- Two novel synthetic peptides inhibit quorum sensing-dependent biofilm formation and some virulence factors in Pseudomonas aeruginosa PAO1
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Mostafa N. Taha , Amal E. Saafan , A. Ahmedy , Eman El Gebaly , Ahmed S. Khairalla
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J. Microbiol. 2019;57(7):618-625. Published online June 27, 2019
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DOI: https://doi.org/10.1007/s12275-019-8548-2
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Quorum sensing (QS) regulates virulence factor expression
in Pseudomonas aeruginosa. Inhibiting the QS-controlled virulence
factors without inhibiting the growth of P. aeruginosa
is a promising approach for overcoming the widespread
resistance of P. aeruginosa. This study was proposed to investigate
the effects of two novel synthetic peptides on the biofilm
development and virulence factor production of P. aeruginosa.
The tested strain was P. aeruginosa PAO1. The results
indicated that both of the synthetic peptides (LIVRHK and
LIVRRK) inhibited (P < 0.05) the formation of biofilms and
the production of virulence factors, including pyocyanin, protease,
and rhamnolipids, without inhibiting the growth of
PAO1. Additionally, we detected transcriptional changes related
to QS and found a significant reduction in the levels of
gene expression of lasI, lasR, rhlI, and rhlR. This study demonstrates
that LIVRRK and LIVRHK are novel synthetic peptides
that can act as potent inhibitors of QS-regulated virulence
factors in P. aeruginosa. Moreover, these synthetic peptides
have potential applications in the treatment of biofilmrelated
diseases. Both peptides may be able to control chronic
infections and biofilm-associated problems of P. aeruginosa.
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Citations
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Bioorganic & Medicinal Chemistry.2026; 133: 118496. CrossRef - Pseudomonas aeruginosa: Pathogenesis‐Immunity Arm Race, Vaccine and Therapeutics Development Panoramas
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LWT.2025; 218: 117542. CrossRef - Harnessing Chemical Diversity to Disarm Bacterial Communication for 25 Years: Natural Products, Repurposed Drugs, Peptides, and Synthetic Quorum Sensing Inhibitors
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ChemMedChem.2025;[Epub] CrossRef - Anti-QS Strategies Against Pseudomonas aeruginosa Infections
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Microorganisms.2025; 13(8): 1838. CrossRef - Rational Design, Computational Analysis and Antibacterial Activities of Synthesized Peptide-Based Molecules Targeting Quorum Sensing-Dependent Biofilm Formation in Pseudomonas aeruginosa
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Surfaces and Interfaces.2025; 72: 107390. CrossRef - Antibiofilm activity of marine microbial natural products: potential peptide- and polyketide-derived molecules from marine microbes toward targeting biofilm-forming pathogens
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Jiarui Du, Jiahao Li, Juqi Wen, Jun Liu, Haichuan Xiao, Antian Zhang, Dongdong Yang, Pinghua Sun, Haibo Zhou, Jun Xu
International Journal of Molecular Sciences.2024; 25(14): 8000. CrossRef - Antimicrobial peptides fight against Pseudomonas aeruginosa at a sub-inhibitory concentration via anti-QS pathway
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Bioorganic Chemistry.2023; 141: 106922. CrossRef - The Role of Quorum Sensing Molecules in Bacterial–Plant Interactions
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Metabolites.2023; 13(1): 114. CrossRef - Peptide LQLY3-1, a novel Vibrio harveyi quorum sensing inhibitor produced by Lactococcus lactis LY3-1
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LWT.2022; 170: 114093. CrossRef - Design and assessment of novel synthetic peptides to inhibit quorum sensing-dependent biofilm formation in Pseudomonas aeruginosa
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Biofouling.2022; 38(2): 131. CrossRef - Effects of active compounds from Cassia fistula on quorum sensing mediated virulence and biofilm formation in Pseudomonas aeruginosa
Zoya Peerzada, Ashish M. Kanhed, Krutika B. Desai
RSC Advances.2022; 12(24): 15196. CrossRef - Antimicrobial peptides properties beyond growth inhibition and bacterial killing
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PeerJ.2022; 10: e12667. CrossRef - A Bacterial Isolate Capable of Quenching Both Diffusible Signal Factor- and N-Acylhomoserine Lactone-Family Quorum Sensing Signals Shows Much Enhanced Biocontrol Potencies
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Journal of Agricultural and Food Chemistry.2022; 70(25): 7716. CrossRef - Algal polysaccharide’s potential to combat respiratory infections caused by Klebsiella pneumoniae and Serratia marcescens biofilms
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Infection and Drug Resistance.2021; Volume 14: 3637. CrossRef - Molecular engineering of antimicrobial peptides: microbial targets, peptide motifs and translation opportunities
Priscila Cardoso, Hugh Glossop, Thomas G. Meikle, Arturo Aburto-Medina, Charlotte E. Conn, Vijayalekshmi Sarojini, Celine Valery
Biophysical Reviews.2021; 13(1): 35. CrossRef - Type VI secretion system of Pseudomonas aeruginosa is associated with biofilm formation but not environmental adaptation
Lihua Chen, Yaru Zou, Asmaa Abbas Kronfl, Yong Wu
MicrobiologyOpen.2020;[Epub] CrossRef - Synergism between Host Defence Peptides and Antibiotics Against Bacterial Infections
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Current Topics in Medicinal Chemistry.2020; 20(14): 1238. CrossRef - Olive Leaf Extract Modulates Quorum Sensing Genes and Biofilm Formation in Multi-Drug Resistant Pseudomonas aeruginosa
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