- S5 Lipase : An Organic Solvent Tolerant Enzyme
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Raja Noor Zaliha Raja Abdul Rahman , Syarul Nataqain Baharum , Abu Bakar Salleh , Mahiran Basri
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J. Microbiol. 2006;44(6):583-590.
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DOI: https://doi.org/2470 [pii]
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 Abstract
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In this study, an organic solvent tolerant bacterial strain was isolated. This strain was identified as Pseudomonas sp. strain S5, and was shown to degrade BTEX (Benzene, Toluene, Ethyl-Benzene, and Xylene). Strain S5 generates an organic solvent-tolerant lipase in the late logarithmic phase of growth. Maximum lipase production was exhibited when peptone was utilized as the sole nitrogen source. Addition of any of the selected carbon sources to the medium resulted in a significant reduction of enzyme production. Lower lipase generation was noted when an inorganic nitrogen source was used as the sole nitrogen source. This bacterium hydrolyzed all tested triglycerides and the highest levels of production
<br><br>were observed when olive oil was used as a natural triglyceride. Basal medium
<br><br>containing Tween 60 enhanced lipase production to the most significant degree. The
<br><br>absence of magnesium ions (Mg2+) in the basal medium was also shown to stimulate lipase production. Meanwhile, an alkaline earth metal ion, Na+, was found to stimulate the production of S5 lipase.
- Biodegradation of Hydrocarbon Contamination by Immobilized Bacterial Cells
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Raja Noor Zaliha Abd. Rahman , Farinazleen Mohamad Ghazali , Abu Bakar Salleh , Mahiran Basri
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J. Microbiol. 2006;44(3):354-359.
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DOI: https://doi.org/2376 [pii]
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Abstract
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This study examined the capacity of immobilized bacteria to degrade petroleum hydrocarbons. A mixture of hydrocarbon-degrading bacterial strains was immobilized in alginate and incubated in crude oil-contaminated artificial seawater (ASW). Analysis of hydrocarbon esidues following a 30-day incubation period demonstrated that the biodegradation capacity of the microorganisms was not compromised by the immobilization. Removal of n-alkanes was similar in immobilized cells and control cells. To test reusability, the immobilized bacteria were incubated for sequential increments of 30 days. No decline in biodegradation capacity of the immobilized consortium of bacterial cells was noted over its repeated use. We conclude that immobilized hydrocarbon-degrading bacteria represent a promising application in the bioremediation of hydrocarbon-contaminated areas.
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