and H. faecis
IGA7-4 DSM 21559T (99.01%), H. trueperi SL-5 DSM 10404T
(98.94%). The in silico DDH showed that the values in a range
of 14.2–17.5% with the most closest strain H. dabanensis D-8
DSM 18199T and other type strains of the genus Halobacillus
for which whole genome sequence is reported. DNA-DNA
relatedness between strain KGW1T and the closest type strain
Halobacillus trueperi DSM 10404T was 11.75% (± 1.15). The
draft genome sequence includes 3,683,819 bases and comprises
of 3898 predicted coding sequences with a G + C content
of 46.98%. Thus, the significant distinctiveness supported
by phenotypic and genotypic data with its closest neighbors
and other closely related species confirm the strain KGW1T
to be classified as a novel species within the genus Halobacillus,
for which the name Halobacillus marinus sp. nov. is
proposed. The type strain is KGW1T (= DSM 29522 = JCM
30443).
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